Detection of fructophilic lactic acid bacteria (FLAB) in bee bread and bee pollen samples and determination of their functional roles

Abstract

In this study, bee bread and bee pollen samples were characterized in terms of presence of fructophilic lactic acid bacteria (FLAB). Following discrimination and identification of FLAB strains, their functional roles were determined. Genotypic characterization of FLAB strains revealed the presence of 27 distinct strains belonging to Lactobacillus kunkeei (21), Fructobacillus fructosus (3), Lactobacillus plantarum (2), and Leuconostoc mesenteroides (1) strains. In terms of functional roles, all FLAB strains demonstrated high levels of autoaggregation and hydrophobicity properties whereas L. kunkeei and F. fructosus strains did not tolerate bile salts and low pH conditions.A low level of antibiotic resistance was observed among FLAB strains and importantly FLAB strains demonstrated high levels of antibacterial and antifungal activities where strain specific properties played roles. The highest exopolysaccharide (EPS) production yields were obtained for L. kunkeei AP15, AP37 and L. mesenteroides AP-40 which produced 2.79, 2.01, and 5.37 g/L EPS, respectively. Practical applicationsBee bread and bee pollen are among beneficial bee products produced and consumed in all over the world. Production of bee bread relies on the fermentation process of bee pollen where a special LAB group FLAB plays roles. Despite the increment of the importance of these products for human consumption, there is a lack of knowledge for the microbial composition of these products. In this study, FLAB strains were isolated and identified from bee bread and bee pollen samples, and their technofunctional role was determined. The FLAB strains would be evaluated as starter cultures for production of bee products under industrial conditions.How to cite this article: Ispirli H, Dertli E. Detection of fructophilic lactic acid bacteria (FLAB) in bee bread and bee pollen samples and determination of their functional roles. J

Bee Bread as a Promising Source of Bioactive Molecules and Functional Properties: An Up-To-Date Review

“… [ 21 ] Fructose, Glucose, Turanose, and Maltose HPLC-DAD Romania and India Five samples (Brassicaceae, Poaceae, Myrtaceae, Rutaceae, Asteraceae, Fabaceae, Tiliaceae, Fabaceae, Rosaceae, Plantaginaceae, Fabaceae, Asteraceae, Lamiaceae, Salicaceae, Rosaceae, and Fagaceae) [ 22 ] Fructose, Glucose, Sucrose, and Maltose HPLC coupled with an ELSD Malaysia Four multifloral samples ( Mimosa pudica, Sphagneticolatrilobata, Bidens ilosa, Cassia sp, Areca catechu, Peltophorumpterocarpum, Phaleria capitata, Cassia siamea, Citrus aurantifolia, and Ageratum conyzoides ) [ 11 ] Microorganisms Lactobacillus kunkeei RAPD-PCR Turkey Four samples (not identified). [ 60 ] Cladosporium, Penicillium, Alternaria, Monilinia, Sclerotina, Ascosphaera, Toxicocladosporium, Botrytis, Pseudopithomyces, Camerosporium, Paraconiothyrium, Podosphaera, Golovinomyces, Paraphaeosphaeria, Periconia, and Septoriella NGS-sequencing Bulgaria Four samples (not identified). [ 54 ] Apilactobacilluskunkeei, Lactiplantibacillus plantarum, Fructobacillusfructosus, Levilactobacillus brevis, and Lactobacillus delbrueckii PCR-DGGE analysis RAPD-PCR analysis Italy Twelve samples (not identified) [ 55 ] Organic acids Oxalic acid …”

A Snapshot Picture of the Fungal Composition of Bee Bread in Four Locations in Bulgaria, Differing in Anthropogenic Influence

“…It provides easily digestible carbohydrates, proteins, fats, minerals, and vitamins for the bee larvae and the adult bees, but also acts as a functional food (feed), possessing antioxidant and anti-inflammatory properties [1][2][3]. Even though many studies in the last years have been focused on the bacterial composition of bee bread [4][5][6], those on fungal composition are rather scarce despite the publication of some of them dating from the '80s [7]. An exception is the study of Tauber et al [8].…”

Biotechnological Processes Simulating the Natural Fermentation Process of Bee Bread and Therapeutic Properties—An Overview

“…It was shown that mice treated with LGG had lower symptoms frequency and higher survival rates than control. Also, increased mRNA expression of IL-8, TNF, and MCP-1 (monocyte chemotactic protein) were noticed in Ispirli and Dertli ( 183 ).…”