Detection of Eubacterial 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductases from Natural Populations of Actinomycetes

Sigmund, J. M.; Clark, Desmond; Rainey, Frederick A.; Anderson, Annaliesa S.

doi:10.1007/s00248-002-2029-5

Cited by 19 publications

(13 citation statements)

References 24 publications

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“…Actinomycetes isolated from marine invertebrates have also been shown to produce previously reported HIs [45 ,46,47], however the phylogenetic relationships of these strains are unknown because of limited 16S sequence availability. A 2003 study by Sigmund et al [48] provided the first evidence that marine strains may be enriched in HI secondary metabolite production with the observation that all four hmgr positive strains, out of 385 screened, were from marine sources. Similarly, a 2010 study by Khan et al [35 ] identified six actinomycete strains containing the hmgr gene out of 523 marine-derived strains screened.…”

Section: Hi Producing Strains From the Marine Environmentmentioning

confidence: 97%

Hybrid isoprenoid secondary metabolite production in terrestrial and marine actinomycetes

Gallagher

Fenical

Jensen

2010

Current Opinion in Biotechnology

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Section: Hi Producing Strains From the Marine Environmentmentioning

confidence: 97%

Hybrid isoprenoid secondary metabolite production in terrestrial and marine actinomycetes

Gallagher

Fenical

Jensen

2010

Current Opinion in Biotechnology

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“…Orthologs of these six genes, arranged in the exact same order, are found in the biosynthetic gene cluster of furaquinocin (25), naphterpin (26), terepenticin (27), and BE 40644 (28). S. anulatus, there- fore, is one of the rare Streptomyces species that possesses the genes of the mevalonate pathway, a feature limited to ϳ1% of the strains of this genus (29). Directly adjacent to the genes of the mevalonate pathway, a gene designated ppzP was identified (Fig.…”

Section: Cloning Of a Biosynthetic Gene Cluster For Prenylatedmentioning

confidence: 99%

Aromatic Prenylation in Phenazine Biosynthesis

Saleh

Gust

Boll

et al. 2009

Journal of Biological Chemistry

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The bacterium Streptomyces anulatus 9663, isolated from the intestine of different arthropods, produces prenylated derivatives of phenazine 1-carboxylic acid. From this organism, we have identified the prenyltransferase gene ppzP. ppzP resides in a gene cluster containing orthologs of all genes known to be involved in phenazine 1-carboxylic acid biosynthesis in Pseudomonas strains as well as genes for the six enzymes required to generate dimethylallyl diphosphate via the mevalonate pathway. This is the first complete gene cluster of a phenazine natural compound from streptomycetes. Heterologous expression of this cluster in Streptomyces coelicolor M512 resulted in the formation of prenylated derivatives of phenazine 1-carboxylic acid. After inactivation of ppzP, only nonprenylated phenazine 1-carboxylic acid was formed. Cloning, overexpression, and purification of PpzP resulted in a 37-kDa soluble protein, which was identified as a 5,10-dihydrophenazine 1-carboxylate dimethylallyltransferase, forming a C-C bond between C-1 of the isoprenoid substrate and C-9 of the aromatic substrate. In contrast to many other prenyltransferases, the reaction of PpzP is independent of the presence of magnesium or other divalent cations. The K m value for dimethylallyl diphosphate was determined as 116 M. For dihydro-PCA, half-maximal velocity was observed at 35 M. K cat was calculated as 0.435 s ؊1 . PpzP shows obvious sequence similarity to a recently discovered family of prenyltransferases with aromatic substrates, the ABBA prenyltransferases. The present finding extends the substrate range of this family, previously limited to phenolic compounds, to include also phenazine derivatives.The transfer of isoprenyl moieties to aromatic acceptor molecules gives rise to an astounding diversity of secondary metabolites in bacteria, fungi, and plants, including many compounds that are important in pharmacotherapy. However, surprisingly little biochemical and genetic data are available on the enzymes catalyzing the C-prenylation of aromatic substrates. Recently, a new family of aromatic prenyltransferases was discovered in streptomycetes (1), Gram-positive soil bacteria that are prolific producers of antibiotics and other biologically active compounds (2). The members of this enzyme family show a new type of protein fold with a unique ␣-␤-␤-␣ architecture (3) and were therefore termed ABBA prenyltransferases (1). Only 13 members of this family can be identified by sequence similarity searches in the data base at present, and only four of them have been investigated biochemically (3-6). Up to now, only phenolic compounds have been identified as aromatic substrates of ABBA prenyltransferases. We now report the discovery of a new member of the ABBA prenyltransferase family, catalyzing the transfer of a dimethylallyl moiety to C-9 of 5,10-dihydrophenazine 1-carboxylate (dihydro-PCA).2 Streptomyces strains produce many of prenylated phenazines as natural products. For the first time, the present paper reports the identification of a prenyltr...

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“…Similarly PCR primers targeting aminoglycoside phosphotransferase genes (APH) were used to detect resistance genes associated to these biosynthetic pathways [26]. In a different approach the occurrence of isoprenoid biosynthesis pathways in actinomycetes was studied with PCR primers designed for the 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG CoA) [25]. For that reason, the combined data derived from the chemotaxonomic and molecular fingerprinting methods and the detection of biosynthetic systems such as PKS-I, PKS-II, NRPS, APH and HMG CoA have been applied to evaluate the diversity of this microbial community from a different perspective, in terms of the richness in these biosynthetic pathways.…”

Section: Introductionmentioning

confidence: 99%

Actinomycetes isolated from lichens: Evaluation of their diversity and detection of biosynthetic gene sequences

González

Ayuso-Sacido

Anderson

et al. 2005

FEMS Microbiology Ecology

164

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Actinomycetes, one of the major communities of the microbial population present in soil, can also be found inhabiting a wide diversity of ecological sources. We have explored the use of lichens as an alternative source for the isolation of novel actinomycetes. Lichens are symbiotic mixtures of fungi, green algae and/or cyanobacteria and whereas these symbiotic components have been extensively described, the microbial community inhabiting this niche has not been well characterized. We studied the diversity of the actinomycete population isolated from lichens collected in tropical areas from the Hawaii and Reunion islands and in cold areas from Alaska. The diversity of the microbial population was evaluated using fatty acid analysis and molecular fingerprinting. A PCR approach to screen the isolates for genes associated with secondary metabolite production was applied to evaluate the biosynthetic potential of these strains; profiles obtained for each isolate were compared to the antimicrobial activity exhibited by these isolates in laboratory conditions. Our results demonstrate that lichens represent an extremely rich reservoir for the isolation of a wide diversity of actinomycetes many of them representing still today a rich untapped source of secondary metabolites.

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Detection of Eubacterial 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductases from Natural Populations of Actinomycetes

Cited by 19 publications

References 24 publications

Hybrid isoprenoid secondary metabolite production in terrestrial and marine actinomycetes

Hybrid isoprenoid secondary metabolite production in terrestrial and marine actinomycetes

Aromatic Prenylation in Phenazine Biosynthesis

Actinomycetes isolated from lichens: Evaluation of their diversity and detection of biosynthetic gene sequences

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