Detection of foot-and-mouth disease virus infection-associated antigen antibodies: comparison of the enzyme-linked immunosorbent assay and agar gel immunodiffusion tests

Abstract

Alonso, A., Gomes, M.P.D., Martins, M.A. and Sondahl, M.S., 1990. Detection of foot-and-mouth disease virus infection-associated antigen antibodies: comparison of the enzyme-linked immunosorbent assay and agar gel immunodiffusion tests. Prev. Vet. Med.,.A liquid-phase enzyme-linked immunosorbent assay (ELISA) was compared with the standard agar gel immunodiffusion test (AGID) to identify and quantify antibodies against foot-and-mouth disease (FMD) virus infection-associated (VIA) antigen. A total of 3181 cattle sera were tested. Of these sera, 1885 were from cattle which had not been exposed to FMD. A total of 1296 sera were either from cattle which were experimentally exposed to FMD virus or from cattle involved in field outbreaks. The results indicate that the ELISA has the same specificity as the AGID test, but is more efficient in detecting cattle exposed to FMD virus. The ELISA technique will probably prove to be a more satisfactory test in support of the prevention, control and eradication programs for the disease.

Expression of Foot-and-Mouth Disease Virus Non-Structural Protein, 3D in Insect Cells and its Application in Detection of Anti-FMDV Antibodies

“…NSPs viz. 2C [13], 3A, 3B, 3AB, 3ABC and 3D [2] have been used by different investigators using either an enzyme-linked immunotransfer blot (EITB) [5,4] or ELISA formats [5,16,23,24]. However, there is still no international reference test (gold standard) for NSP based detection assays.…”

Development and Evaluation of an Enzyme-Linked Immunosorbent Assay for Detection, Typing, and Subtyping of Vesicular Stomatitis Virus

“…The detector produced in this way does not require blocking with NBS. 2 The proposed ELISA increases by 10% the positive results obtained by the CF 50 test in the examination of OS samples. Previously, virus could be isolated by cell culture inoculation only in approximately 5% of the negative CF 50 OS samples from VS endemic areas tested by direct examination.…”

“…The plates were subsequently blocked for 1 hr at 24-25 C with 1% ovoalbumin b in Dulbecco's phosphate-buffered saline free of Ca and Mg (PBS) as described previously. 2 The antigens were tested in 50-µ1 aliquots of the original suspension and left to react for 1 hr at 37 C on a plate shaker. 3) Guinea pig sera and conjugate (goat IgG anti-guinea pig IgG/ peroxidase 7 ) were added successively in 50.-µ1 aliquots of the optimal dilution and incubated at 37 C for 30 min on a plate shaker.…”

“…2 1) ELISA plates a were coated overnight at 4 C with 100 µ1 of rabbit antisera and normal rabbit serum optimally diluted in carbonate/bicarbonate buffer, pH 9.6. The plates were subsequently blocked for 1 hr at 24-25 C with 1% ovoalbumin b in Dulbecco's phosphate-buffered saline free of Ca and Mg (PBS) as described previously.…”

“…Sera were inactivated at 56 C for 30 min, aliquoted, and stored at -20 C. Guinea pig antisera (for detection in the ELISA and CF,, test) were prepared by infecting and hyperimmunizing the animals with infectious virus previously adapted to this species according to the method described previously. 2,4 The rabbit and guinea pig VIND polyvalent antiserum (VIND-P) was obtained by mixing proportional aliquots of monovalent VIND-1, VIND-2, and VIND-3 according to their CF,, test titers.…”