Detection of five potentially periodontal pathogenic bacteria in peri-implant disease: A comparison of PCR and real-time PCR

Schmalz, Gerhard; Tsigaras, Sandra; Rinke, Sven; Kottmann, Tanja; Haak, Rainer; Ziebolz, Dirk

doi:10.1016/j.diagmicrobio.2016.04.003

Cited by 13 publications

(10 citation statements)

References 33 publications

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“… 9 Various anaerobic bacteria, including Porphyromonas gingivalis ( P. gingivalis ), Fusobacterium nucleatum ( F. nucleatum ), and Aggregatibacter actinomycetemcomitans ( A. actinomycetemcomitans ), have been shown to be implicated in peri-implantitis. 10 , 11 Because of the growing development of bacterial resistance to antibiotics, there is a considerable interest in the preparation of antimicrobial materials. 12 Therefore, there is great interest in developing an antimicrobial biomimetic implant surface that could prevent bacterial colonization from the outset.…”

Section: Introductionmentioning

confidence: 99%

Physicochemical and Antibacterial Characterization of a Novel Fluorapatite Coating

Alhilou¹,

Do²,

Mizban³

et al. 2016

ACS Omega

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Peri-implantitis remains the major impediment to the long-term use of dental implants. With increasing concern over the growth in antibiotic resistance, there is considerable interest in the preparation of antimicrobial dental implant coatings that also induce osseointegration. One such potential coating material is fluorapatite (FA). The aim of this study was to relate the antibacterial effectiveness of FA coatings against pathogens implicated in peri-implantitis to the physicochemical properties of the coating. Ordered and disordered FA coatings were produced on the under and upper surfaces of stainless steel (SS) discs, respectively, using a hydrothermal method. Surface charge, surface roughness, wettability, and fluoride release were measured for each coating. Surface chemistry was assessed using X-ray photoelectron spectroscopy and FA crystallinity using X-ray diffraction. Antibacterial activity against periodontopathogens was assessed in vitro using viable counts, confocal microscopy, and scanning electron microscopy (SEM). SEM showed that the hydrothermal method produced FA coatings that were predominately aligned perpendicular to the SS substrate or disordered FA coatings consisting of randomly aligned rodlike crystals. Both FA coatings significantly reduced the growth of all examined bacterial strains in comparison to the control. The FA coatings, especially the disordered ones, presented significantly lower charge, greater roughness, and higher area when compared to the control, enhancing bacteria–material interactions and therefore bacterial deactivation by fluoride ions. The ordered FA layer reduced not only bacterial viability but adhesion too. The ordered FA crystals produced as a potential novel implant coating showed significant antibacterial activity against bacteria implicated in peri-implantitis, which could be explained by a detailed understanding of their physicochemical properties.

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Section: Introductionmentioning

confidence: 99%

Physicochemical and Antibacterial Characterization of a Novel Fluorapatite Coating

Alhilou¹,

Do²,

Mizban³

et al. 2016

ACS Omega

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“…A difference at species level has been identified between human and dogs in periodontitis, however, so far as we know, the comparison of species associated with dental implants in dogs and humans remains to be investigated. The common periodontal pathogens, P. gingivalis , T. denticola , T. forsythia , F. nucleatum , P. intermedia , and A. actinomycetemcomitans , were screened as potential peri‐implant pathogen candidates by PCR or RT‐PCR in humans . Therefore, they were selected as target bacteria for evaluation instead in this study.…”

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confidence: 99%

“…The common periodontal pathogens, P. gingivalis, T. denticola, T. forsythia, F. nucleatum, P. intermedia, and A. actinomycetemcomitans, were screened as potential peri-implant pathogen candidates by PCR or RT-PCR in humans. 10,11 Therefore, they were selected as target bacteria for evaluation instead in this study.…”

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confidence: 99%

Detection of T. forsythia and other important bacteria in crestal and subcrestal implants with ligature‐induced peri‐implant infection in dogs

Zhu

Meng

Huang

et al. 2018

Journal of Periodontology

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“…This shortcoming renders sophisticated statistical analysis like post-hoc power calculation or a regression analysisin order to test for confounders pointless. Anyhow, since the semi-quantitative DNA-strip test has been used in several previous studies it was shown to be a reliable test for bacterial analysis [ 36 , 45 ]. Therefore, it constitutes a viable fundament for a basic analysis of how the oral flora might change after tonsillectomy as determined in this study.…”

Section: Discussionmentioning

confidence: 99%

What is the influence of tonsillectomy on the level of periodontal pathogens on the tongue dorsum and in periodontal pockets

et al. 2018

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BackgroundFor periodontal treatment, the full mouth disinfection approach suggests disinfection of oral soft tissues, such as tongue and tonsils concomitant to scaling and root planning since patients might benefit from treatment of these oral niches either. Periodontopathogenes in tonsillar tissue support this hypothesis. This prospective controlled clinical study investigated the change in the oral flora of patients who underwent tonsillectomy. Pockets were tested for eleven bacterial species before and six weeks after the surgical intervention.MethodsFifty generally healthy adults were included in this study. The test group consisted of 25 patients with tonsillectomy. The control group included 25 patients with otorhinolarynologic surgery without involvement of the oral cavity. Clinical parameters such as probing pocket depth, bleeding-on-probing index and plaque index were registered the evening before surgery. Also bacterial samples from the gingival sulcus and dorsum linguae were taken, and an additional sample from the removed tonsils in the test group. Six weeks after the intervention microbial samples of pockets and tongue were taken again. Data were tested for significant differences using Wilcoxon rank and Whitney-u-test.ResultsNo relevant intra- or intergroup differences were found for the change of the eleven investigated species.ConclusionBased on the results of the present study, tonsillectomy does not seem to have an immediate relevant effect on the bacterial flora of tongue or periodontium. This study design was approved by the ethical committee of Zurich (KEK-ZH-Nr.2013–0419).Trial registrationThe trial was retrospectively registered in the German Clinical Trials Register (DRK00014077) on February 20, 2018.

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Detection of five potentially periodontal pathogenic bacteria in peri-implant disease: A comparison of PCR and real-time PCR

Cited by 13 publications

References 33 publications

Physicochemical and Antibacterial Characterization of a Novel Fluorapatite Coating

Physicochemical and Antibacterial Characterization of a Novel Fluorapatite Coating

Detection of T. forsythia and other important bacteria in crestal and subcrestal implants with ligature‐induced peri‐implant infection in dogs

What is the influence of tonsillectomy on the level of periodontal pathogens on the tongue dorsum and in periodontal pockets

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