Detection of fatty acids, fatty aldehydes, phospholipids, glycolipids and cholesterol on thin-layer chromatograms stained with malachite green

Teichman, R.; Takei, Gerald H.; Cummins, James M.

doi:10.1016/s0021-9673(00)83178-7

Cited by 31 publications

(8 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the content of the inclusions could be preserved by adding lipid‐retaining malachite green during the fixation process, especially when the overall fixation times were extended (Fig. 1D and E) (Teichman et al ., 1974). Regardless of the preservation of their content, all lipid bodies were surrounded in thin sections by an electron‐dense layer of about 4–5 nm thickness (Figs 1D and 5D and E).…”

Section: Resultsmentioning

confidence: 98%

Lipid body formation plays a central role in cell fate determination during developmental differentiation of Myxococcus xanthus

Hoiczyk¹,

Ring²,

McHugh³

et al. 2009

Molecular Microbiology

View full text Add to dashboard Cite

SummaryCell differentiation is widespread during the development of multicellular organisms, but rarely observed in prokaryotes. One example of prokaryotic differentiation is the Gram-negative bacterium Myxococcus xanthus. In response to starvation, this gliding bacterium initiates a complex developmental programme that results in the formation of spore-filled fruiting bodies. How the cells metabolically support the necessary complex cellular differentiation from rodshaped vegetative cells into spherical spores is unknown. Here, we present evidence that intracellular lipid bodies provide the necessary metabolic fuel for the development of spores. Formed at the onset of starvation, these lipid bodies gradually disappear until they are completely used up by the time the cells have become mature spores. Moreover, it appears that lipid body formation in M. xanthus is an important initial step indicating cell fate during differentiation. Upon starvation, two subpopulations of cells occur: cells that form lipid bodies invariably develop into spores, while cells that do not form lipid bodies end up becoming peripheral rods, which are cells that lack signs of morphological differentiation and stay in a vegetative-like state. These data indicate that lipid bodies not only fuel cellular differentiation but that their formation represents the first known morphological sign indicating cell fate during differentiation.

show abstract

Section: Resultsmentioning

confidence: 98%

Lipid body formation plays a central role in cell fate determination during developmental differentiation of Myxococcus xanthus

Hoiczyk¹,

Ring²,

McHugh³

et al. 2009

Molecular Microbiology

View full text Add to dashboard Cite

show abstract

“…The multidevelopment procedure for separation of lipids (64,71) and polyunsaturated acids (11) is a significant improvement over previous methods. Visualization of chromatograms has been improved by the use of a malachite green spray reagent for lipids (149) and a reagent containing molybdate for phospholipids (157). Fluorometric analysis of lipids after spraying chromatograms with rhodamine 6G is useful in quantitative estimation (122).…”

Section: Thin-layer Chromatography and Liquidmentioning

confidence: 99%

Fatty acids of the genus Bacillus: an example of branched-chain preference.

Kaneda¹

1977

Bacteriological Reviews

269

147

View full text Add to dashboard Cite

nally methyl-branched fatty acids, 13-methyltetradecanoic (iso-C15) and 15-methylhexadecanoic (iso-C,7), in Bacillus subtilis (natto) (138), and 12-methyltetradecanoic (anteiso-C,5) acid in a species of Sarcina (4), was first reported. After these reports, extensive studies conducted in my laboratory led to the discovery of three additional branched-chain fatty acids; 12methyltridecanoic (iso-C14), 14-methylpentadecanoic (iso-C16), and 14-methylhexadecanoic (anteiso-C,7) acids in the lipids of B. subtilis (76). These branched-chain acids are grouped in three series, based on their biosynthetic relationships (28, 77, 79, 80, 92, 102, 119) (Fig. 1). The definitions of iso and anteiso permit only iso methyl-substituted fatty acids (an iso ethyl would be an anteiso methyl of one-higher chain number), but would allow both methyl and ethyl as anteiso substitutions. Only the methyl anteiso substituent has so far been found in 391

show abstract

“…The problem of the loss of cellular materials during fixation for ultrastructural studies is especially acute in the case of lipids: up to 40% of the total choline of rabbit spermatozoa is extracted during glutaraldehyde fixation (Teichman, Cummins & Takei, 1974b) and phospholipid losses from other tissues are well documented (Morgan & Huber, 1967;McGee-Russell & De Bruijn, 1968;Cope & Williams, 1968, 1969. We have already demonstrated (Teichman et al, 1972(Teichman et al, , 1974) that the fixation of rabbit spermatozoa in glutaraldehydecontaining malachite green results in the stabilization and staining of at least one glutaraldehyde-soluble phospholipid, choline plasmalogen. In the present work, the varied morphology of MGA material suggests that malachite green is staining a number of different classes of lipid.…”

Section: Discussionmentioning

confidence: 85%

“…found that mammalian sperma¬ tozoa fixed in glutaraldehyde containing malachite green or pyronine retained highly osmiophilic material which was lost during conventional fixation. Spot tests and thin-layer chromatography aimed at determining the staining affinity of malachite green showed that it binds strongly with fatty acids, glycolipids, phospholipids and cholesterol but has no affinity for glycerylphosphorylcholine (GPC) (Teichman, Takei & Cummins, 1974). The 'mala¬ chite-green-affinity' (MGA)-material in rabbit spermatozoa has been shown to consist largely of choline plasmalogen (Teichman, Cummins & Takei, 1974a).…”

Section: Introductionmentioning

confidence: 99%

Light and Electron Microscope Studies on Lipid Stained by Malachite Green in the Male Reproductive Tract of the Rabbit, Hamster and Mongoose

Cummins¹,

Bernstein²,

Teichman³

1974

Reproduction

View full text Add to dashboard Cite

Reproductive tracts from male rabbits, hamsters and mongooses were fixed in glutaraldehyde containing malachite green. The technique stains phospholipids, glycolipids, fatty acids and cholesterol, and is known to retain a lipid within spermatozoa which is lost during conventional fixation. In rabbit spermatozoa, the lipid has been identified as choline plasmalogen, which accumulates in the post \ x=r eq-\ acrosomal region of the head during epididymal transit. In testes, malachite green stained Leydig cell granules and cytoplasmic inclusions in maturing spermatids. Large quantities of stained material were evident in the epididymides and accessory glands, including basal cell lysosomes which increased in size and frequency after castration, supranuclear cytosome-like bodies, material associated with smooth endoplasmic reticulum, vesicles distributed throughout the principal cells of the epithelium and material around the stereocilia of the epididymis. Amounts of material in the epididymal lumen increased markedly from caput to cauda, indicating active secretion.

show abstract

Detection of fatty acids, fatty aldehydes, phospholipids, glycolipids and cholesterol on thin-layer chromatograms stained with malachite green

Cited by 31 publications

References 2 publications

Lipid body formation plays a central role in cell fate determination during developmental differentiation of Myxococcus xanthus

Lipid body formation plays a central role in cell fate determination during developmental differentiation of Myxococcus xanthus

Fatty acids of the genus Bacillus: an example of branched-chain preference.

Light and Electron Microscope Studies on Lipid Stained by Malachite Green in the Male Reproductive Tract of the Rabbit, Hamster and Mongoose

Contact Info

Product

Resources

About