Detection of epidermodysplasia verruciformis-associated human papillomavirus DNA in nongenital seborrhoeic keratosis

Yh, Li; Chen, G; Xp, Dong; Hd, Chen

doi:10.1111/j.1365-2133.2004.06244.x

Cited by 44 publications

(58 citation statements)

References 31 publications

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“…In addition, other investigators using similar methods have found comparable results: HPV DNA has been detected in 4% to 35% of normal-appearing skin samples. [53][54][55][56][57][58] Coinfection with EV and GM HPV genotypes was found in both EN types (two HPV DNA [EV and GM] and 3 HPV DNA [two EV and one GM]). For NS, 59% harbored EV HPV DNA, and 50% had GM HPV DNA.…”

Section: Hpv Dna Is Frequently Detected In Nsmentioning

confidence: 99%

See 1 more Smart Citation

Epidermodysplasia verruciformis–associated and genital-mucosal high-risk human papillomavirus DNA are prevalent in nevus sebaceus of Jadassohn

Carlson

Cribier

Nuovo

et al. 2008

Journal of the American Academy of Dermatology

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Section: Hpv Dna Is Frequently Detected In Nsmentioning

confidence: 99%

“…This cocktail probe detects the following 12 genotypes: 16,18,31,33,35,39,45,51,52,56,58, and 66. The xenograft control slide, HPV 3 in one system control slides (Ventana Medical Systems Inc), was run for positive and negative controls.…”

Section: Sequencingmentioning

confidence: 99%

Epidermodysplasia verruciformis–associated and genital-mucosal high-risk human papillomavirus DNA are prevalent in nevus sebaceus of Jadassohn

Carlson

Cribier

Nuovo

et al. 2008

Journal of the American Academy of Dermatology

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“…7 These HPV types are detectable in skin carcinomas, as well as in control specimens of healthy skin from the general population. [8][9][10][11][12] There are more than 100 different HPV types that have been completely sequenced. 7 In addition, there appears to exist about 100 additional putative HPV types, with sequence information so far available only from PCR amplimers.…”

mentioning

confidence: 99%

Subtype HPV38b[FA125] demonstrates heterogeneity of human papillomavirus type 38

Hazard

Eliasson

Dillner

et al. 2006

Intl Journal of Cancer

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The human papillomaviruses (HPVs) exist as more than 100 distinct types. While variants of HPV are common, only few HPV subtypes have been reported. HPV type 38 has been proposed to be associated with nonmelanoma skin cancer (NMSC), with reported prevalences of up to 55%. A subtype of HPV38 was cloned, completely sequenced and found to have a 96% sequence similarity to prototype HPV38 in the L1 open reading frame. The presence of prototype HPV38 and HPV38b[FA125] was examined in paired biopsies of tape-stripped skin lesions and healthy skin from 269 immunocompetent patients by real-time PCR. Prototype HPV38 and HPV38b[FA125] were present in seven (3%) and five (2%) lesions, respectively, in viral loads ranging from one copy per 150 cells to one copy per 70,000 cells. In summary, we found that HPV38 is heterogeneous and is one of so far only few HPVs that contain subtypes. The heterogeneity needs to be considered in studies of the biology of this virus. ' 2006 Wiley-Liss, Inc.Key words: HPV; subtype; nonmelanoma skin cancer; multiplyprimed rolling circle amplification Nonmelanoma skin cancers (NMSC) are the most prevalent malignancies in the Caucasian population 1 and an increase in prevalence has been seen in European countries during the last few years. 2 NMSC comprises basal cell carcinoma (BCC) and squamous cell carcinoma (SCC). BCC has a relatively good prognosis, while SCC accounts for up to 20% of all deaths from skin cancer. 3 NMSC are often found on sun-exposed sites in patients with the rare inherited genetic disease epidermodysplasia verruciformis (EV). 4 These patients are highly susceptible to human papillomavirus (HPV) infections 5,6 mainly by the Beta-papillomaviruses, a phylogenetic subgroup of cutaneous HPVs (e.g., HPV5 and HPV8). 7 These HPV types are detectable in skin carcinomas, as well as in control specimens of healthy skin from the general population. [8][9][10][11][12] There are more than 100 different HPV types that have been completely sequenced. 7 In addition, there appears to exist about 100 additional putative HPV types, with sequence information so far available only from PCR amplimers. 8,[13][14][15][16] Variants of HPV types contain more than 98% sequence homology in the L1 open reading frame (ORF). 7 Variants is a common finding that appears to associate with ethnic groups and sometimes even with biologic behavior of the virus. 17,18 By contrast, subtypes containing 90-98% sequence homology in the L1 region are very rare, with HPV 68a/HPV68b, HPV46, HPV55 and HPV64 (now known to be subtypes, originally thought to be different viruses) being the only fully characterized examples. 7,17 Although the reasons for this are not known, existence of subtypes has profound implications for design of virus detection systems and for understanding of the biology of the viruses.Epidemiological studies have established a causal association between UV-radiation and NMSC, 3 but the role of HPV in these cancers remains to be elucidated.A recent study 19 has demonstrated that the early proteins E...

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“…Applying nested PCR with consensus primers revealed more positive results for EV HPV DNA in non-genital SK compared to healthy controls. [13] In nested PCR double genome amplification is used and the significant difference in positive results from two steps PCR genome amplification, strongly suggested a very low copy number for EV HPV DNA. [13] Gushi et al .…”

Section: Discussionmentioning

confidence: 99%

“…[13] In nested PCR double genome amplification is used and the significant difference in positive results from two steps PCR genome amplification, strongly suggested a very low copy number for EV HPV DNA. [13] Gushi et al . study detected mucosal HPV-18, 81, 6 in most of their samples.…”

Section: Discussionmentioning

confidence: 99%

Human papillomavirus deoxyribonucleic acid may not be detected in non-genital benign papillomatous skin lesions by polymerase chain reaction

et al. 2014

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Background:Papillomatosis is a known histopathologic pattern usually seen in human papillomavirus (HPV) infection and verruca vulgaris is the typical example. This pattern is also detected in some other benign cutaneous lesions such as nevus sebaceous (NS), seborrheic keratosis (SK), trichilemmoma (TL) and inverted follicular keratosis (IFK). The association between papillomatous lesions and HPV infection is questionable.Objective:The objective of this study was to investigate the presence of HPV deoxyribonucleic acid (DNA) in non-genital benign papillomatous skin lesions (NS, SK, TL and IFK) by polymerase chain reaction (PCR).Materials and Methods:A total of 100 specimens of non-genital NS, SK, TL and IFK were retrieved from archives of Dermatopathology Department of Razi Hospital, between 2003 and 2010. The conventional PCR using consensus GP5+/GP6+ primer and hydroxymethylbilane synthase gene as inner control was performed.Results:PCR for HPV DNA revealed no positive results in any of 28 seborrheic keratosis (SK), 28 nevus sebaceous (NS), 28 inverted follicular keratosis (IFK) and 13 trichilemmoma (TL) studied specimens.Conclusion:Papillomatosis is usually a characteristic pattern of HPV infection. However, we found no association between HPV infection and non-genital benign papillomatous lesions.

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Detection of epidermodysplasia verruciformis-associated human papillomavirus DNA in nongenital seborrhoeic keratosis

Abstract: This study provides some evidence that EV-associated HPVs might play a part in the pathogenesis of nongenital SK.

Cited by 44 publications

References 31 publications

Epidermodysplasia verruciformis–associated and genital-mucosal high-risk human papillomavirus DNA are prevalent in nevus sebaceus of Jadassohn

Epidermodysplasia verruciformis–associated and genital-mucosal high-risk human papillomavirus DNA are prevalent in nevus sebaceus of Jadassohn

Subtype HPV38b[FA125] demonstrates heterogeneity of human papillomavirus type 38

Human papillomavirus deoxyribonucleic acid may not be detected in non-genital benign papillomatous skin lesions by polymerase chain reaction

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