Detection of extended spectrum beta-lactamases and resistance in members of the Enterobacteriaceae family isolated from healthy sheep and dogs in Umuarama, Paraná, Brazil

Oliveira, Patrícia Alves de; Moura, Rodrigo Assunção; Rodrigues, Graziela Vendrame; Lopes, Karoline Franciani Cardoso; Zaniolo, Melissa Marchi; Rubio, Kariny Aparecida Jardim; Dias, Eduardo Herrera; Oliveira, Luiz Antonio de; Chideroli, Roberta Torres; Gonçalves, Daniela Dib

doi:10.5433/1679-0359.2016v37n2p829

Cited by 4 publications

(6 citation statements)

References 32 publications

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“… Virulence genes Ref. Africa CTX-M-1, CTX-M-15, SHV-12, TEM-135 [ 20 , [111] , [112] , [113] , [114] , [116] , [117] , [118] ] No data America CTX-M-1, CTX-M-2, CTX-M-3, CTX-M-8, CTX-M-9, CTX-M-14, CTX-M-15, CTX-M-24, CTX-M-27, CTX-M-55, CTX-M-65, CTX-M-106, CTX-M-115, CTX-M-123, CTX-M-169, CTX-M-202, SHV-2, SHV-3, SHV-12, TEM-5, TEM-30, TEM-33, TEM-181 [ 10 , 35 , 37 , [46] , [47] , [48] , [49] , [50] , [51] , [52] , [53] , [54] , [55] , [56] , [57] , [58] ] afa/draBC, air, cba, cma, cnf1, cvaC, eilA, fimH, focA, fyuA, gad, hlyA, hlyD, ibeA, ipfA, ireA. iroN, iutA, iss, kpsMTII, kpsMTK5, malX, mchF, PAI, papA, papC, papE, papGIII, rfc, sfa/focDE, tsh, traT [ 10 , 21 , 51 , 55 , 56 ] Asia CTX-M-1, CTX-M-2, CTX-M-3, CTX-M-8, CTX-M-9, CTX-M-13, CTX-M-14, CTX-M-15, CTX-M-24, CTX-M-27, CTX-M-28, CTX-M-55, CTX-M-57, CTX-M-64, CTX-M-65, CTX-M-90, CTX-M-104, CTX-M-116, CTX-M-123, CTX-M-127, CTX-M-174, SHV-12, SHV-190, TEM-30 [ 8 , 11 , 13 , 15 , 31 , 36 , [119] , [120] , [121] , ...…”

Section: Resultsmentioning

confidence: 99%

Global prevalence and molecular characterization of extended-spectrum β-lactamase producing-Escherichia coli in dogs and cats – A scoping review and meta-analysis

et al. 2021

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Antimicrobial resistance (AMR) represents a major threat to human and animal health. Part of the AMR dimension is the circulation of extended-spectrum β-lactamases producing- Escherichia coli (ESBL- E. coli ), which is now commonly reported among companion animals. However, the global perspective of the prevalence and population structure of ESBL- E. coli circulating in dogs and cats has not been estimated limiting our understanding of their role in the dissemination of ESBL- E. coli . The aim of this study was to compare the prevalence of ESBL- E. coli between dogs and cats and across countries through meta-analysis. We also performed a scoping review to summarize the current knowledge on ESBL genes and E. coli clones circulating among companion animals. A total of 128 studies published in PubMed, Web of Science, and Scopus up to April 2020 were selected and contained information on prevalence and/or molecular characterization of ESBL genes and ESBL- E. coli clones. Our review shows an increase in the number of publications between 2000 and 2019, concentrated mainly in Europe. Prevalence varied across continents, ranging from 0.63% (Oceania) to 16.56% (Africa) in dogs and from 0% (Oceania) to 16.82% (Asia) in cats. Although there were twice as many studies reporting prevalence on dogs ( n = 61) than on cats ( n = 32), and only 9 studies focused exclusively on cats, our meta-analysis showed no difference in the global prevalence of ESBL- E. coli between dogs (6.87% [95% CI: 4.46–10.45%]) and cats (5.04% [95% CI: 2.42–10.22%]). A considerable diversity of ESBL genes ( n = 60) and sequence types (ST) ( n = 171) were recovered from companion animals. ESBL- E. coli encoded by CTX-M-15 (67.5%, 77/114) and SHV-12 (21.9%, 25/114), along with resistant strains of ST38 (22.7%, 15/66) and ST131 (50%, 33/66) were widespread and detected in all continents. While presence of ESBL- E. coli is widespread, the drivers influencing the observed ESBL- E. coli prevalence and the clinical relevance in veterinary medicine and public health along with economic impact of ESBL- E. coli infections among companion animals need to be further investigated.

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Section: Resultsmentioning

confidence: 99%

Global prevalence and molecular characterization of extended-spectrum β-lactamase producing-Escherichia coli in dogs and cats – A scoping review and meta-analysis

et al. 2021

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“…This is considered one of the biggest public health problems because infections are common. In some cases, the microorganisms are resistant to the available antimicrobial drugs [10,16,25,26,28].…”

Section: Introductionmentioning

confidence: 99%

“…The emergence of the selection of multidrugresistant strains is related to the inappropriate use of antimicrobials, besides their use as growth promotion and prophylaxis, in the case of veterinary medicine. In these cases, the doses of antibiotics used are lower than the therapeutic ones, which facilitate mutations and/or acquisition of resistance genes [4,11,16,25].…”

Section: Introductionmentioning

confidence: 99%

“…These enzymes hydrolyze the beta-lactam ring of the antibiotic structure, inactivating them. Currently, more than 370 ESBL variant are known [13,15,16,19]. Therefore, the aim of this study was to identify Enterobacteriaceae isolated from household cats (Felis silvestris catus), to evaluate their antibiotic resistance profiles and to detect extended-spectrum beta-lactamases (ESBL) in a city in the western region of the State of Parana, Brazil.…”

Section: Introductionmentioning

confidence: 99%

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Antimicrobial Resistance and Extended-Spectrum Beta-Lactamase Production in Enterobacteriaceae Isolates from Household Cats (Felis silvestris catus)

Andrade

Santos

Barbosa

et al. 2019

Acta Scientiae. Vet.

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Background:In Brazil, cats in households has recently increased dramatically, likely due to their lower space and care requirements. We need to know the health of these companion animal species, since they have behavioral patterns that make them an important link in the epidemiological chain. Extended spectrum beta-lactamase producer strains (ESBL) are resistant to penicillin, cephalosporin and monobactam, but they are susceptible to clavulanate. The goal of this study is to detect Enterobacteriaceae that produce extended spectrum beta-lactamase (ESBL) and evaluate the bacterial resistance profile in isolated cats (Felis silvestris catus) that live in a city located at west of Parana state, Brazil. Materials, Methods & Results:Swabs were aseptically collected from the anal orifice and oral cavity of 49 female domestic cats that were healthy upon clinical and physical examination, a minimum age of one year, weighing up to 3 kg, and had attended a veterinary clinic specializing in cats, in order to, later, perform the isolation and bacterial identification, antimicrobial sensibility phenotypic test and the phenotypic test to detect ESBL producer strains. From the 98 swabs collected it was possible to perform the bacterial isolation in 68 samples; 40.81% isolated from anal orifice and 28.57% isolated from oral cavity. From rectal and oral cavities 77.50% and 71.42% of the isolated were identified as Escherichia coli respectively, being 2.94% considered ESBL producer strains. In relation to bacterial resistance the antibiotics that shown more resistance in anal orifice were ampicillin, amoxicillin, nalidixic acid, sulfazotrim, tetracycline and aztreonam. In oral cavity they were ampicillin, amoxicillin, cefoxitin, amoxicillin + clavulanate, aztreonam, ceftriaxone and nalidixic acid; and the bacterial resistance index shown that 39.70% were considered high level risk. Discussion: Household cats have a very important role in society, since the benefits they provide to their owners are clear, however, it is worth pointing out that these animals also pose risks to human health, caused by the transmission of zoonoses and also the possibility of transfer of antimicrobial resistance genes between bacteria of animal and human origin, as well as between bacteria of the normal microbiota and pathogenic microorganisms of diferents origins. Therefore, it is important to understand the health of these companion animal species, because they exhibit behavioral patterns that make them an important link in the epidemiological chain of potentially infectious microorganisms, which may show antimicrobial resistance. Extended spectrum beta-lactamase producer strains (ESBL) are resistant to penicillin, cephalosporin and monobactam, but they are susceptible to clavulanate. These enzymes hydrolyze the beta-lactam ring of the antibiotic structure, inactivating them. Nowadays bacterial resistance is considered to be one of the greatest problems in public health worldwide, as infections and diseases outbraks are caused by multiresistant bacter...

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“…The 40.7% E. coli isolation prevalence in this study is higher than 17, 3.2, 24.5, 20, 1.9 and 3% ESBL-resistant/producing E. coli isolation prevalence reported in faecal samples of 53 healthy dogs in Mexico (Rocha-Gracia et al, 2015), oral swabs of 31 healthy dogs in Brazil (Oliveira et al, 2016), faecal samples of 109 healthy dogs in China (Sun et al, 2010), faecal/urine samples of companion animals in Chile (Moreno et al, 2008), 209 dog faecal deposits in Denmark (Damborg et al, 2015) and among 944 clinical E. coli isolates from companion animals in America (Shaheen et al, 2011), respectively. It is also higher than 16, 7, 6.6 and 2.6% ESBL-resistant/producing E. coli isolation prevalence in 100 extra-clinical faecal samples of dogs in Germany (Schaufler et al, 2015), among 445 clinical E. coli isolates from companion animals in United Kingdom (UK) (Timofte et al, 2016), faecal samples of 75 healthy dogs/cats (Costa et al, 2004) and 39 healthy dogs (Costa et al, 2008) reported in Portugal, respectively.…”

Section: Discussionmentioning

confidence: 45%

Prevalence and Antibiogram of Generic Extended-Spectrum β-Lactam-Resistant Enterobacteria in Healthy Dogs

Anyanwu¹,

Ugwu²,

EZEKWELU³

et al. 2017

Not Sci Biol

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This study was conducted to isolate generic extended-spectrum β-lactam-resistant enterobacteria from household dogs in Nigeria, and to determine the antibacterial resistance profile of the isolates. Rectal swabs were collected from 100, randomly selected, apparently healthy household dogs. Isolation of ESBL-resistant enterobacteria was done using Mac Conkey agar supplemented with 4 µg/ml of ceftazidime. Phenotypic characterization of the isolates to generic level was done following standard biochemical methods. Phenotypic resistance of the isolates to antibacterial agents was determined using the disc diffusion method. Out of 27 ESBL-resistant enterobacterial isolates, 40.7% were Escherichia coli, 37% were Klebsiella species, 18.5% were Salmonella species, while 3.7% was Proteus species. Resistance of the E. coli isolates was 81.8% to ampicillin, 27.2% to streptomycin, 54.5% to ciprofloxacin and tetracycline, 45.4% to enrofloxacin, 90.9% to sulphamethoxazole/trimethoprim, 9.1% to amoxicillin/clavulanic acid and 0% resistant to gentamicin. Resistance of the Klebsiella isolates was 80% to ampicillin, 20% to streptomycin and amoxicillin/clavulanic acid, 30% to ciprofloxacin and enrofloxacin, 60% to tetracycline, 90% to sulphamethoxazole/trimethoprim and 10% to gentamicin. Resistance of the Salmonella isolates was 100% to sulphamethoxazole/trimethoprim, 80% to gentamicin and ampicillin, 60% to streptomycin and tetracycline, 20% to amoxicillin/clavulanic acid, and 0% to ciprofloxacin and enrofloxacin. The Proteus isolate was resistant to streptomycin and gentamycin. All the isolates were resistant to ceftazidime and cefotaxime. Resistance of the isolates to more than 3 classes of antibacterial agents tested was 81.8% for E. coli, 70% for Klebsiella and 100% for Salmonella, respectively. This study has shown that household dogs in Nigeria, are colonized by ESBL-resistant Enterobacteriaceae and are potential reservoirs and disseminators of these organisms.

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Detection of extended spectrum beta-lactamases and resistance in members of the Enterobacteriaceae family isolated from healthy sheep and dogs in Umuarama, Paraná, Brazil

Cited by 4 publications

References 32 publications

Global prevalence and molecular characterization of extended-spectrum β-lactamase producing-Escherichia coli in dogs and cats – A scoping review and meta-analysis

Global prevalence and molecular characterization of extended-spectrum β-lactamase producing-Escherichia coli in dogs and cats – A scoping review and meta-analysis

Antimicrobial Resistance and Extended-Spectrum Beta-Lactamase Production in Enterobacteriaceae Isolates from Household Cats (Felis silvestris catus)

Prevalence and Antibiogram of Generic Extended-Spectrum β-Lactam-Resistant Enterobacteria in Healthy Dogs

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