Detection of Equine Herpesvirus-1 in the Fetal Membranes of Aborted Equine Fetuses by Immunohistochemical and In-situ Hybridization Techniques

Szeredi, L.; Aupperle, H.; Steiger, Katja

doi:10.1016/s0021-9975(03)00022-7

Cited by 17 publications

(17 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…IHC analysis showed the presence of EHV-1 antigen in tissues of aborted fetus. IHC analysis is an important diagnostic tool especially in cases suspected of EHV infection in which specific histopathological changes are not found (Szeredi et al, 2003). In the present study the viral etiology of the abortions outbreaks was confirmed based on the histological lesions along with the detection of antigens of EHV-1 by immunohistochemical analysis.…”

supporting

confidence: 54%

“…Additionally, the sequencing of the PCR products and BLAST results obtained with the consensus sequence (100% identity with multiple gB sequences available in the GenBank) confirmed the presence of EHV-1 DNA in the tissues analyzed. EHV-1 abortion was first diagnosed in Southern Brazil in 1994 by isolation of virus associated with the high prevalence of EHV-1 antibodies in the equine population (Weiblen et al, 1994). These authors suggested that abortions due to this virus are more frequent than reported as a consequence of absence of laboratory diagnosis.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Identification of equine herpesvirus type 1 as cause of abortion in mares in Southern Brazil

Estima-Silva

Riet-Correa

Coelho

et al. 2019

Arq. Bras. Med. Vet. Zootec.

View full text Add to dashboard Cite

show abstract

supporting

confidence: 54%

mentioning

confidence: 99%

Identification of equine herpesvirus type 1 as cause of abortion in mares in Southern Brazil

Estima-Silva

Riet-Correa

Coelho

et al. 2019

Arq. Bras. Med. Vet. Zootec.

View full text Add to dashboard Cite

show abstract

“…Immunohistochemistry and in situ hybridization are valuable for postmortem diagnosis. 7,23,24,26 Conventional PCR is a sensitive technique for EHV diagnosis and can distinguish between EHV-1 and EHV-4 infection. 12,20 When nested PCR is used it is possible to detect latent EHV infection.…”

Section: Introductionmentioning

confidence: 99%

Detection and Quantification of Equine Herpesvirus-1 Viremia and Nasal Shedding by Real-Time Polymerase Chain Reaction

et al. 2006

View full text Add to dashboard Cite

Equine herpesvirus-1 (EHV-1) infection is common in young horses throughout the world, resulting in respiratory disease, epidemic abortion, sporadic myelitis, or latent infections. To improve on conventional diagnostic tests for EHV-1, a real-time polymerase chain reaction (PCR) technique was developed, using primers and probes specific for the EHV-1 gB gene. Amplification efficiencies of 100% +/- 5% were obtained for DNA isolated from a plasmid, infected peripheral blood mononuclear cells (PBMCs), and nasal secretions from infected ponies. The dynamic range of the assay was 8 log10 dilutions, and the lower limit of detection was 6 DNA copies. Fifteen ponies, seronegative for EHV-1, were experimentally infected with EHV-1, and nasal samples were used to quantify shedding of virus by both virus isolation and real-time PCR analysis. Virus isolation identified nasal shedding of EHV-1 in 12/15 ponies on a total of 25 days; real-time PCR detected viral shedding in 15/15 ponies on 75 days. Viremia was quantified using PBMC DNA, subsequent to challenge infection in 3 additional ponies. Viremia was identified in 1/3 ponies on a single day by virus isolation; real-time PCR detected viremia in 3/3 ponies on 17 days. When real-time PCR was used to analyze PBMC DNA from 11 latently infected ponies (documented by nested PCR), EHV-1 was not detected. We conclude that real-time PCR is a sensitive and quantitative test for EHV-1 nasal shedding and viremia and provides a valuable tool for EHV-1 surveillance, diagnosis of clinical disease, and investigation of vaccine efficacy.

show abstract

“…Arzul et al (2002) observed also a positive signal in gonads and epithelial cells of gills in asymptomatic C. gigas adults by ISH. Protein detection in the epithelia were reported for equine herpesvirus 1 (EHV-1) (Easton et al, 2009;Szeredi et al, 2003) and human herpesvirus 8 (HHV-8) (Chagas et al, 2006). These viral proteins were also observed in cells involved in immune system, such as mononuclear cells (Ojok and Brown, 1996), lymphoid cells (Chagas et al, 2006), or monocytes (Szeredi et al, 2003).…”

Section: Discussionmentioning

confidence: 98%

In situ localization and tissue distribution of ostreid herpesvirus 1 proteins in infected Pacific oyster, Crassostrea gigas

Martenot

Segarra

Baillon

et al. 2016

Journal of Invertebrate Pathology

View full text Add to dashboard Cite

Immunohistochemistry (IHC) assays were conducted on paraffin sections from experimentally infected spat and unchallenged spat produced in hatchery to determine the tissue distribution of three viral proteins within the Pacific oyster, Crassostrea gigas. Polyclonal antibodies were produced from recombinant proteins corresponding to two putative membrane proteins and one putative apoptosis inhibitor encoded by ORF 25, 72, and 87, respectively. Results were then compared to those obtained by in situ hybridization performed on the same individuals, and showed a substantial agreement according to Landis and Koch numeric scale. Positive signals were mainly observed in connective tissue of gills, mantle, adductor muscle, heart, digestive gland, labial palps, and gonads of infected spat. Positive signals were also reported in digestive epithelia. However, few positive signals were also observed in healthy appearing oysters (unchallenged spat) and could be due to virus persistence after a primary infection. Cellular localization of staining seemed to be linked to the function of the viral protein targeted. A nucleus staining was preferentially observed with antibodies targeting the putative apoptosis inhibitor protein whereas a cytoplasmic localization was obtained using antibodies recognizing putative membrane proteins. The detection of viral proteins was often associated with histopathological changes previously reported during OsHV-1 infection by histology and transmission electron microscopy. Within the 6h after viral suspension injection, positive signals were almost at the maximal level with the three antibodies and all studied organs appeared infected at 28h post viral injection. Connective tissue appeared to be a privileged site for OsHV-1 replication even if positive signals were observed in the epithelium cells of different organs which may be interpreted as a hypothetical portal of entry or release for the virus. IHC constitutes a suited method for analyzing the early infection stages of OsHV-1 infection and a useful tool to investigate interactions between OsHV-1 and its host at a protein level.

show abstract

Detection of Equine Herpesvirus-1 in the Fetal Membranes of Aborted Equine Fetuses by Immunohistochemical and In-situ Hybridization Techniques

Cited by 17 publications

References 10 publications

Identification of equine herpesvirus type 1 as cause of abortion in mares in Southern Brazil

Identification of equine herpesvirus type 1 as cause of abortion in mares in Southern Brazil

Detection and Quantification of Equine Herpesvirus-1 Viremia and Nasal Shedding by Real-Time Polymerase Chain Reaction

In situ localization and tissue distribution of ostreid herpesvirus 1 proteins in infected Pacific oyster, Crassostrea gigas

Contact Info

Product

Resources

About