Detection of electronegative low density lipoprotein (LDL−) in plasma and atherosclerotic lesions by monoclonal antibody-based immunoassays

Impedimetric immunosensor for electronegative low density lipoprotein (LDL−) based on monoclonal antibody adsorbed on (polyvinyl formal)–gold nanoparticles matrix

“…Therefore, LDL − was successfully detected in blood serum of normolipidemic subjects. Similar results were obtained by Damasceno et al [13] detecting LDL − from human blood plasma using ELISA and immunohistochemistry. These results are a strong indication that this system can be applicable to the construction of a biosensor for LDL − levels of human blood serum.…”

“…In general, the reactivity of many antibodies produced against oxidized LDL decreases with cleavage of hydrophobic domains, and by fragmentation and aggregation of Apo-B. This MAb seems to recognize epitopes in the minimally oxidized LDL [13]. The performance of the PVF-AuNPs-MAb-BSA system is shown in Table 2, for a fixed LDL − concentration, thereby confirming once again the ability of MAb to recognize LDL − lipoprotein after adsorption.…”

“…LDL − has a higher ␤-strand and unfolds with a lower ␣-helical structure, which may occur irrespective of oxidation when compared to native LDL [27]. The interaction between MAb-LDL − is due to the recognition of epitopes in a modified Apo-B [13]. The apolipoprotein B-100 (apo-B100) modifications observed in LDL − are related to a reduction in free amino groups due to their reaction with aldehydes formed by lipoperoxidation [28].…”

“…Although, Abdalla et al [12] by immunizing mice with LDL − isolated from human plasma using a HPLC, achieved 3D1036 MAb which binds to LDL − and does not bind to native LDL. This MAb can be used for detecting LDL − in rabbit, human plasma and atherosclerotic lesions [13]. A specific, simple, sensitive, and highly selective method for LDL − detection is therefore highly desirable.…”

Auto-anticorpos anti-LDLox e sua correlação com o perfil lipídico e o estado nutricional de adolescentes

“…7 According to Miller et al, 7 the products generated (malondialdehyde, conjugated dienes, hydroperoxides) have an elevated cytotoxic, chemotactic and proinflammatory potential. 8 The immunogenic capacity of oxLDL is also of note, since the particle and its autoantibodies have been identified in both atherosclerotic lesions 8,9 and in circulation. 8,10 Despite these observations, the role played by anti-oxLDL autoantibodies in the development of risk factors associated with MS in adolescents, particularly obese adolescents, has so far been little investigated.…”