Detection of Fusarium head blight resistance QTL in a wheat population using bulked segregant analysis

Abstract

A population of 218 recombinant inbred lines (RILs) was developed from the cross of two wheat (Triticum aestivum L.) cultivars, 'Ning 894037' and 'Alondra'. Ning 894037 has resistance to Fusarium head blight (FHB) and Alondra is moderately susceptible. Response of the RILs and their parental lines to FHB infection was evaluated with point inoculation in four experiments both in greenhouse and in field conditions. Distribution of disease severity in the population is continuous, indicating quantitative inheritance of resistance to FHB. Bulked segregant analysis and QTL mapping based on simple sequence repeat (SSR) markers revealed three chromosome regions that are responsible for FHB resistance. A chromosome region on 3BS accounted for 42.5% of the phenotypic variation for FHB resistance. Additional QTLs were located on chromosomes 2D and 6B. These three QTLs jointly accounted for 51.6% of the phenotypic variation. SSR markers linked to the QTLs influencing resistance to FHB have potential for use in breeding programs.

Effect of cleistogamy on Fusarium head blight resistance in wheat

“…Second, the parental genotype of RILs in the QTLs on chromosomes 3BS and 5AS affected the FHB spread, percentage of FDKs, and concentration of DON. The positive effect of these QTLs on FHB resistance is consistent with the results of many studies conducted against various genetic backgrounds and environments (Anderson et al 2001, Buerstmayr et al 2003, McCartney et al 2007, Miedaner et al 2006, Nishio et al 2008, Shen et al 2003, Waldron et al 1999, Wilde et al 2007. Our findings confirmed that these QTLs are indispensable to increase FHB resistance in wheat.…”

Evaluation of Fusarium Head Blight Resistance in Wheat and the Development of a New Variety by Integrating Type I and II Resistance

“…6). The positive effect of these QTLs on FHB resistance is consistent with the results of many previous studies conducted under various genetic backgrounds and environments 1,8,27,31,35,42,50,52,62 . Our findings confirmed that the genotype of QTL, which is located on 3BS of 'Saikai 165' and 'Sumai 3,' is vital for increasing FHB resistance in wheat.…”

Chromosomal localization of selected SCARs converted from new RAPD, ISSR and R-ISSR markers linked to rye (Secale cereale L.) tolerance to nutrient deprivation stress identified using bulk segregant analysis

“…BSA has been successfully used in the mapping of single major genes (Barua et al 1993;Villar et al 1996;Quarrie et al 1999;Shen et al 2003), but a combination of BSA and the candidate genes approach could be very effective in discovering genes with a significant effect on QTLs (Angaji, 2009). …”