Detection of Escherichia coli O157:H7 in imported meat products from Saudi Arabian ports in 2017

Alhadlaq, Meshari Ahmed; Mujallad, Mohammed I.; Alajel, Sulaiman M.

doi:10.1038/s41598-023-30486-2

Cited by 5 publications

(21 citation statements)

References 14 publications

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“…Our ndings were similar with a recent study conducted in 2017, by Saudi Food and Drug Authority (SFDA) that reported the presence of E. coli O157:H7 in 6.8% and 2.2% of the examined beef meat samples imported from India and Brazil, respectively. Moreover, the study concluded that the imported meat can serve as a carrier of E. coli O157:H7, which may lead to epidemics throughout regions of Kingdom of Saudi Arabia (Alhadlaq et al 2023).…”

Section: Discussionmentioning

confidence: 98%

Improved isolation and detection of shiga toxin-producing Escherichia coli O157 and non-O157 from retail imported frozen beef marketed in Saudi Arabia using immunomagnetic separation and multiplex-PCR

Almulhim,

Alomar,

Alhabib

et al. 2024

Preprint

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Shiga toxin-producing Escherichia coli (STEC) and E. coli O157:H7 are responsible for a wide range of foodborne outbreaks worldwide. Both E. coli O157 and non-O157 are causing life-threatening complications, such as hemolytic colitis and hemolytic uremic syndrome, which can result in kidney failure. This study was conducted to determine the presence of both STEC and E. coli O157 in imported frozen beef marketed in the Eastern Province of Saudi Arabia using immunomagnetic beads (IMB), CHROMagr O157 medium, and multiplex-PCR. A total of 201 frozen beef samples were purchased from different supermarkets in the Eastern Province of Saudi Arabia and enriched in tryptic soy broth (TSB). One-milliliter portions from enriched samples in TSB were mixed with immunomagnetic beads (IMB) coated with specific antibodies against E. coli O157 O antigen, separated beads with captured bacteria streaked on CHROMagar O157, and Sorbitol MacConkey (SMAC) agar. The extracted DNA template was examined for potential virulence gene markers (stx-1, stx-2, and eae) using multiplex-PCR. ERIC-PCR DNA fingerprinting was used to identify the clonal relationship among the strains isolated from frozen beef imported from different countries. Among 201 examined frozen beef samples, 88 (43.8%) and 106 (52.7%) were positive for E. coli and produced colorless and mauve colonies on SMAC agar and CHROMagar O157, respectively. Out of 298 isolates in total, 124 and 174 isolates of E. coli were isolated without and with IMB enrichment, respectively. The highest detection rate of virulence gene markers were found among isolates that had been isolated using IMB enrichment, where 25 (8.4%), 2 (0.7%) and 12 (4%) isolates tested positive for stx1, stx2, and eae genes respectively. The least number of virulence gene markers were found among isolates that were isolated without using IMB enrichment with a rate of 12 (4%) and 2 (0.7%) isolates testing positive for stx1 and eae genes, respectively. Among 42 isolates harboring potential virulence gene markers, 11 isolates were identified as E. coli O157 (stx1+/eae+ or stx2+/eae+). ERIC-PCR DNA fingerprinting was able to type the 42 identified isolates of E. coli O157 and E. coli non-O157 into 10 types with four identical related clusters and a genetic similarity rate above 90% homology from the identified isolates. This study confirmed that the imported frozen beef marketed in the Eastern Province of Saudi Arabia harbored virulence gene markers of E. coli O157. IMB and multiplex-PCR methods proved to be more reliable and adequate for detecting shiga toxin-producing E. coli O157 in imported frozen beef.

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Section: Discussionmentioning

confidence: 98%

Improved isolation and detection of shiga toxin-producing Escherichia coli O157 and non-O157 from retail imported frozen beef marketed in Saudi Arabia using immunomagnetic separation and multiplex-PCR

Almulhim,

Alomar,

Alhabib

et al. 2024

Preprint

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“…Amongst these, E. coli O157:H7 was detected respectively 6.80% and 2.20% of the tested beef meat samples . During enrichment, samples weighing 25 g selected for enrichment were placed in sterilised sample bags [152]. They were then homogenized with 225 mL of modified tryptone soya broth (mTSB) supplemented with novobiocin to obtain a ratio: mTSB + sample of 1/10 (mass to volume) [152].…”

Section: )mentioning

confidence: 99%

“…During enrichment, samples weighing 25 g selected for enrichment were placed in sterilised sample bags [152]. They were then homogenized with 225 mL of modified tryptone soya broth (mTSB) supplemented with novobiocin to obtain a ratio: mTSB + sample of 1/10 (mass to volume) [152]. The sample bags were massaged by hand and then incubated at 41.5° C for 12-18 h [1-152-167].…”

Section: )mentioning

confidence: 99%

“…The sample bags were massaged by hand and then incubated at 41.5° C for 12-18 h [1-152-167]. After incubation, the samples were subjected to immunomagnetic separation [152]. Subsequently, 50 μL of each sample was streaked out on pre-dried cefixime tellurite sorbitol MacConkey (CT-SMAC) agar plates using sterile loops to obtain many well-isolated colonies and incubated at 37°C for 18-24 h [152].…”

Section: )mentioning

confidence: 99%

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Pathogenic Escherichia coli (E. coli) food borne outbreak: Detection methods and controlling measures

Ravindra B. Malabadi,

Sadiya MR,

Kiran P. Kolkar

et al. 2024

Magna Sci. Adv. Res. Rev.

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Food-borne illnesses and diseases are major threats to human health. Common food borne pathogens include bacteria such as Escherichia coli Serogroup (O157), Bacillus cereus, Clostridium botulinum, Campylobacter sp., C. perfringens, some Listeria monocytogenes, Salmonella spp., Shigella spp., Staphylococcus aureus, and Vibrio spp. etc. Pathogenic E. coli infection usually causes severe diarrhea. Escherichia coli is the most common cause of acute urinary tract infections as well as urinary tract sepsis. Diarrheagenic E. coli pathotypes can be passed in the feces of humans and other animals. Safeguarding public health during mass gatherings is a big challenge. Despite the recent advances in food preservation techniques and food safety, significant disease outbreaks linked to food borne pathogens such as bacteria, fungi, and viruses still occur worldwide indicating that these pathogens still constitute significant risks to public health. However, at present the conventional method for E. coli detection requires several days, especially in cases where E. coli concentrations are low. Enrichment is a commonly used method for bacterial isolation to increase the cell counts of target bacteria. However, traditional culturing method is also a slow and laborious process requiring a series of steps and may require the use of adjunct methods (e.g. biochemical, serological, nucleic acid-based methods for conclusive identification) and can take up to a week for bacteria. The culture-based approaches coupled with other methods such as PCR, immunoassays, bacteriophages, NGS, Biosensors, and MALDI TOF MS are increasingly being used for the detection and identification of food borne pathogens. Nucleic acid-based methods such as Real-Time PCR and vPCR combined with sequencing approaches are more widely used than immunoassay and NGS-based approaches for pathogen detection. Nanobiotechnology is the latest approach for the detection of pathogens. Bacteriophages are one such unique biological entity that showed excellent host selectivity and have been actively used as recognition probes for pathogen detection. Antibiotics are efficient, powerful, and the most commonly used treatment against pathogenic E. coli. Other ways to treat or prevent E. coli contamination, such as probiotics, antimicrobial nanoparticles, and radiation treatment had been reported.

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“…Since 2000, the global economic cost of zoonotic outbreaks has exceeded USD 10 billion [ 5 ]. Zoonotic strains have caused a huge economic burden worldwide, examples include Listeria monocytogenes ( L. monocytogenes ) [ 6 ], Streptococcus suis ( S. suis ) [ 7 ], Staphylococcus aureus ( S. aureus ) [ 8 ], Salmonella enterica ( S. enterica ) [ 9 ] and Escherichia coli O157:H7 ( E. coli O157:H7 ) [ 10 ]. In 2022, the Ministry of Agriculture of the People’s Republic of China (PRC) confirmed that salmonellosis, swine streptococcosis and listeriosis are important zoonotic diseases [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Europium Nanoparticle-Based Lateral Flow Strip Biosensors Combined with Recombinase Polymerase Amplification for Simultaneous Detection of Five Zoonotic Foodborne Pathogens

Jin

Mei

et al. 2023

Biosensors

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The five recognized zoonotic foodborne pathogens, namely, Listeria monocytogenes, Staphylococcus aureus, Streptococcus suis, Salmonella enterica and Escherichia coli O157:H7, pose a major threat to global health and social–economic development. These pathogenic bacteria can cause human and animal diseases through foodborne transmission and environmental contamination. Rapid and sensitive detection for pathogens is particularly important for the effective prevention of zoonotic infections. In this study, rapid and visual europium nanoparticle (EuNP)-based lateral flow strip biosensors (LFSBs) combined with recombinase polymerase amplification (RPA) were developed for the simultaneous quantitative detection of five foodborne pathogenic bacteria. Multiple T lines were designed in a single test strip for increasing the detection throughput. After optimizing the key parameters, the single-tube amplified reaction was completed within 15 min at 37 °C. The fluorescent strip reader recorded the intensity signals from the lateral flow strip and converted the data into a T/C value for quantification measurement. The sensitivity of the quintuple RPA-EuNP-LFSBs reached a level of 101 CFU/mL. It also exhibited good specificity and there was no cross-reaction with 20 non-target pathogens. In artificial contamination experiments, the recovery rate of the quintuple RPA-EuNP-LFSBs was 90.6–101.6%, and the results were consistent with those of the culture method. In summary, the ultrasensitive bacterial LFSBs described in this study have the potential for widespread application in resource-poor areas. The study also provides insights in respect to multiple detection in the field.

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Detection of Escherichia coli O157:H7 in imported meat products from Saudi Arabian ports in 2017

Cited by 5 publications

References 14 publications

Improved isolation and detection of shiga toxin-producing Escherichia coli O157 and non-O157 from retail imported frozen beef marketed in Saudi Arabia using immunomagnetic separation and multiplex-PCR

Improved isolation and detection of shiga toxin-producing Escherichia coli O157 and non-O157 from retail imported frozen beef marketed in Saudi Arabia using immunomagnetic separation and multiplex-PCR

Pathogenic Escherichia coli (E. coli) food borne outbreak: Detection methods and controlling measures

Europium Nanoparticle-Based Lateral Flow Strip Biosensors Combined with Recombinase Polymerase Amplification for Simultaneous Detection of Five Zoonotic Foodborne Pathogens

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