Detection of Entamoeba histolytica in experimentally induced amoebic liver abscess: comparison of three staining methods

Ning, Tan Zi; Kin, Wong Weng; Mustafa, Shaymoli; Ahmed, Arefuddin; Noordin, Rahmah; Cheong, Tan Gim; Olivos-García, Alfonso; Lim, Boon Huat

doi:10.1016/s2221-1691(11)60191-3

Cited by 14 publications

(8 citation statements)

References 21 publications

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“…In some areas the overall prevalence of amoebic infection is as high as 50 percent. 11 Amoebiasis is prevalent throughout India and according to Mukharjii et al, prevalence rate is 3.6% in India. 12 Jain et al and Singla et al have found that intestinal perforation by amoebic colitis remains a common and an important cause for emergency colectomy.…”

Section: Discussionmentioning

confidence: 99%

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Comparison of efficacy of various stains and immunohistochemistry in diagnosis of amoebic colitis

Dhakar¹

2018

jmscr

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Section: Discussionmentioning

confidence: 99%

“…Literature search revealed that although IHC has been used for recognization of amoebic trophozoites but most of them are experimental studies. 11 No study has been found in literature in which IHC has been used for diagnosing amoebic colitis in human tissue. …”

mentioning

confidence: 99%

Comparison of efficacy of various stains and immunohistochemistry in diagnosis of amoebic colitis

Dhakar¹

2018

jmscr

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“…Histological analysis of tumor tissues and normal tissues was confirmed by our hospital using haematoxylin and eosin (H&E) stain as reported. 12 The study was carried out in accordance with the World Medical Association Declaration of Helsinki.…”

Section: Methodsmentioning

confidence: 99%

Anti-Tumor Effect of Celastrol on Hepatocellular Carcinoma by the circ_SLIT3/miR-223-3p/CXCR4 Axis

Si¹,

Wang²,

Xiao³

et al. 2021

CMAR

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Background Celastrol is a potential anti-tumor agent in hepatocellular carcinoma (HCC). Identifying the molecular determinants of the anti-HCC effect of celastrol is still challenging. In this study, we undertook to associate circular RNAs (circRNAs) with the anti-HCC molecular determinants of celastrol. Methods Cell colony formation, proliferation, migration, invasion and apoptosis were determined using the colony formation, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-2H-tetrazolium bromide (MTS), transwell and flow cytometry assays, respectively. The levels of circRNA slit guidance ligand 3 (circ_SLIT3), miR-223-3p and C-X-C motif chemokine receptor 4 (CXCR4) were gauged by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot. Ribonuclease R (RNase R) and actinomycin D assays were performed to assess the stability of circ_SLIT3. Targeted relationships among circ_SLIT3, miR-223-3p and CXCR4 were confirmed by the dual-luciferase reporter assay. In vivo assays were performed to detect the roles of celastrol and circ_SLIT3 on tumor growth in vivo. Results Celastrol repressed HCC cell proliferation, migration, invasion, and enhanced apoptosis in vitro and suppressed tumor growth in vivo. Celastrol down-regulated circ_SLIT3 expression in HCC cells, and celastrol exerted an anti-tumor effect on HCC in vitro and in vivo by down-regulating circ_SLIT3. Mechanistically, circ_SLIT3 directly interacted with miR-223-3p, and circ_SLIT3 controlled CXCR4 expression by sponging miR-223-3p. Moreover, miR-223-3p was involved in the celastrol/circ_SLIT3-mediated regulation on HCC progression. Furthermore, celastrol exerted the anti-HCC effect in vitro through the miR-223-3p/CXCR4 axis. Conclusion Our present work first identified the circ_SLIT3/miR-223-3p/CXCR4 axis as a novel mechanism of the anti-HCC effect of celastrol, providing a new insight into the involvement of circRNAs in the anti-tumor molecular determinants of celastrol.

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“…The parasites exhibited large, round eosinophilic nuclei and abundant vacuolated cytoplasm that changed from pink to magenta with periodic acid-Schiff (PAS) special stain, a technique to enhance detection of E. histolytica trophozoites; (Fig. 1) ( Ning et al , 2012 ). The morphology of these parasites was consistent with E. histolytica trophozoites.…”

Section: Treatmentmentioning

confidence: 99%

Amoeboma in a Saudi resident: a case report

et al. 2016

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Introduction: Amoebiasis is the third most frequent cause of mortality after malaria and schistosomiasis. In developed countries, amebiasis is also seen in migrants who have travelled to endemic areas. The factors responsible for its progression from intestinal amebiasis to an amebic liver abscess are not fully understood.Case presentation: A 54-year-old man presented with abdominal pain, fever and diarrhoea. Laparotomy confirmed an inflammatory mass involving the right colon, and he underwent a right hemicolectomy. He later developed abdominal distenstion due to an amoebic liver abscess and died from secondary nosocomial bacterial infection and surgical complications.Conclusion: Amoeboma is an uncommon manifestation of amoebiasis, and can mimic both carcinoma and inflammatory bowel disease; so, distinguishing between these two conditions is the key to providing appropriate therapy. Hepatic amoebiasis is the most common extraintestinal disease of invasive amoebiasis. This clinical report presents a case of an uncommon parasitic disease in Saudi Arabia and discusses the difficulties encountered while attempting to establish the correct diagnosis. Hence, a high index of suspicion is crucial for diagnosing Entamoeba histolytica to avoid unnecessary surgery and further complications.

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Detection of Entamoeba histolytica in experimentally induced amoebic liver abscess: comparison of three staining methods

Cited by 14 publications

References 21 publications

Comparison of efficacy of various stains and immunohistochemistry in diagnosis of amoebic colitis

Comparison of efficacy of various stains and immunohistochemistry in diagnosis of amoebic colitis

Anti-Tumor Effect of Celastrol on Hepatocellular Carcinoma by the circ_SLIT3/miR-223-3p/CXCR4 Axis

Amoeboma in a Saudi resident: a case report

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