Detection of epidermal growth factor receptor (EGFR) mutations in non-small cell lung cancer (NSCLC) using a fully automated system with a nano-scale engineered biomagnetite

Maruyama, Katsuhisa; Takeyama, Haruko; Mori, Tetsushi; Ohshima, Keiichi; Ogura, Shun; Mochizuki, Toru; Matsunaga, Tadashi

doi:10.1016/j.bios.2006.11.018

Cited by 17 publications

(12 citation statements)

References 22 publications

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“…Leucocytes, Bacillus spp. spores, CD34 + cells, several cancer cells including Hela cells, Escherichia coli, human immunodeficiency virus (HIV) have been separated by magnetic cell separation [24,26,[133][134][135][136][137][138][139][140]. Recently Chen et al used titanium-coated magnetic iron oxide nanoparticles (Fe 3 O 4 @TiO 2 NPs) as affinity probes to concentrate and characterize pathogenic bacteria.…”

Section: Biomolecules and Cellsmentioning

confidence: 98%

Magnetic solids in analytical chemistry: A review

Aguilar-Arteaga

Rodríguez

Barrado

2010

Analytica Chimica Acta

386

148

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Section: Biomolecules and Cellsmentioning

confidence: 98%

Magnetic solids in analytical chemistry: A review

Aguilar-Arteaga

Rodríguez

Barrado

2010

Analytica Chimica Acta

386

148

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“…The method comprises PCR amplification of the target gene region by biotinylated primers, recovery of the amplified PCR products by streptavidinimmobilized BacMPs (SA-BacMPs), denaturation of duplex DNA, magnetic separation of SA-BacMPs with the single-stranded target DNA, hybridization with fluorescence-labelled probes and, finally, detection. SNPs of aldehyde dehydrogenase-2 (ALDH2; Maruyama et al 2004), epidermal growth factor receptor (EGFR; Maruyama et al 2007) and transforming growth factor beta-1 (TGF-b1; Matsunaga et al 2007b) genes have been successfully detected. Certain encumbrances are encountered in the SNP discrimination of each target gene.…”

Section: High-throughput Single Nucleotide Polymorphism Detection Systemmentioning

confidence: 99%

Formation of magnetite by bacteria and its application

Arakaki

Nakazawa

Nemoto

et al. 2008

J. R. Soc. Interface.

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219

176

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Magnetic particles offer high technological potential since they can be conveniently collected with an external magnetic field. Magnetotactic bacteria synthesize bacterial magnetic particles (BacMPs) with well-controlled size and morphology. BacMPs are individually covered with thin organic membrane, which confers high and even dispersion in aqueous solutions compared with artificial magnetites, making them ideal biotechnological materials. Recent molecular studies including genome sequence, mutagenesis, gene expression and proteome analyses indicated a number of genes and proteins which play important roles for BacMP biomineralization. Some of the genes and proteins identified from these studies have allowed us to express functional proteins efficiently onto BacMPs, through genetic engineering, permitting the preservation of the protein activity, leading to a simple preparation of functional protein-magnetic particle complexes. They were applicable to high-sensitivity immunoassay, drug screening and cell separation. Furthermore, fully automated single nucleotide polymorphism discrimination and DNA recovery systems have been developed to use these functionalized BacMPs. The nano-sized fine magnetic particles offer vast potential in new nano-techniques.

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“…Maruyama et al (2004) developed a protocol based on DNA thermal dissociation curve analysis for an automated system with magnetosomes by developing a new method for avoiding light scattering caused by nanometer-size particles when using commercially-available fluorescent dyes such as FITC, Cy3, and Cy5 for labeling chromophores. SNPs of aldehyde dehydrogenase-2 (Maruyama et al 2004), epidermal growth factor receptor (Maruyama 2007) and transforming growth factor b-1 (Ota et al 2003;Matsunaga et al 2007) genes have been detected. In another study, a semi-automated SNPs detection system, based on thermal-dissociation-curve-analysis and allele-specific oligonucleotide hybridization using magnetosomes was developed by Matsunaga et al (2007).…”

Section: Single Nucleotide Polymorphisms (Snps)mentioning

confidence: 97%

Magnetotactic bacteria for cancer therapy

Mathuriya

2014

Biotechnol Lett

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Cancer is characterized by anomalous cell growth. Conventional therapies face many challenges and hence alternative treatment methods are in great demand. In addition, nature offers the best inspiration and recently many therapies of natural origin have proved multi-targeted, multi-staged, and a multi-component mode of action against cancer. Magnetotactic bacteria and magnetosomes-based treatment methods are among them. Present paper reviews various routes by which magnetotactic bacteria and magnetosomes contribute to cancer therapy.

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Detection of epidermal growth factor receptor (EGFR) mutations in non-small cell lung cancer (NSCLC) using a fully automated system with a nano-scale engineered biomagnetite

Cited by 17 publications

References 22 publications

Magnetic solids in analytical chemistry: A review

Magnetic solids in analytical chemistry: A review

Formation of magnetite by bacteria and its application

Magnetotactic bacteria for cancer therapy

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