Detection of free‐living and plankton‐bound vibrios in coastal waters of the Adriatic Sea (Italy) and study of their pathogenicity‐associated properties

Baffone, Wally; Tarsi, R.; Pane, Luigi; Campana, Raffaella; Repetto, Barbara; Mariottini, Gian Luigi; Pruzzo, Carla

doi:10.1111/j.1462-2920.2006.01011.x

Cited by 85 publications

(79 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Higher V. parahaemolyticus densities may be the result of a rise in temperature, because warmer conditions are expected to promote the proliferation of V. parahaemolyticus cells on the zooplankton exoskeleton (Kaneko and Colwell, 1973, 1975, 1978Baffone et al, 2006). However, no tendency of seawater temperature to produce higher observed loads of V. parahaemolyticus and zooplankton was observed in this study.…”

Section: Discussioncontrasting

confidence: 49%

“…Among these species, Vibrio parahaemolyticus inhabits natural environments with moderate salinity and warm temperature conditions, and its life cycle has consequently been associated with estuarine systems (Kaneko and Colwell, 1973, 1975, 1978Joseph et al, 1982). Moreover, V. parahaemolyticus has been regularly isolated from the microbiome of different marine organisms, such as corals (Chimetto et al, 2008), fish (Cabrera-Garcia et al, 2004;Herrera et al, 2006;Terzi et al, 2009), molluscs (Blackstone et al, 2003;Martinez-Urtaza et al, 2008b), sponges (Hoffmann et al, 2010), shrimp (Cabanillas-Beltrán et al, 2006) and zooplankton (Kaneko and Colwell, 1973;Baffone et al, 2006). The interaction with planktonic organisms has a central role in the pelagic ecology of Vibrio populations.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Ecological determinants of the occurrence and dynamics of Vibrio parahaemolyticus in offshore areas

et al. 2011

View full text Add to dashboard Cite

The life cycle of Vibrio parahaemolyticus has been conventionally associated with estuarine areas characterized by moderate salinity and warm seawater temperatures. Recent evidence suggests that the distribution and population dynamics of V. parahaemolyticus may be shaped by the existence of an oceanic transport of communities of this organism mediated by zooplankton. To evaluate this possibility, the presence of V. parahaemolyticus in the water column of offshore areas of Galicia was investigated by PCR monthly over an 18-month period. Analysis of zooplankton and seawater showed that the occurrence of V. parahaemolyticus in offshore areas was almost exclusively associated with zooplankton and was present in 80% of the samples. The influence of environmental factors assessed by generalized additive models revealed that the abundance and seasonality of V. parahaemolyticus in zooplankton was favoured by the concurrence of downwelling periods that promoted the zooplankton patchiness. These results confirm that offshore waters may be common habitats for V. parahaemolyticus, including strains with virulent traits. Additionally, genetically related populations were found in offshore zooplankton and in estuaries dispersed along 1500 km. This finding suggests that zooplankton may operate as a vehicle for oceanic dispersal of V. parahaemolyticus populations, connecting distant regions and habitats, and thereby producing impacts on the local community demography and the spread of Vibrio-related diseases.

show abstract

Section: Discussioncontrasting

confidence: 49%

Section: Introductionmentioning

confidence: 99%

Ecological determinants of the occurrence and dynamics of Vibrio parahaemolyticus in offshore areas

et al. 2011

View full text Add to dashboard Cite

show abstract

“…Vibrios have been detected on a large variety of biological surfaces, especially animals (Thompson et al 2004, Baffone et al 2006, Main et al 2015, and are also associated with various types of organic particles of non-animal origin (Lyons et al 2007, Froelich et al 2013. Recently, there has been evidence that vibrios can remain free-living (Mourino-Perez et al 2003, Worden et al 2006, Eiler et al 2006, although little is known on the factors determining whether they remain free-living versus particle-attached (Takemura et al 2014).…”

Section: Are Vibrios Found Preferentially Attached To Particles?mentioning

confidence: 99%

“…In the NW Mediterranean, Vibrio infection together with temperature might have contributed to mass mortality events of benthic invertebrates (Vezzulli et al 2010). On the Adriatic Sea coast, Vibrio expressing pathogenicity-associated properties were found mainly in the warmer months (Baffone et al 2006).…”

Section: Relationships Between Vibrio and Environmental Factorsmentioning

confidence: 99%

Variability of planktonic and epiphytic vibrios in a coastal environment affected by <em>Ostreopsis</em> blooms

Bellés-Garulera¹,

Vila²,

Borrull³

et al. 2016

Sci. Mar.

View full text Add to dashboard Cite

Summary:Vibrios include several pathogenic bacteria that occur in aquatic environments. The presence of Vibrio has been assessed in many ecosystems by culture-based techniques. However, little is known on the contribution of vibrios in the sea, especially in areas subject to harmful algal blooms. A preliminary study in Sant Andreu de Llavaneres beach (NW Mediterranean) showed the presence of some Vibrio species during a recurrent bloom of the harmful benthic dinoflagellate Ostreopsis cf. ovata. In order to establish the importance of vibrios in a coastal area of the NW Mediterranean and to study the association with the dinoflagellate, we conducted a sampling monitoring for one year to quantify the concentration of vibrios both in the water (free-living and attached to particles) and in the epiphytic community of macroalgae. The aims were 1) to evaluate the relative abundance of Vibrio in the epiphytic and in the planktonic bacterial community, 2) to assess the percentage of free-living and attached vibrios in the planktonic community, and 3) to determine whether the presence of vibrios is associated with the blooms of the toxic dinoflagellate Ostreopsis or with other environmental parameters. For this purpose, a CARD-FISH molecular probe was applied for the specific detection of bacteria belonging to the genus Vibrio. Cells were quantified and the abundance of both particles and bacteria attached to particles were assessed. The maximum Vibrio concentration (1.3x10 4 cells ml -1 and 1.4x10 6 cells g -1 FW, for planktonic and epiphytic samples, respectively) was detected in September. Free-living vibrios contributed 0.38±0.24% to the total free-living planktonic community and 1.12±0.28% to the epiphytic bacterial community. However, their contribution was particularly high in the planktonic community attached to particles (17.37±20.49%). Although in the planktonic community Vibrio was found preferentially free-living (82.63±20.01%), particles are a niche for vibrios, since in particles vibrios may represent up to 72% of the total attached bacterial community. Abundance of planktonic Vibrio was correlated with Ostreopsis concentration and it is likely that they play a role in the wound infections suffered by beach users during the bloom.Keywords: Vibrio; bacteria; particles; Mediterranean; HAB; dinoflagellates. Variabilidad de vibrios planctónicos y epifíticos en un ambiente costero afectado por proliferaciones de OstreopsisResumen: El género Vibrio incluye a varias bacterias patogénicas que se encuentran en ecosistemas acuáticos. La presencia de Vibrio se ha estimado en muchos ecosistemas mediante técnicas basadas en cultivos. Sin embargo, se conoce poco sobre la contribución de vibrios en el mar, especialmente en áreas afectadas por proliferaciones algales nocivas. Un estudio preliminar en la playa de Sant Andreu de Llavaneres (Mediterráneo NO) mostró la presencia de algunas especies de Vibrio durante una proliferación recurrente del dinoflagelado béntico nocivo Ostreopsis cf. ovata. Para poder establecer la rel...

show abstract

“…infections are still complex and related to several factors including cytotoxins, enterotoxins, and lytic enzymes [276,277]. Adhesion ability to human epithelial cell lines (Hep-2 and Caco-2) and fish mucus [278] seem to be diffused among Vibrio alginolyticus strains and may represent a potential infection risk for aquatic stressed animals [279,280]. Snoussi et al [265] confirmed that V. alginolyticus strains isolated from a bathing and fishing area (Khenis, Centre of Tunisia) show a specific binding capability to gilthead sea bass and gilthead sea bream mucus.…”

Section: Fish Skin Mucosal Immunologymentioning

confidence: 99%

An Overview of the Immunological Defenses in Fish Skin

2012

View full text Add to dashboard Cite

The vertebrate immune system is comprised of numerous distinct and interdependent components. Every component has its own inherent protective value, and the final combination of them is likely to be related to an animal's immunological history and evolutionary development. Vertebrate immune system consists of both systemic and mucosal immune compartments, but it is the mucosal immune system which protects the body from the first encounter of pathogens. According to anatomical location, the mucosa-associated lymphoid tissue, in teleost fish is subdivided into gut-, skin-, and gill-associated lymphoid tissue and most available studies focus on gut. The purpose of this paper is to summarise the current knowledge of the immunological defences present in skin mucosa as a very important part of the fish immune system, serving as an anatomical and physiological barrier against external hazards. Interest in defence mechanism of fish arises from a need to develop health management tools to support a growing finfish aquaculture industry, while at the same time addressing questions concerning origins and evolution of immunity in vertebrates. Increased knowledge of fish mucosal immune system will facilitate the development of novel vaccination strategies in fish.

show abstract

Detection of free‐living and plankton‐bound vibrios in coastal waters of the Adriatic Sea (Italy) and study of their pathogenicity‐associated properties

Cited by 85 publications

References 25 publications

Ecological determinants of the occurrence and dynamics of Vibrio parahaemolyticus in offshore areas

Ecological determinants of the occurrence and dynamics of Vibrio parahaemolyticus in offshore areas

Variability of planktonic and epiphytic vibrios in a coastal environment affected by <em>Ostreopsis</em> blooms

An Overview of the Immunological Defenses in Fish Skin

Contact Info

Product

Resources

About