Detection of G-type density in promoter sequence of colon cancer oncogenes and tumor suppressor genes

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Aim of study: Leukemia has different subtypes, which present unique clinical and molecular characteristics. MLL (Mixed Lineage Leukemia) is one of the new different subtypes than AML and ALL. Materials and Methods:Genomic characterization is the main key understanding the differences of MLL by analysis of differential gene expression, methylation patterns and mutational spectra that were compared and analyzed between MLL and AML types (n=197).Results: According to the genomic characterization of MLL, differentially expressed 114 genes were selected and 37 of them targeted genes having more than 2 fold expression change, including HOXA9, CFH, DDX4, MSH4, MSMB, TWIST1, ZSWIM2, POU6F2. To measure the aberrant methylation is the second genomic characterization of this research because the rearrangements of MLL gene leading to aberrant methylation. The methylation data were compared between cancer and control, so high methylated genes have been detected between MLL and AML types. The methylation loci were categorized into two groups: ≥ 10 fold difference and ≥ 5 and ≤ 10 fold difference. Some of the genes high methylated more than one location such as; RAET1E, HSD17B2, RNASE11, DGK1, POU6F2, NAGS, PIK3C2G, GADL1, and KRT13. In addition to that, analysis of somatic mutation gives us that CFH has the highest point mutation 9,92%. Conclusion:Overall, the MLL genomic characterization shows that it is different than AML and exhibits a unique molecular and biological phenotype and point to new possible targetable genes for future treatment of MLL leukemia are two important values.

Section: Discussionmentioning

confidence: 86%

Section: Methylation and Gene Expressionmentioning

confidence: 99%

New Possible Targetable Genes for Future Treatment of Mixed Lineage Leukemia

Dogan¹

2017

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“…The main idea of the method is to compare the target genes between dead and alive patients. t-test has been applied to the gene expression values to find the statistically significant P-value between dead and alive dataset [14][15].…”

Section: Methodsmentioning

confidence: 99%

Analysis of Gaucher Disease Responsible Genes in Colorectal Adenocarcinoma

Karataş¹,

Turan²

2016

Gaucher disease is a hereditary genetic abnormality which defects the pathway of sphingolipid catabolism. The mutation of GBA gene which encodes lysosomal β-glucosidase enzyme is the main characteristics of the disease also is observed in different cancer types. To find the relation between the disease and colon adenocarcinoma, the responsible gene expression of Gaucher disease was analyzed. The gene expression of colon adenocarcinoma was compared between death and alive patients and analyzed statistically to profile the differences between Gaucher disease genes expression changes. GBA, GBA2, GBA3, SCARB2 and PSAP have the maximum genetic alteration which is observed in colon adenocarcinoma.

“…It demonstrates that the sex difference is constitutive and the women do not achieve male levels in health [5][6][7]. Biostatistical studies help us to understand many things from cancer studies to human physiology and life sciences [9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Comparison of the Hemoglobin Amount between Old and Young Persons in Bosnia and Herzegovina

Dogan¹,

Mermer²

2017

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Hemoglobin is a unique protein, which is responsible for oxygen and carbon dioxide transportation all the body. The protein location is inside the erythrocytes and the special oval shape makes it easily pass through blood walls to supply oxygen to the tissues and organs. It is supposed that the hemoglobin amount could change depending on the person`s age, gender or nationality. We designed a research to see the molecular differences among Bosnian and Turkish young person`s whose age interval is 18-23 and old person`s age interval is 43-65. Totally 300 person`s, 50 from each Bosnian/ Turkish Female/Male and 50 old male and 50 old female were selected for the research. The students` the hemoglobin amount has been recorded individually and presented in a table. As a result of the measurement, The Turkish females average has the lowest hemoglobin Turkish males average shows the maximum amount of hemoglobin, 12.01 g/dl and 14.65 g/dl respectively. When the female gets older their the hemoglobin amount increase in their blood, 7.3% in Bosnian and 12.2% in Turkish. On the other side, the male blood the hemoglobin amount is almost similar by aging, Bosnian male hemoglobin just increase 0.74%, but in Turkish male 1.3% decrease. The result shows that female the hemoglobin amount is affected by age more than male.