Detection of Experimental Autoimmune Myocarditis in Rats by ¹¹¹ In Monoclonal Antibody Specific for Tenascin-C

Abstract

Radiolabeled anti-TNC Fab' may be useful for the noninvasive diagnosis of myocarditis.

Higher Serum Tenascin-C Levels Reflect the Severity of Heart Failure, Left Ventricular Dysfunction and Remodeling in Patients With Dilated Cardiomyopathy

“…As previously reported, TN-C is specifically expressed in the myocardial inflammatory lesions of myocarditis [16][17][18] and AMI. 12,15 Furthermore, serum TN-C levels are elevated in AMI patients, reflecting local expression of TN-C in the infarcted myocardium.…”

Noninvasive Assessment of Myocardial Inflammation by Cardiovascular Magnetic Resonance in a Rat Model of Experimental Autoimmune Myocarditis

“…16,17 Induction of the EAM rat model, fluorescence-activated cell-sorting analysis of inflammatory cellular infiltrates within EAM hearts, in vivo CMR protocol, histopathological examination, image analysis, MR image tracking of MNP-labeled macrophages in vivo, and summary of experiments are presented in detail in the online-only Data Supplement. The protocol was approved by the local institutional review committee on animal care (KBSI-AEC1009).…”

Dynamic Expression of Tenascin-C After Myocardial Ischemia and Reperfusion: Assessment by 125I-Anti–Tenascin-C Antibody Imaging

“…Labeling with 123 I can easily be performed in the same way as for 125 I, and 111 In labeling has already been achieved (9,17). Rapid blood clearance and retention of 125 I-TNC-Ab in the infarcted area, resulting in a decent target-to-blood ratio and good target-to-lung ratio, indicate the feasibility of in vivo imaging at 5 h or later after tracer injection.…”

“…Tenascin-C appears in the heart only in the early stages of embryonic development and is not normally expressed in adults. However, it reappears transiently in association with myocardial injury such as myocardial infarction (4)(5)(6), hibernating myocardium (7), myocarditis (6,8,9), and dilated cardiomyopathy (10,11). Its site-specific expression suggests that tenascin-C plays important roles during tissue remodeling but also that it can serve as an indicator of myocardial disease activity.…”

“…In previous animal studies on acute myocardial infarction induced by permanent ligation of a coronary artery, it was reported that tenascin-C appears transiently during the acute stage, plays several significant roles in myocardial tissue remodeling, and may be a potential candidate for molecular imaging (9,17). For clinical application of the imaging, it is essential to analyze the molecular dynamics in the model of myocardial ischemia and reperfusion that is the general scenario in the era of percutaneous coronary intervention in acute coronary syndrome.…”