Detection of feline calicivirus, feline herpesvirus 1 and Chlamydia psittaci mucosal swabs by multiplex RT-PCR/PCR

Sykes, Jane E.; Allen, Joanne L.; Studdert, Virginia P.; Browning, Glenn F.

doi:10.1016/s0378-1135(01)00340-6

Cited by 70 publications

(91 citation statements)

References 32 publications

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“…It is also possible that there may have been sensitivity issues with the PCRs used, particularly for FCV. Indeed the FCV RT-PCR performed with the conserved polymerase p30 gene 11 was more sensitive (116 positives detected) than that performed on the hypervariable region of the capsid protein gene 12 (79 positives detected) ( Table 1). Thus using both primer pairs for FCV clearly helped maximise the overall sensitivity of detection, but because of the high sequence variability seen in FCV there may still have been other strains which were not detectable with the primer pairs used.…”

mentioning

confidence: 99%

Feline herpesvirus 1 and feline calicivirus infections in a heterogeneous cat population of a rescue shelter

Zicola

Saegerman

Quatpers

et al. 2009

Journal of Feline Medicine and Surgery

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Feline herpesvirus 1 (FeHV-1) and feline calicivirus (FCV), associated with upper respiratory tract disease, are highly prevalent in cats worldwide. With the aim to investigate the importance of feline respiratory viruses in a heterogeneous population of cats, samples were taken in a rescue shelter in Liège, Belgium, between March 2005 and August 2006. Reverse transcription polymerase chain reaction (RT-PCR) and polymerase chain reaction (PCR) were performed to diagnose FCV and FeHV-1 infection in the sampled cats. The prevalence rate (33.1%) was higher for FCV than for FeHV-1 (20.1%) whereas prevalence rate of co-infection with both viruses was 10%. Gingivitis was more common in FCV infections (odds ratio (OR) ¼ 2.83) whereas respiratory signs were more often observed with FeHV-1 infections. The average age was significantly higher in FCV positive cats (38 months) than in FeHV-1 positive cats (29.9 months). The second and the fourth quarters of the year and the two first quarters were significantly more at risk than the others in the case of FeHV-1 and FCV infection, respectively. Age was found to be a confounding factor. High prevalence of both infections strengthens the importance of applying hygienic and preventive measures in rescue shelters where cats with an unknown status of vaccination are introduced.

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mentioning

confidence: 99%

Feline herpesvirus 1 and feline calicivirus infections in a heterogeneous cat population of a rescue shelter

Zicola

Saegerman

Quatpers

et al. 2009

Journal of Feline Medicine and Surgery

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“…Isolation has traditionally been used to identify these pathogens. Recently, PCR tests which are more sensitive than isolation have been developed for the detection of FHV and C. felis (1,(6)(7)(8)10).…”

mentioning

confidence: 99%

Detection of Chlamydophila felis and Feline Herpesvirus by Multiplex Real-Time PCR Analysis

2003

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Chlamydophila felis and feline herpesvirus (FHV) are pathogens commonly associated with feline respiratory and ocular disease. A real-time multiplex PCR assay was developed to allow detection of these organisms, together with feline 28S ribosomal DNA, in a single tube. Of 538 ocular swab samples tested, 123 were positive for FHV, 97 were positive for C. felis, and 16 were positive for both pathogens.

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“…RT-PCR의 최 종산물은 0.5 µg/mL(w/v) ethidium bromide (Bioneer)가 포 함된 2% agarose (SeaKem LE, Rockland, ME, USA)로 전 기영동하여 확인하였다 9,30,31) . .…”

Section: Rt-pcrunclassified

Quantitative Analysis of Feline Calicivirus Inactivation using Real-time RT-PCR

Jeong¹,

Kim²

2014

Journal of Food Hygiene and Safety

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-Norovirus causes acute gastroenteritis in all age groups and its food poisoning outbreaks are rapidly increasing in Korea. Reverse transcription-polymerase chain reaction (RT-PCR) is most widely used for the rapid detection of foodborne viruses due to high sensitivity. However, the false positive results of RT-PCR obtained against already inactivated viruses could be a serious drawbacks in food safety area. In this study, we investigated a method to yield true positive RT-PCR results only with alive viruses. To decompose the RNA genes from dead viruses, the enzymatic treatments composed of proteinse K and Ribonuclease A were applied to the sanitized and inactivated virus particles. Another aim of this study was to quantify the efficiencies of several major sanitizing treatments using realtime RT-PCR. Feline calicivirus (FCV) that belongs to the same Caliciviridae family with norovirus was used as a surrogate model for norovirus. The initial level of virus in control suspension was approximately 10 4 PFU/mL. Most of inactivated viruses treated with the enzymatic treatment for 30 min at 37 o C were not detected in RT-PCR, Quantification results to verify the inactivation efficiencies of sanitizing treatments using real-time RT-PCR showed no false positive in most cases. We could successfully develope a numerical quantification process for the inactivated viruses after major sanitizing treatments using real-time RT-PCR. The results obtained in this study could provide a novel basis of rapid virus quantification in food safety area.

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Detection of feline calicivirus, feline herpesvirus 1 and Chlamydia psittaci mucosal swabs by multiplex RT-PCR/PCR

Cited by 70 publications

References 32 publications

Feline herpesvirus 1 and feline calicivirus infections in a heterogeneous cat population of a rescue shelter

Feline herpesvirus 1 and feline calicivirus infections in a heterogeneous cat population of a rescue shelter

Detection of Chlamydophila felis and Feline Herpesvirus by Multiplex Real-Time PCR Analysis

Quantitative Analysis of Feline Calicivirus Inactivation using Real-time RT-PCR

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