Detection of FUS-1 (OXA-85), a Class D Beta-lactamase from Fusobacterium nucleatum Subspecies Polymorphum in Nigeria

Nwaokorie, Francisca O.

doi:10.9734/bmrj/2013/3599

Cited by 2 publications

(2 citation statements)

References 15 publications

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“…In previous studies on patients with refractory periodontitis, very low proportions (<2%) were shown to harbor bacteria resistant to metronidazole, a common nitroimidazole antibiotic (Rams et al 2014). Another study showed that the nim gene was not detected in periodontitis samples, regardless of the source (tongue, subgingival sample, or deep pockets) (Nwaokorie et al 2013).…”

Section: Drug Resistance Mechanismsmentioning

confidence: 99%

Drug Resistance and Gene Transfer Mechanisms in Respiratory/Oral Bacteria

et al. 2018

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Growing evidence suggests the existence of new antibiotic resistance mechanisms. Recent studies have revealed that quorum-quenching enzymes, such as MacQ, are involved in both antibiotic resistance and cell-cell communication. Furthermore, some small bacterial regulatory RNAs, classified into RNA attenuators and small RNAs, modulate the expression of resistance genes. For example, small RNA sprX, can shape bacterial resistance to glycopeptide antibiotics via specific downregulation of protein SpoVG. Moreover, some bacterial lipocalins capture antibiotics in the extracellular space, contributing to severe multidrug resistance. But this defense mechanism may be influenced by Agr-regulated toxins and liposoluble vitamins. Outer membrane porin proteins and efflux pumps can influence intracellular concentrations of antibiotics. Alterations in target enzymes or antibiotics prevent binding to targets, which act to confer high levels of resistance in respiratory/oral bacteria. As described recently, horizontal gene transfer, including conjugation, transduction and transformation, is common in respiratory/oral microflora. Many conjugative transposons and plasmids discovered to date encode antibiotic resistance proteins and can be transferred from donor bacteria to transient recipient bacteria. New classes of mobile genetic elements are also being identified. For example, nucleic acids that circulate in the bloodstream (circulating nucleic acids) can integrate into the host cell genome by up-regulation of DNA damage and repair pathways. With multidrug resistant bacteria on the rise, new drugs have been developed to combate bacterial antibiotic resistance, such as innate defense regulators, reactive oxygen species and microbial volatile compounds. This review summaries various aspects and mechanisms of antibiotic resistance in the respiratory/oral microbiota. A better understanding of these mechanisms will facilitate minimization of the emergence of antibiotic resistance.

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Section: Drug Resistance Mechanismsmentioning

confidence: 99%

Drug Resistance and Gene Transfer Mechanisms in Respiratory/Oral Bacteria

et al. 2018

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“…The periodontal pathogens responsible for this undergo haematological translocation from the oral cavity to cause extra-oral infections (12). Fusobacterium nucleatum is an oral commensal associated with gingivitis, periodontitis, and oral cancer (13,14). It is implicated in metastatic infections involving the heart valves (15), liver (16), brain, joints (17), and septic thrombophlebitis in extra-pharyngeal locations (2), and the uterus (14), It is also isolated commonly in intrauterine infections (14).…”

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confidence: 99%

Evaluation of Fusobacterium nucleatum in Amniotic Fluid of Women with Preterm Labour

Nwaokorie,

Okunade,

Edet

et al. 2023

Babcock Univ. Med. J.

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Objective: The effect of Fusobacterium nucleatum, an oral commensal, on pregnancy outcomes is of global concern. This study screened for F. nucleatum and the fadA gene in amniotic fluid obtained from women presenting with preterm labour. Methods: This is a cross-sectional pilot study. Amniotic fluid was collected from women with preterm labour at Lagos University Teaching Hospital and analysed using conventional polymerase chain reaction (PCR). I6SrRNA species-specific primers were used to assess the presence of F. nucleatum species and fadA genes for virulence potentials. Questionnaire-based data obtained was analysed using SPSS version 20.0. and amplicon analysis was measured on base pairs. Results: Fourty participants had preterm deliveries between April and August 2018. Of these 34 (85%) were enrolled, out of which 25 amniotic fluid samples (AF) were collected. The mean age of the participants was 30.5 years. Among them, 10 (29.4%) were primigravida, and 24 (70.6%) were multigravida. Six (1.8%) of the women had a history of gestational diabetes, and four (1.2%) had hypertension. Only 4 (1.2%) were aware that bacteria could cause preterm labour. 11 (32%) visited the dentist during the period of their pregnancy. There was no amplification of 16SrRNA or the fadA genes in the tested samples. Conclusions: This study showed that preterm delivery was more common in multigravida pregnant women aged 31-35 years. Although some of the samples were cloudy and purulent, Fusobacterium nucleatum species-specific and virulent fadA genes were not detected in amniotic fluid obtained from the population of women with preterm labour studied.

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Detection of FUS-1 (OXA-85), a Class D Beta-lactamase from Fusobacterium nucleatum Subspecies Polymorphum in Nigeria

Cited by 2 publications

References 15 publications

Drug Resistance and Gene Transfer Mechanisms in Respiratory/Oral Bacteria

Drug Resistance and Gene Transfer Mechanisms in Respiratory/Oral Bacteria

Evaluation of Fusobacterium nucleatum in Amniotic Fluid of Women with Preterm Labour

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