Detection of Enterovirus by Polymerase Chain Reaction and Culture in Cerebrospinal Fluid of Children with Transient Neurologic Complications Associated with Acute Febrile Illness

Honzumi, Ken; Suzuki, Hitoshi

doi:10.1093/infdis/175.3.700

Cited by 22 publications

(13 citation statements)

References 11 publications

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“…The procedure for nested PCR was described previously. 2 In brief, RNA was extracted from 250 L of sample. After RNA extraction, complementary DNA was synthesized from the resuspended RNA using 2.5 U of Moloney murine leukemia virus reverse transcriptase.…”

Section: Pcr For Detection Of the Enteroviral Genomementioning

confidence: 99%

“…2 Of the 18 patients with seizures that fulfilled diagnostic criteria for simple febrile seizures, 5 had positive results in their CSF as determined by enteroviral polymerase chain reaction (PCR). The present study was designed to clarify the relationship between enteroviral infection in the CNS and febrile seizures and to identify the enteroviruses that relate to the febrile seizures.…”

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confidence: 99%

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Association of Nonpolio Enteroviral Infection in the Central Nervous System of Children With Febrile Seizures

et al. 2001

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ABSTRACT. Objective. To clarify the relationship between enteroviral infection and febrile seizures.Study Design. Cerebrospinal fluid (CSF), serum, throat swab, and rectal swab samples were collected for virologic examination from 67 children with febrile seizures from April 1997 to March 1999. Those samples were examined for the presence of enterovirus using cell culture and 2 polymerase chain reaction (PCR) methods.Results. No enterovirus was isolated from cell culture of CSF, throat swab, or rectal swab samples. All samples were screened for the presence of enteroviral sequences using a sensitive PCR method (PCR-Fukushima). We obtained positive results from 14 of 67 CSF samples, 10 of 62 serum samples, 12 of 64 throat swab samples, and 13 of 64 rectal swab samples. Of 21 patients in whom febrile seizures had developed during the summer months (June through August), 13 (61.9%) had positive PCR results in the CSF. Forty-seven of the 49 samples with a positive result using PCR-Fukushima were reexamined independently for the presence of the enteroviral genome using another PCR method (PCR-Mitsubishi). PCR-Mitsubishi had slightly lower sensitivity than PCR-Fukushima but identified genotypes of enterovirus by subsequent sequence analysis of the PCR products. The presence of the enteroviral genome was confirmed in 39 of the samples (83.0%). In 8 of the 9 enteroviruses detected in the CSF and/or serum samples using PCR-Mitsubishi, the genotypes were identified as coxsackieviruses group A, which are usually difficult to isolate using cell culture methods.Conclusions. These findings proved that the causative agents of febrile illness associated with seizures in summer were primarily enteroviruses, especially coxsackieviruses group A, and that febrile seizures might be caused by enteroviral infection in the central nervous system. Pediatrics 2001;107(1). URL: http://www. pediatrics.org/cgi/content/full/107/1/e12; enterovirus, coxsackievirus group A, febrile seizures, polymerase chain reaction.ABBREVIATIONS. CNS, central nervous system; CSF, cerebrospinal fluid; PCR, polymerase chain reaction; bp, base pair. E nteroviruses-polioviruses, echoviruses, coxsackieviruses group A and group B, and enteroviruses-are responsible for significant and frequent human illness, including various neurologic manifestations. The most common neurologic illness is aseptic meningitis. Encephalitis and other manifestations, such as paralysis, Guillain-Barré syndrome, transverse myelitis, cerebellar ataxia, and peripheral neuritis, also occur. 1 The association of enteroviral infection in the central nervous system (CNS) with febrile seizures, however, has not been demonstrated. In general, the causative agent of the infection is not detected in the CNS of patients with febrile seizures using standard techniques.We previously reported that the enteroviral genome was detected in cerebrospinal fluid (CSF) samples collected from some children with transient neurologic complications, such as seizures and altered states of consciousness associated ...

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Section: Pcr For Detection Of the Enteroviral Genomementioning

confidence: 99%

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confidence: 99%

Association of Nonpolio Enteroviral Infection in the Central Nervous System of Children With Febrile Seizures

et al. 2001

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“…However, CSF specimens may not be the only optimal choice for the detection of EV71 by PCR or VC (26). Hosoya et al (27) detected EV using CSF samples from patients with CNS infections by PCR and VC and demonstrated a higher sensitivity by PCR, with 39.1z EV infections being detected by PCR and only 8.7z by VC. Singh et al (28) detected EV71 by RT-PCR and found sensitivity rates of 71z and 53z for pan-EV and EV71, respectively.…”

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confidence: 99%

Comparison of the Detection Rates of RT-PCR and Virus Culture Using a Combination of Specimens from Multiple Sites for Enterovirus-Associated Encephalomyelitis during Enterovirus 71 Epidemic

Tsai

Lin

et al. 2014

Jpn J Infect Dis

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SUMMARY: Enterovirus (EV) 71 may cause severe neurological illness in the pediatric population. The present study aimed to compare the detection rates of reverse transcription-polymerase chain reaction (RT-PCR) with pan-EV/EV71 type-specific primers and virus culture (VC) for the identification of EV and EV71 using specimens from multiple sites. In total, specimens from throat/rectal swabs, cerebrospinal fluid (CSF), and blood from 66 patients diagnosed with EV encephalomyelitis were subjected to both RT-PCR and VC for detecting the presence of pan-EV and EV71. The results revealed that the positive RT-PCR rate was higher in throat swabs (60.6z) and rectum swabs (50.0z) than in CSF (16.7z) and blood (15.6z). The same trend was also observed in case of VC: throat swabs (22.7z), rectum swabs (10.6z), and blood (3.0z). The detection rate of EV encephalomyelitis by RT-PCR was 90.9z for all samples, 63.6z of which were subtyped as EV71. The detection rates of RT-PCR were superior to those of VC, and identification using specimens from throat/rectal swabs yielded higher positive results. These findings may help physicians to identify the etiologies at an early stage during EV71 epidemics and to make emergent medical decisions for minimizing patient morbidity and mortality.

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“…We therefore attempted to detect enteroviral RNA in renal biopsies from patients with IgAN, using PCR. The PCR method we used could detect almost all prevalent enteroviruses in Japan, with a sensitivity a thousand times that of the virus isolation method [15].…”

Section: Discussionmentioning

confidence: 99%

“…Detection of enteroviral RNA by PCR PCR for enteroviral RNA amplification was performed as has been previously described [15,16]. After RNA extraction, cDNA was synthesized from the extracted RNA.…”

Section: Rna Extractionmentioning

confidence: 99%

Detection of enteroviruses in renal biopsies from patients with immunoglobulin A nephropathy

et al. 2005

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Viruses have been suspected to be one of the causes of IgA nephropathy (IgAN). Recent studies have detected viruses in renal tissues of patients with IgAN. Enteroviruses have been reported as pathogenic agents in some renal diseases. We previously reported that group B coxsackieviruses cause pathological changes in experimentally infected mouse kidney. The aim of the present study was to examine the participation of enteroviruses in the pathogenesis of renal diseases including IgAN. Renal biopsies of ten patients with IgAN (group 1) and of 19 patients with non-IgAN renal disease (group 2) were analyzed by polymerase chain reaction (PCR) for the presence of enteroviral RNA. Positive PCR results were obtained for three patients (30%) of group 1. We confirmed by sequencing that the positive PCR products were derived from strains of enteroviruses. One of these three patients also had a positive result for lymphocytes from peripheral blood. In contrast, enteroviral RNA was detected in none of the 19 patients of group 2. The incidence of enteroviral RNA detection in patients of group 1 was higher than that in group 2 (P<0.05). Our findings suggest that enteroviral infection may have the possibility of becoming one of the factors involved in the mechanism of onset or evolution of IgAN.

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Detection of Enterovirus by Polymerase Chain Reaction and Culture in Cerebrospinal Fluid of Children with Transient Neurologic Complications Associated with Acute Febrile Illness

Cited by 22 publications

References 11 publications

Association of Nonpolio Enteroviral Infection in the Central Nervous System of Children With Febrile Seizures

Association of Nonpolio Enteroviral Infection in the Central Nervous System of Children With Febrile Seizures

Comparison of the Detection Rates of RT-PCR and Virus Culture Using a Combination of Specimens from Multiple Sites for Enterovirus-Associated Encephalomyelitis during Enterovirus 71 Epidemic

Detection of enteroviruses in renal biopsies from patients with immunoglobulin A nephropathy

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