Detection of EBV-encoded Small RNA from Diffuse Large B-cell Lymphoma Patients by RT-PCR Method

Abstract

Epstein-Barr virus (EBV) has a pathogenic role in several lymphomas including diffuse large B-cell lymphoma (DLBCL). In this study, we detected EBV from formalin-fixed paraffin embedded (FFPE) tissues of DLBCL patients by RT-PCR and compared the sensitivity of the RT-PCR method to in situ hybridization (ISH) method. The RNA was extracted from 91 FFPE samples with DLBCL and amplified with primers targeting EBV-encoded small RNA (EBER) by RT-PCR. When using the RT-PCR method, 13 of 91 patients (14.3%) were positive and among these 13 cases, 7 cases (7.7%) were from > 50-year-old patients that is classified as EBV positive DLBCL of the elderly. In previous results using ISH method, 3 of 91 patients (3.3%) were positive and 2 case (2.4%) were older than 50-year-old. These results indicate that RT-PCR method used in this study shows a higher sensitivity than ISH method. The ratio of male versus female among the EBV positive samples was 1.2:1 with the ratio of male higher. If RT-PCR method having high sensitivity is used simultaneously as well as the ISH method providing the information of the EBV positive cellular location, it is expected that EBV will be more accurately detected.

Triglyceride Regulates the Expression of M1 and M2 Macrophage-specific Markers in THP-1 Monocytes

“…cDNA was synthesized for 10 min at 25℃, 50 min at 37℃, and 15 min at 70℃. RT-PCR was performed as previously described (Yoo et al, 2015). cDNA amplification using 0.2 U of the Taq Premix PCR Kit (Genet Bio, Chungnam, Korea) was performed in a thermocycler using specific primers purchased from Genotech (Daejeon, Korea).…”