Detection of Epstein-Barr Virus in Invasive Breast Cancers

Bonnet, Mathilde; Guinebretière, Jean‐Marc; Kremmer, Elisabeth; Grunewald, Virginie; Benhamou, E; Contesso, G.; Joab, Irène

doi:10.1093/jnci/91.16.1376

Cited by 286 publications

(282 citation statements)

References 22 publications

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“…In our study, the presence of EBV in tumoral lymphoplasmocytic infiltration can be ruled out. First, ISH demonstrated no specific labelling of the stroma and the infiltrating lymphoplasmocytic cells near the positive tumoral epithelial clusters; secondly, LCM combined with Q-PCR also showed that EBV localization was restricted to some tumour epithelial cell clusters; thirdly, the tumours found to be EBV negative using BamHIC PCR included samples with low, moderate, and high degrees of lymphoplasmocytic infiltration; finally, EBV was not detected in normal tissues adjacent to breast cancers and benign breast tumours, in agreement with previous studies (Labrecque et al, 1995;Bonnet et al, 1999).…”

Section: Discussionsupporting

confidence: 88%

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Frequency and genome load of Epstein–Barr virus in 509 breast cancers from different geographical areas*

et al. 2001

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Since the few data exploring a possible association between Epstein–Barr virus (EBV) and breast cancer are conflicting, we investigated this association together with the influences of geographical areas. 509 breast cancers were sampled from areas with varying risks of nasopharynx carcinoma (NPC) such as North Africa (Algeria and Tunisia, high-risk area); southern France (Marseille, intermediate-risk area); and northern Europe (northern France, the Netherlands and Denmark; low-risk areas). Polymerase chain reaction (PCR) of a subregion of EBV BamHIC encoding the EBERs demonstrated that 31.8% of the tumours contained the viral genome. No significant differences were observed among the geographical areas. However, positive samples showed higher loads of the EBV genome in the NPC high- and intermediate-risk areas than in the low-risk areas. EBV type 1 was the dominant strain. In situ hybridization studies using a 35 S-labelled riboprobe for EBER1 and a laser capture microdissection, combined with quantitative PCR, showed that EBV localization was restricted to some tumour epithelial cell clusters. EBV could not be detected in the stroma. Considering the whole population covered, the presence of the EBV genome was not correlated with age, menopausal status, tumour, size, nodal status or histological grade. © 2001 Cancer Research Campaign http://www. bjcancer.com

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Section: Discussionsupporting

confidence: 88%

“…In Marseille, there was however a trend toward a positive association between EBV and grade, as reported by Bonnet et al (1999). High rates of positive tumours were observed in young Algerian patients and in inflammatory samples.…”

Section: Discussionsupporting

confidence: 59%

Frequency and genome load of Epstein–Barr virus in 509 breast cancers from different geographical areas*

et al. 2001

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“…One study reported multiple and bilateral fibroadenomas in immunosuppressed patients receiving cyclosporin A (17). Isolated studies have investigated the EBV/breast cancer association with contradictory results (2,18,19). This is the first study to investigate the role of EBV in rapidly growing breast tumors in immunocompromised patients.…”

Section: Discussionmentioning

confidence: 99%

“…Despite the widespread prevalence of EBV infection, EBV has been directly implicated in the development of only two malignancies observed in relatively restricted geographic areas: Burkitt's Lymphoma in East Africa and nasopharyngeal carcinoma in Southern China. It has also been closely associated to Hodgkin's Disease, thymic lymphoepithelioma, opportunistic B-cell lymphomas, pleural B-cell lymphomas or pyothorax associated B-cell lymphomas, opportunistic T-cell and NK-cell lymphomas in immunodeficiencies, primary nasal T-cell and NK-cell lymphomas, gastric carcinoma, and most recently, invasive breast carcinoma (1)(2)(3)(4)(5)(6)(7)(8)(9).…”

Section: Epstein-barr Virus (Ebv) Is a Common Humanmentioning

confidence: 99%

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Detection of Epstein-Barr Virus in Rapidly Growing Fibroadenomas of the Breast in Immunosuppressed Hosts

et al. 2002

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Fibroadenomas are the most common benign tumors of the female breast and are associated with a slight increase in the risk of subsequent breast cancer. Multiple fibroadenomas have been described in patients after renal transplantation and are thought to be secondary to drug-related growth stimulation. Epstein-Barr virus (EBV) has been detected in many neoplasms, including breast cancer. We set out to investigate whether EBV plays a role in the development of rapidly growing fibroadenomas in immunocompromised patients. We studied 19 fibroadenomas and one invasive ductal carcinoma that developed after organ transplantation or treatment for lupus erythematosus. As a control group we included 11 fibroadenomas from non-immunocompromised patients. DNA was amplified using polymerase chain reaction (PCR) of the EBV-encoded small RNA (EBER-2) DNA sequence. EBV latent membrane protein 1 (LMP-1) transcripts were amplified using reverse transcription (RT) PCR. Immunohistochemical (IHC) staining for LMP-1 protein was performed. A total of 9 out of 20 tumors (45%) were concordantly positive by PCR and IHC. IHC stained exclusively the epithelial cells. All the fibroadenomas in non-immunocompromised patients were negative for LMP-1 (Fisher's exact test P ‫؍‬ .0006). These data suggest that EBV is associated with fibroadenomas in this immunosuppressed population and that the infection is specifically localized to epithelial cells. This is the first study suggesting a role for EBV in the pathogenesis of fibroadenomas.

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Heterogeneity of HLA and EBER expression in epstein-barr virus-associated nasopharyngeal carcinoma

et al. 2000

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Detection of Epstein-Barr Virus in Invasive Breast Cancers

Abstract: Our results demonstrated the presence of the EBV genome in a large subset of breast cancers. The virus was restricted to tumor cells and was more frequently associated with the most aggressive tumors. EBV may be a cofactor in the development of some breast cancers.

Cited by 286 publications

References 22 publications

Frequency and genome load of Epstein–Barr virus in 509 breast cancers from different geographical areas*

Frequency and genome load of Epstein–Barr virus in 509 breast cancers from different geographical areas*

Detection of Epstein-Barr Virus in Rapidly Growing Fibroadenomas of the Breast in Immunosuppressed Hosts

Heterogeneity of HLA and EBER expression in epstein-barr virus-associated nasopharyngeal carcinoma

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