Detection of enzootic pneumonia in pig herds using an enzyme-linked immunosorbent assay in sow colostrum

Levonen, K.

doi:10.1016/0034-5288(94)90205-4

Cited by 11 publications

(10 citation statements)

References 3 publications

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“…When the anti-PMT ELISA is regarded as a herd test, the presence of animals with high and low antibody titers indicates that the herd is infected with P. multocida. Similar to many other serologic tests for swine respiratory infections, the ELISA for PMT antibodies is not sensitive enough to classify a single animal into a positive or negative category 14,17,19 but can be used to screen infected herds.…”

Section: Discussionmentioning

confidence: 99%

Detection of Toxigenic Pasteurella Multocida Infections in Swine Herds by Assaying Antibodies in Sow Colostrum

Levonen¹,

Frandsen²,

Seppänen³

et al. 1996

J VET Diagn Invest

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Abstract. Toxigenic Pasteurella multocida is the causative agent of progressive atrophic rhinitis (PAR), a serious respiratory infection of swine. Diagnosis of the disease has hitherto been based on clinical signs, pathologic findings, and subsequent isolation of the agent. The best Finnish pig breeding herds participating in the Finnish Pig Health Scheme have been surveyed for PAR since 1963, and the disease has been eradicated from these herds. In this study, a total of 5,650 colostrum samples from 188 Finnish Pig Health Scheme herds were analyzed with a new serologic screening method: an enzyme-linked immunosorbent assay (ELISA) able to detect antibodies to the toxin of P. multocida (PMT). Although the herds had been continuously controlled for PAR, 1 herd with PMT antibodies was found. The positive reactions in the ELISA were confirmed by isolating the causative organism. The origin of the infection also appeared to be obvious. The serologic ELISA is a suitable method for the detection and screening of toxigenic P. multocida-infected pig herds.

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Section: Discussionmentioning

confidence: 99%

Detection of Toxigenic Pasteurella Multocida Infections in Swine Herds by Assaying Antibodies in Sow Colostrum

Levonen¹,

Frandsen²,

Seppänen³

et al. 1996

J VET Diagn Invest

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“…Serotype 2 is also the most prevalent in Sweden, but it seldom causes acute epidemics (BESKOW et al, 1989). The environmental conditions on the Finnish breeding farms are very good, and Mycoplasma hyopneumoniae infections are rare (LEVONEN, 1994), which may explain the lack of acute infections caused by A . pleuropneumoniae serotype 2 (ROSS, 1990 3 01 Y LEVONEN, VEIJALAINEN and SEWANEN serotype-2 antibodies.…”

Section: The Herdsmentioning

confidence: 99%

Actinobacillus pleuropneumoniae Serotype‐2 Antibodies in Sow Colostrum in Finnish Pig‐health‐scheme Herds

Levonen

Veijalainen

Seppänen

1994

Journal of Veterinary Medicine, Series B

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A direct ELISA with phenol-extracted antigen for Actinobacilluspleuropneumoniae serotype 2 was developed. The test was specific when tested with rabbit antisera prepared against different A. pleuropneumoniae serotypes. It had better-than-moderate repeatability and it made a clear distinction between positive and negative samples.A total of 5477 colostrum samples from breeding sows from herds participating in the Finnish Pig-health Scheme were tested using the ELISA test. A total of 1307 positive samples were found in 129 out of 154 herds, thus indicating that most of the disease-control herds in Finland are infected with A. pleuropneumoniae serotype 2. These infections were almost entirely subclinical.::. Corresponding author U.S.

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“…7 As reported earlier, the ELISA was more sensitive for colostrum than for the serum samples. 5 Other researchers have reported an experimental infection of enzootic pneumonia in which 16 of 24 sows were classified positive on the basis of colostrum samples but only 4 sows were positive in tests on parallel serum samples. 11 However, excessive freezing and thawing of colostrum samples should be avoided because of the decreased positive reaction.…”

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“…5,6 At present, all the elite breeding herds are involved in the program. Suspect samples and cases where only a single sow in a herd has had antibodies have given rise to problems during the surveillance of the herds.…”

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confidence: 99%

“…Earlier studies have shown that these single positive reactions can be false positive, or a newly purchased animal that is mycoplasma negative but antibody positive, or the first signs of the start of an enzootic pneumonia infection. 5 The false-positive category mainly consists of contaminated samples that react positively during the test or can resemble ''singleton reactors,'' where an individual shows an incorrect antigenantibody reaction (Dr. Terpstra and Dr. E. Brocchi, personal communication). The falsely functioning antibodies of a singleton reactor are a problem with several other ELISA kits.…”

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Comparison of Two Commercial Enzyme-Linked Immunosorbent Assays for the Detection of Antibodies against Mycoplasma Hyopneumoniae and Correlation with Herd Status

Levonen¹,

Sihvo²,

Veijalainen³

1999

J VET Diagn Invest

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Detection of enzootic pneumonia in pig herds using an enzyme-linked immunosorbent assay in sow colostrum

Cited by 11 publications

References 3 publications

Detection of Toxigenic Pasteurella Multocida Infections in Swine Herds by Assaying Antibodies in Sow Colostrum

Detection of Toxigenic Pasteurella Multocida Infections in Swine Herds by Assaying Antibodies in Sow Colostrum

Actinobacillus pleuropneumoniae Serotype‐2 Antibodies in Sow Colostrum in Finnish Pig‐health‐scheme Herds

Comparison of Two Commercial Enzyme-Linked Immunosorbent Assays for the Detection of Antibodies against Mycoplasma Hyopneumoniae and Correlation with Herd Status

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