Detection of enterotoxins and genotyping of Staphylococcus aureus strains isolated from Isfahan Educational Hospital, Iran

Abstract

SummaryBackground and aims: Staphylococcus aureus is known as one of the most important nosocomial pathogens, which may lead to several infections. The aim of this study was determining the enterotoxins A, C, and TSST-1 and molecular characterization of S. aureus strains with PFGE and MLST typing methods. Materials and methods:In the present study during the sixmonths sampling, fifty S. aureus strains were isolated from patients admitted to Al-Zahra university hospital. Antimicrobial susceptibility testing, Multiplex PCR for detection of enterotoxin A, C and TSST-1, pulse field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) were used for molecular typing.Results: In antibiogram the highest and lowest percentage of resistance was belonged to tetracycline and rifampin respectively. Multiplex PCR indicated that 30% of the strains harbored sea and 34% harbored sec genes. However, only 4% of our collected isolates had tsst gene. In PFGE method analysis on all S. aureus strains, a total of 19 different patterns were identified. Nine various sequence types in 27 selected S. aureus isolates were identified by MLST.Conclusions: Present study indicates a possible higher variability among our S. aureus strains by two different molecular typing methods; nevertheless four main common types (CT1, CT7, CT9, and CT11) with at least one toxin genes were determined.

Genetic Identification of Methicillin-Resistant Staphylococcus aureus Nasal Carriage and Its Antibiogram among Kidney Dialysis Patients at a Tertiary Care Hospital in AL-Karak, Jordan

“…Many studies used these genes to recognize the MRSA. Te primers and probes in the Xpert SA Nasal Complete assay real-time PCR and RT-PCR enabled to detect specifc sequences for the staphylococcal protein A (spa), the gene for methicillin/oxacillin resistance (mecA), and the staphylococcal cassette chromosome (SCCmec) inserted into the SA chromosomal attB site [36][37][38][39].…”

Molecular Epidemiology and Surveillance Program in Iran: Present Status and Future Prospect

“…Furthermore, they cannot improve the health criteria or prediction of any epidemic disease. [27][28][29][30][31] Another problem is the main goal of the molecular epidemiology. As previously discussed, the aim of the type identification is to find out the source of infection either in the food reservoirs or any other sources.…”