Detection of Dysplasia in Barrett's Esophagus With In Vivo Depth-Resolved Nuclear Morphology Measurements

Terry, Neil G.; Zhu, Yizheng; Rinehart, Matthew T.; Brown, William J.; Gebhart, Steven C.; Bright, Stephanie D.; Carretta, Elizabeth E.; Ziefle, Courtney; Panjehpour, Masoud; Galanko, Joseph A.; Madanick, Ryan D.; Dellon, Evan S.; Trembath, Dimitri G.; Bennett, Ana E.; Goldblum, John R.; Overholt, Bergein F.; Woosley, John T.; Shaheen, Nicholas J.; Wax, Adam

doi:10.1053/j.gastro.2010.09.008

Cited by 78 publications

(99 citation statements)

References 41 publications

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“…The combination of gating and difference in spectral behavior allows the epithelial nuclear scattering spectrum to be isolated in the processed light scattering spectroscopy (LSS) signal. A significant contribution from nuclear backscattering and clear correlation of dysplasia with nuclear size has been demonstrated in earlier studies 12–16,22–24 . Direct comparison of the nuclear size distribution extracted from the backscattering signal to that of histological examination of the corresponding H&E stained sections was also demonstrated 17,18 .…”

supporting

confidence: 63%

Light scattering spectroscopy identifies the malignant potential of pancreatic cysts during endoscopy

et al. 2017

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Pancreatic cancers are usually detected at an advanced stage and have poor prognosis. About one fifth of these arise from pancreatic cystic lesions. Yet not all lesions are precancerous, and imaging tools lack adequate accuracy for distinguishing precancerous from benign cysts. Therefore, decisions on surgical resection usually rely on endoscopic ultrasound-guided fine needle aspiration (EUS-FNA). Unfortunately, cyst fluid often contains few cells, and fluid chemical analysis lacks accuracy, resulting in dire consequences, including unnecessary pancreatic surgery for benign cysts and the development of cancer. Here, we report an optical spectroscopic technique, based on a spatial gating fibre-optic probe, that predicts the malignant potential of pancreatic cystic lesions during routine diagnostic EUS-FNA procedures. In a double-blind prospective study in 25 patients, with 14 cysts measured in vivo and 13 postoperatively, the technique achieved an overall accuracy of 95%, with a 95%confidence interval of 78–99%, in cysts with definitive diagnosis.

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supporting

confidence: 63%

Light scattering spectroscopy identifies the malignant potential of pancreatic cysts during endoscopy

et al. 2017

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“…As shown previously, these correlation lengths are roughly consistent with the cell nucleus size and cell to cell spacing [7]. However, simple Fourier based analysis using the correlation function analysis provides limited resolution (1.7 μm and 2.7 μm in θ 1 , and θ 2 directions in theory, but 6 μm and 3 μm measured) which may not be sufficient to discriminate the subtle nuclear size changes that reveal early cancer [3]. The size of the scatterers at the ROI was determined to be 9.7 μm in diameter, with index of the refraction of nuclei to be 1.43 and that of surrounding to be 1.37.…”

Section: Rat Esophagusmentioning

confidence: 73%

“…All the animal procedures for both the animal models were approved by the Duke Institutional Animal Care & Use Committee (IACUC). In the rat model, an esophagogastroduodenal anastomosis (EGDA) is performed to create chronic reflux of bile salts and stomach acid, mimicking reflux driven Barrett's Esophagus in humans [3]. Sprague Dawley rats were purchased from Charles River Laboratories (Wilmington, MA) at 5-6 weeks of age.…”

Section: Sample Preparationmentioning

confidence: 99%

“…The nuclear morphology data, specifically enlargement of the nuclei in the basal layer of the epithelium, have been shown to be a highly useful biomarker for the early detection of dysplasia, a precancerous tissue state. Previous studies have utilized a/LCI for evaluating epithelial dysplasia, including esophageal [3], colon [4], and cervical cancer [5].…”

Section: Introductionmentioning

confidence: 99%

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Analyzing spatial correlations in tissue using angle-resolved low coherence interferometry measurements guided by co-located optical coherence tomography

Kim

Heflin

Kresty

et al. 2016

Biomed. Opt. Express

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Angle-resolved low coherence interferometry (a/LCI) is an optical technique used to measure nuclear morphology in situ. However, a/LCI is not an imaging modality and can produce ambiguous results when the measurements are not properly oriented to the tissue architecture. Here we present a 2D a/LCI system which incorporates optical coherence tomography imaging to guide the measurements. System design and characterization are presented, along with example cases which demonstrate the utility of the combined measurements. In addition, future development and applications of this dual modality approach are discussed. and T. E. Milner, "Dual-modality fiber-based OCT-TPL imaging system for simultaneous microstructural and molecular analysis of atherosclerotic plaques," Biomed. Opt. Express 6(5), 1665-1678 (2015). 12. T. Liu, Q. Wei, J. Wang, S. Jiao, and H. F. Zhang, "Combined photoacoustic microscopy and optical coherence tomography can measure metabolic rate of oxygen," Biomed. Opt. Express 2(5), 1359-1365 (2011). 13. A. L. Clark, A. Gillenwater, R. Alizadeh-Naderi, A. K. El-Naggar, and R. Richards-Kortum, "Detection and diagnosis of oral neoplasia with an optical coherence microscope," J. Biomed. Opt. 9(6), 1271-1280 (2004). 14. T. S. Ralston, D. L. Marks, P. S. Carney, and S. A. Boppart, "Interferometric synthetic aperture microscopy," Nat. Phys. 3(2 Sidman, "Morphological, physiological, and biochemical changes in rhodopsin knockout mice," Proc. Natl. Acad. Sci. U.S.A. 96(2), 736-741 (1999). 20. J. W. Pyhtila, H. Ma, A. J. Simnick, A. Chilkoti, and A. Wax, "Analysis of long range correlations due to coherent light scattering from in-vitro cell arrays using angle-resolved low coherence interferometry," J. Biomed. Opt. 11(3), 034022 (2006). 21. A. Wax and J. W. Pyhtila, "In situ nuclear morphology measurements using light scattering as biomarkers of neoplastic change in animal models of carcinogenesis," Dis. Markers 25(6), 291-301 (2008).

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“…Due to these benefits, PAE [1,2] and other minimally invasive imaging techniques based on PAT [6,17,18] have been intensely developed for applications in such clinical areas as the cardiovascular [18][19][20][21][22][23][24][25], gastrointestinal [2,26,27], and urogenital [28][29][30][31] systems. These techniques are expected to provide a useful complement to other endoscopic modalities, such as endoscopic ultrasound (EUS) [32,33], endoscopic optical coherence tomography (OCT) [34][35][36][37][38][39][40], confocal endoscopy [41,42], and endoscopic polarized scanning spectroscopy [43,44].…”

Section: Introductionmentioning

confidence: 99%

A 25-mm diameter probe for photoacoustic and ultrasonic endoscopy

Yang¹,

Chen²,

Favazza³

et al. 2012

Opt. Express

112

124

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Abstract:We have created a 2.5-mm outer diameter integrated photoacoustic and ultrasonic mini-probe which can be inserted into a standard video endoscope's instrument channel. A small-diameter focused ultrasonic transducer made of PMN-PT provides adequate signal sensitivity, and enables miniaturization of the probe. Additionally, this new endoscopic probe utilizes the same scanning mirror and micromotor-based built-in actuator described in our previous reports; however, the length of the rigid distal section of the new probe has been further reduced to ~35 mm. This paper describes the technical details of the mini-probe and presents experimental results that both quantify the imaging performance and demonstrate its in vivo imaging capability, which suggests that it could work as a mini-probe for certain clinical applications. dual-modality OCT/LIF imaging using a novel VEGF receptor-targeted NIR fluorescent probe in the AOMtreated mouse model," Mol.

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Detection of Dysplasia in Barrett's Esophagus With In Vivo Depth-Resolved Nuclear Morphology Measurements

Cited by 78 publications

References 41 publications

Light scattering spectroscopy identifies the malignant potential of pancreatic cysts during endoscopy

Light scattering spectroscopy identifies the malignant potential of pancreatic cysts during endoscopy

Analyzing spatial correlations in tissue using angle-resolved low coherence interferometry measurements guided by co-located optical coherence tomography

A 25-mm diameter probe for photoacoustic and ultrasonic endoscopy

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