Detection of enterotoxigenic K99 (F5) and F41 from fecal sample of calves by molecular and serological methods

Abstract

Enterotoxigenic Escherichia coli (ETEC) is one of the major causes of neonatal calf diarrhea. Almost all ETEC bacteria are known to adhere to receptors on the small intestinal epithelium via their fimbriae, (F5 (K99) and F41).This study was undertaken to investigate the phenotypic and genotypic screening of virulence genes in E. coli K99 and F41. During January 2008 to December 2009, 298 diarrheic neonatal calves at 1-30 days old were studied by multiplex PCR, isolation, and serological grouping. Of the 298 diarrheic samples, 268 E. coli were isolated, of which 16 samples (5.3%) were positive for having the F5 (K99) fimbrial gene by PCR while all of the E. coli isolates also carried F41 fimbrial genes. Twenty-five percent of the isolates were proven not to be toxigenic as they did not possess the STa enterotoxin gene.

Detection of enterotoxigenic Escherichia coli isolated from calves’ diarrhoea samples by molecular and serological methods

“…In the present study the prevalence of three virulence factors including K99 (F5), F41 and STa was similar to each other (11%) which was approximately in agreement with the result reported by Younis et al (10.36 %) [14]. Lower prevalence was reported by Shams et al [11], although higher result (14.1%) was found by Pourtaghi et al in previous investigation in another province of Iran [9], which indicates variety distribution of ETEC in different geographical areas. Despite to these differences in the prevalence of ETEC, all three virulence factor including K99, F41 and Sta were found in the both investigations.…”

“…Adhering of this pathogen to intestinal mucosa through its unique colonization factors as well as producing either heat-labile enterotoxins (LT-I and LT-II), heatstable enterotoxins (STa and STb), or both have been recognized as the main virulence factors of ETEC [3]. Several investigations reported the high prevalence of calf diarrhoea associated with ETEC around the world [11,14]. Considering to the importance of detection of ETEC in calves due to reducing mortality and economic loss in dairy farms, this study aimed to estimate the prevalence of ETEC in calves' diarrhoea samples by molecular and serological methods.…”

Clinical and sero-molecular characterization of <i>Escherichia coli</i> with an emphasis on hybrid strain in healthy and diarrheic neonatal calves in Egypt

“…8(4): 351-359 ________________________________________________________________________________________________________ 352 other E. coli strains have the ability to acquire virulence genes via HGT leading to the emergence of new pathotypes of E. coli (Müller et al, 2007) which pose a potential public health threat. Serological and molecular techniques are essential for detection and characterization of pathogenic E. coli and are based on O-H antigens and virulence markers, respectively (Nataro and Kaper, 1998;Ghanbarpour and Oswald, 2009;Bandyopadhyay et al, 2011;Nguyen et al, 2011;Shams et al, 2012). The clinical and hemato-biochemical alterations in E. coli diarrhea are complex in nature comprising serious imbalances of fluid, electrolyte and acid base status threatening the life of the calf (Singh et al, 2014).…”

Antimicrobial Resistance of Entrotoxigenic and Non-Entrotoxigenic Escherichia coli Isolated From Diarrheic Calves in Iran

“…According to previous studies, Escherichia coli (E. coli) is regarded as a major agent in the etiology of neonatal calf diarrhea; among diarrhoeagenic strains of E. coli, entrotoxigenic Escherichia coli (ETEC) is most commonly associated with diarrhea in calves and lambs (5,6). Several investigations reported the high prevalence of calf diarrhea caused by ETEC around the world (7)(8)(9).…”