Detection of fungicide resistance to fludioxonil and tebuconazole in <i>Fusarium pseudograminearum</i>, the causal agent of Fusarium crown rot in wheat

Zhang, Na; Xu, Yiying; Zhang, Qi; Zhao, Ling; Zhu, Yanan; Wu, Yanhui; Li, Zhen; Yang, Wenxiang

doi:10.7717/peerj.14705

Cited by 8 publications

(7 citation statements)

References 39 publications

(46 reference statements)

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“…The baseline sensitivity of F. pseudograminearum to tebuconazole (0.2328 ± 0.0840 μg/mL) was determined by in vitro testing of 83 isolates . Consequently, the findings of the current investigation may serve as a reference point for the surveillance of alterations in the susceptibility of F.…”

Section: Discussionmentioning

confidence: 95%

Mefentrifluconazole-Resistant Risk and Resistance-Related Point Mutation in FpCYP51B of Fusarium pseudograminearum

Li,

Ru,

Zhang

et al. 2024

J. Agric. Food Chem.

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Mefentrifluconazole, a triazole fungicide, exhibits remarkable efficacy in combating Fusarium spp. The mean EC50 value of mefentrifluconazole against 124 isolates of Fusarium pseudograminearum was determined to be 1.06 μg/mL in this study. Fungicide taming produced five mefentrifluconazole-resistant mutants with resistance factors ranging from 19.21 to 111.34. Compared to the original parental isolates, the fitness of three resistant mutants was much lower, while the remaining two mutants displayed enhanced survival fitness. There was evidence of positive cross-resistance between tebuconazole and mefentrifluconazole. Mefentrifluconazole resistance in F. pseudograminearum can be conferred by FpCYP51BL144F, which was identified in four mutants according to molecular docking and site-directed transformation experiments. Overexpression of FpCYP51s was also detected in the resistant mutants. In conclusion, mefentrifluconazole has a low-to-medium resistance risk in F. pseudograminearum, and the L144F mutation in FpCYP51B and the increased expression level of FpCYP51s may be responsible for mefentrifluconazole resistance in F. pseudograminearum.

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Section: Discussionmentioning

confidence: 95%

Mefentrifluconazole-Resistant Risk and Resistance-Related Point Mutation in FpCYP51B of Fusarium pseudograminearum

Li,

Ru,

Zhang

et al. 2024

J. Agric. Food Chem.

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“…7,8 Chemical fungicide application is the most effective measure for controlling FCR because of the limited resistance genes in wheat. Some existing fungicides have been screened for FCR control; for example, hexaconazole, 9 carbendazim, 10 kresoxim-methyl, 11 azoxystrobin, 12 phenamacril and difenoconazole, 13,14 all of which exhibit good control efficiency. Unfortunately, recent studies have shown that several fungicides at certain concentrations could enhance DON biosynthesis in Fusarium spp., such as carbendazim, 15 azoxystrobin 16 and difenoconazole.…”

Section: Introductionmentioning

confidence: 99%

Inhibitory activity to Fusarium spp. and control potential for wheat Fusarium crown rot of a novel succinate dehydrogenase inhibitor cyclobutrifluram

Li,

Dai,

Tang

et al. 2024

Pest Management Science

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BACKGROUNDWheat Fusarium crown rot (FCR) is a serious problem primarily caused by Fusarium pseudograminearum, a pathogenic agent known to produce mycotoxins, including deoxynivalenol (DON). Cyclobutrifluram, a novel succinate dehydrogenase inhibitor devised by Syngenta, has immense potential to control both nematodes and Fusarium diseases. However, its efficacy in combating Fusarium species, its ability to prevent and reverse the detrimental effects of FCR, and its impact on the production of DON by F. pseudograminearum are yet to be fully ascertained.RESULTSCyclobutrifluram exhibited substantial inhibitory activity against Fusarium species, with half‐maximal effective concentration values ranging from 0.0021–0.0647 μg mL−1. It demonstrated significant inhibitory activity toward three developmental stages of F. pseudograminearum, F. graminearum and F. asiaticum. Furthermore, cyclobutrifluram showed both protective and curative activities against FCR and was rapidly absorbed by roots and transported to wheat stems and leaves. Cyclobutrifluram could also decrease DON production by F. pseudograminearum.CONCLUSIONThis investigation has revealed the potential of cyclobutrifluram as a formidable candidate fungicide, particularly in its ability to effectively combat FCR and other Fusarium‐related ailments. This novel compound has exceptional pathogen‐fighting capabilities, coupled with remarkable systemic translocation properties and a notable ability to reduce the production of DON. © 2023 Society of Chemical Industry.

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“…Wheat is one of the most important cereal crops that sustains more than 1/3 of the world's population, and maintaining consistent production is therefore critical to global food security. It has been estimated that wheat cultivation in China covers a total area of 2.34 million hectares with an annual production of 13.43 million tons per year [ 1 , 2 ]. However, recent research has found that infection with Fusarium crown rot (FCR) resulted in an average yield loss of 36,000 tons per year in Henan Province between 2017 and 2021 alone, with the highest annual loss being 44,000 tons [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Survey of prothioconazole sensitivity in Fusarium pseudograminearum isolates from Henan Province, China, and characterization of resistant laboratory mutants

Zhou,

Jiao,

Han

et al. 2024

BMC Plant Biol

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Background Fusarium crown rot (FCR) is one of the most significant diseases limiting crop production in the Huanghuai wheat-growing region of China. Prothioconazole, a triazole sterol 14α-demethylation inhibitor (DMI) fungicide developed by the Bayer Crop Protection Company, is mainly registered for the prevention and control of wheat powdery mildew and stripe rust (China Pesticide Information Network). It is known to exhibit high activity against F. pseudograminearum, but further research, particularly regarding the potential for fungicide resistance, is required before it can be registered for the control of FCR in China. Results The current study found that the baseline sensitivity of 67 field isolates of F. pseudograminearum collected between 2019 and 2021 ranged between 0.016–2.974 μg/mL, with an average EC50 value of 1.191 ± 0.720 μg/mL (mean ± SD). Although none of the field isolates exhibited signs of resistance, three highly resistant mutants were produced by repeated exposure to prothioconazole under laboratory conditions. All of the mutants were found to exhibit significantly reduced growth rates on potato dextrose agar (PDA), as well as reduced levels of sporulation, which indicated that there was a fitness cost associated with the resistance. However, inoculation of wounded wheat coleoptiles revealed that the pathogenicity of the resistant mutants was little affected or actually increased. Molecular analysis of the genes corresponding to the prothioconazole target protein, FpCYP51 (FpCYP51A, FpCYP51B, and FpCYP51C), indicated that the resistant mutants contained three conserved substitutions (M63I, A205S, and I246V) that were present in the FpCYP51C sequence of all three mutants, as well as several non-conserved substations in their FpCYP51A and FpCYP51B sequences. Expression analysis revealed that the presence of prothioconazole (0.1 μg/mL) generally resulted in reduced expression of the three FpCYP51 genes, but that the three mutants exhibited more complex patterns of expression that differed in comparison to their parental isolates. The study found no evidence of cross-resistance between prothioconazole and any of the fungicides tested including three DMI fungicides tebuconazole, prochloraz, and flutriafol. Conclusions Taken together these results not only provide new insight into the resistant mechanism and biological characteristics associated with prothioconazole resistance in F. pseudograminearum, but also strong evidence that prothioconazole could provide effective and sustained control of FCR, especially when applied in combination with other fungicides.

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Detection of fungicide resistance to fludioxonil and tebuconazole in Fusarium pseudograminearum, the causal agent of Fusarium crown rot in wheat

Cited by 8 publications

References 39 publications

Mefentrifluconazole-Resistant Risk and Resistance-Related Point Mutation in FpCYP51B of Fusarium pseudograminearum

Mefentrifluconazole-Resistant Risk and Resistance-Related Point Mutation in FpCYP51B of Fusarium pseudograminearum

Inhibitory activity to Fusarium spp. and control potential for wheat Fusarium crown rot of a novel succinate dehydrogenase inhibitor cyclobutrifluram

Survey of prothioconazole sensitivity in Fusarium pseudograminearum isolates from Henan Province, China, and characterization of resistant laboratory mutants

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