Detection of foodborne Salmonella Typhimurium outbreaks

Arnedo-Pena, Alberto; Bellido-Blasco, Juan B.; Romeu-Garcia, Ma Angeles; Meseguer-Ferrer, Noemí

doi:10.1016/j.eimc.2017.01.001

Cited by 6 publications

(3 citation statements)

References 9 publications

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“…While identifying “hot-spots” of Salmonella specific serovar distribution would be desirable, the substantial differences in disease dynamics among Salmonella serotypes must be taken into account for a correct epidemiological interpretation of results. Arnedo-Pena et al [4] highlight some differential characteristics regarding the incubation period, outbreak duration, attack rate, hospitalization rate, probability of reaching a microbiological diagnosis, and human behavior that influence the variation in disease dynamics of foodborne outbreaks of S. Typhimurium and S. Enteritidis in Spain. It would be desirable to characterize also other serovars identified in animal health surveillance results (i.e., in pigs: S. Rissen and S. Derby) to target interventions.…”

Section: Discussionmentioning

confidence: 99%

“…Consequently, there was a significant reduction of Salmonella in humans and poultry during the period 2008–2016 [3], particularly due to S. Enteritidis. In contrast, there was an apparent increase of notifications of S. Typhimurium cases, which are less likely associated with the consumption of eggs and egg products and more predominantly found in pork and pork products [4].…”

Section: Introductionmentioning

confidence: 99%

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Salmonella Surveillance Systems in Swine and Humans in Spain: A Review

Martínez‐Avilés¹,

Garrido‐Estepa²,

Álvarez

et al. 2019

Veterinary Sciences

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Non-typhoid salmonellosis is a common and problematic foodborne zoonotic disease in which pork and pork products can be an important potential source of infection. To prevent this disease, important efforts to monitor the situation in the main source, livestock, are conducted in most developed countries. In the European Union, European Food Safety Agency (EFSA) and European Center for Disease Control (ECDC) compile information at the member-state level, even though important differences in production systems and surveillance systems exist. Here, Salmonella surveillance systems in one of the main sources of foodborne salmonellosis, swine, and humans in Spain were reviewed to identify potential gaps and discuss potential ways of integration under a “One-Health” approach. Despite the extensive information generated through the surveillance activities, source attribution can be only routinely performed through ad-hoc outbreak investigations, and national reports on human outbreaks do not provide sufficiently detailed information to gain a better understanding of the epidemiology of the pathogen. Human and animal monitoring of Salmonella would benefit from a better exchange of information and collaboration. Analysis of spatio-temporal trends in livestock and humans could help to identify likely sources of infection and to target surveillance efforts in areas with higher prevalence or where specific strains are found.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Salmonella Surveillance Systems in Swine and Humans in Spain: A Review

Martínez‐Avilés¹,

Garrido‐Estepa²,

Álvarez

et al. 2019

Veterinary Sciences

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“…FCM has been used for detection of pathogens and for bacteriological quality control of milk [71,72,73,74] and the physiological functions of the bacterial cells in pineapple and mango juice [70]. A Gram-staining technique combining staining with two fluorescent stains, Oregon Green-conjugated wheat germ agglutinin (WGA) and hexidium iodide (HI) followed by flowcytometric detection were described.…”

Section: Backerymentioning

confidence: 99%

Updates on the applications of flow cytometry in microbiology

2019

Lett Appl NanoBioSci

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Flow cytometry (FCM) was first developed for medical and clinical applications such as hematology and oncology. Although these areas still account for the vast majority of publications on this technique, during the past few years it has been also introduced in other areas, such as optimization and monitoring of biotechnological and environmental processes, pharmacology, toxicology, bacteriology and virology. In the food and drinks industries, the time required for conventional microbiology tests can lead to substantial delays in product release to the market. FCM has been used in conjunction with viability markers for rapid counting of yeast, molds and bacterial cells, including foodborne pathogens and microbial contaminants, in food products as well as for monitoring and improving the final products quality. FCM is an excellent tool, still unexplored in clinical microbiology, allowing for detection of cellular and non-cellular components in different clinical specimens, the study of antimicrobial activity, allowing for rapid and direct antimicrobial susceptibility testing and for the investigation of resistance mechanisms. Recent FCM developments important for addressing questions in environmental microbiology include the study of microbial physiology under environmentally relevant conditions, the development of new methods to identify active microbial populations and to isolate previously uncultured microorganisms and of high-throughput autofluorescence bioreporter assays. Moreover, the technological advancements will make possible the miniaturisation and automation of FCM devices, allowing to revolutionize their applications in the near future. The purpose of this minireview is to update the current applications of FCM in different fields of applied microbiology, and to highlight the main advantages and pitfalls for each of them.

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