Detection of feline kobuviruses in diarrhoeic cats, Italy

Martino, Barbara Di; Profio, Federica Di; Melegari, Irene; Marsilio, Fulvio; Martella, Vito

doi:10.1016/j.vetmic.2015.01.012

Cited by 15 publications

(36 citation statements)

References 24 publications

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“…We investigated 197 fecal samples of which 28 (14.2%) were positive for kobuvirus. The prevalence rate is similar to previous reports in South Korea (15.4%, 6/39), Italy (13.5%, 5/37) and southern China (9.9%, 8/81) (Chung et al 2013;Di Martino et al 2015b;Lu et al 2018), suggesting that kobuvirus widely circulates in domestic cats in northeast China. The prevalence of kobuvirus in diarrhoeic cats (19.1%, 20/105) is significantly higher than that in healthy cats (8.7%, 8/92), similar to a previous study by Cho, et al (Cho et al 2014).…”

Section: Discussionsupporting

confidence: 89%

“…In the past few years, the circulations of kobuvirus in cats had been reported in South Korea, Italy and southern China (Chung et al 2013;Di Martino et al 2015b;Lu et al 2018). However, the related data of FeKoV in other countries and regions is lacking.…”

Section: Discussionmentioning

confidence: 99%

“…In a study by Cho, et al, it was demonstrated that kobuvirus widely circulated in domestic cats and was associated with viral diarrhea (Cho et al 2014). Recently, FeKoV infection in cats was reported in Italy (Di Martino et al 2015b). In 2018, Lu et al first reported the circulation of FeKoV in diarrhoeic cats in southern China.…”

Section: Introductionmentioning

confidence: 99%

“…FeKoV RNA was found in 8 domestic cats with diarrhea, but was undetected in healthy cats (Lu et al 2018). However, only four complete genomes of FeKoV strains, including FK-13 (Cho et al 2014), 12D240 (Choi et al 2015), TE/52/IT/2013 (Di Martino et al 2015b) and WHJ-1 (Lu et al 2018), have been sequenced until now. Furthermore, there is no data of kobuvirus infections in cats in northeast China.…”

Section: Introductionmentioning

confidence: 99%

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Detection and genetic characterization of kobuvirus in cats: The first molecular evidence from Northeast China

Niu

Wang

et al. 2019

Infection, Genetics and Evolution

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A B S T R A C TFeline kobuvirus (FeKoV), a novel picornavirus of the genus kobuvirus, was initially identified in the feces of cats with diarrhea in South Korea in 2013. To date, there is only one report of the circulation of kobuvirus in cats in southern China. To investigate the presence and genetic variability of FeKoV in northeast China, 197 fecal samples were collected from 105 cats with obvious diarrhea and 92 asymptomatic cats in Shenyang, Jinzhou, Changchun, Jilin and Harbin regions, Northeast China, and viruses were detected by RT-PCR with universal primers targeting all kobuviruses. Kobuvirus was identified in 28 fecal samples with an overall prevalence of 14.2% (28/197) of which 20 samples were co-infected with feline parvovirus (FPV) and/or feline bocavirus (FBoV). Diarrhoeic cats had a higher kobuvirus prevalence (19.1%, 20/105) than asymptomatic cats (8.7%, 8/ 92). By genetic analysis based on partial 3D gene, all kobuvirus-positive samples were more closely related to previous FeKoV strains with high identities of 90.5%-97.8% and 96.6%-100% at the nucleotide and amino acid levels. Additionally, phylogenetic analysis based on the complete VP1 gene indicated that all FeKoV strains identified in this study were placed into a cluster, which separated from other reference strains previously reported, and three identical amino acid substitutions were present at the C-terminal of the VP1 protein for these FeKoV strains. Furthermore, two complete FeKoV polyprotein genomes were successfully obtained from two positive samples and designated 16JZ0605 and 17CC0811, respectively. The two strains shared 92.9%-94.9% nucleotide identities and 96.8%-98.4% amino acid identities to FeKoV prototype strains. Phylogenetic analysis indicated that FeKoVs were clustered according to their geographical regions, albeit with limited sequences support. This study provides the first molecular evidence that FeKoV circulates in cats in northeast China, and these FeKoVs exhibit genetic diversity and unique evolutionary trend.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Detection and genetic characterization of kobuvirus in cats: The first molecular evidence from Northeast China

Niu

Wang

et al. 2019

Infection, Genetics and Evolution

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“…In the samples yielding amplicons of the expected sizes, the presence of NoV was confirmed using norovirus-specific primer pair JV12Y-JV13I (Vennema et al, 2002). All the fecal samples were also tested by PCR or RT-PCR for feline parvovirus (FPV) (Buonavoglia et al, 2001), feline enteric coronavirus (FECV) (Gunn-Moore et al, 1998) and feline kobuviruses (FeKoV) (Di Martino et al, 2015).…”

Section: Screening By Rt-pcr and Pcrmentioning

confidence: 99%

A novel feline norovirus in diarrheic cats

Martino

Profio

Melegari

et al. 2016

Infection, Genetics and Evolution

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By screening a collection of fecal samples from young cats housed in three different shelters in South Italy, noroviruses (NoVs) were found in 3/48 (6.2%) specimens of animals with enteritis signs while they were not detected in samples collected from healthy cats (0/57). Upon sequence analysis of the short RNA-dependent RNA polymerase (RdRp) region, the three strains displayed the highest nucleotide (nt) and amino acid (aa) identities to the prototype GIV.2 strain lion/Pistoia/387/06/ITA (91.0-93.0% nt and 97.0-98.0% aa). The sequence of ~3.4-kb portion at the 3' end of the genome of a NoV strain, TE/77-13/ITA, was determined. In the full-length ORF2, encoding the VP1 capsid protein, the virus was genetically closest to the canine GVI.2 NoV strains C33/Viseu/2007/PRT and FD53/2007/ITA (81.0-84.0% nt and 93.0-94.0% aa identities), suggesting a recombination nature, with the cross-over site being mapped to the ORF1-ORF2 junction. Based on the full-length VP1 amino acid sequence, we classified the novel feline NoV, together with the canine strains Viseu and FD53, as a genotype 2, within the genogroup GVI. These findings indicate that, as observed for GIV NoV, GVI strains may infect both the canine and feline host. Unrestricted circulation of NoV strains in small carnivores may provide the basis for quick genetic diversification of these viruses by recombination. Interspecies circulation of NoVs in pets must also be considered when facing outbreaks of enteric diseases in these animals.

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Identification and genome characterization of a novel feline picornavirus proposed in the Hunnivirus genus

Huang

Chen

et al. 2019

Infection, Genetics and Evolution

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Detection of feline kobuviruses in diarrhoeic cats, Italy

Cited by 15 publications

References 24 publications

Detection and genetic characterization of kobuvirus in cats: The first molecular evidence from Northeast China

Detection and genetic characterization of kobuvirus in cats: The first molecular evidence from Northeast China

A novel feline norovirus in diarrheic cats

Identification and genome characterization of a novel feline picornavirus proposed in the Hunnivirus genus

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