Detection of drug transporter expression using a 25-multiplex RT-PCR assay

Lee, Tae-Bum; Choi, Cheol-Hee

doi:10.1007/s10529-009-0039-9

Cited by 3 publications

(3 citation statements)

References 20 publications

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“…Based on the results of a study in an established 5-FU resistant cell line derived from SNUC5, SNUC5/5-FUR, 25-multiplex RT-PCR assay revealed that mRNA expression of four ATP-binding cassette transporter genes is up-regulated, whereas expression of a solute carrier transporter gene is down-regulated in SNUC5/5-FUR (Lee and Choi, 2009). Moreover, production of PGE 2 by COX-2 is up-regulated in SNUC5/5-FUR, suggesting that a COX-2 inhibitor might exert an anti-angiogenic effect in colon cancer (Choi et al ., 2011).…”

Section: Introductionmentioning

confidence: 99%

Reduced Autophagy in 5-Fluorouracil Resistant Colon Cancer Cells

Yao

Kang

Piao

et al. 2017

Biomolecules & Therapeutics

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We investigated the role of autophagy in SNUC5/5-FUR, 5-fluorouracil (5-FU) resistant SNUC5 colon cancer cells. SNUC5/5-FUR cells exhibited low level of autophagy, as determined by light microscopy, confocal microscopy, and flow cytometry following acridine orange staining, and the decreased level of GFP-LC3 puncta. In addition, expression of critical autophagic proteins such as Atg5, Beclin-1 and LC3-II and autophagic flux was diminished in SNUC5/5-FUR cells. Whereas production of reactive oxygen species (ROS) was significantly elevated in SNUC5/5-FUR cells, treatment with the ROS inhibitor N-acetyl cysteine further reduced the level of autophagy. Taken together, these results indicate that decreased autophagy is linked to 5-FU resistance in SNUC5 colon cancer cells.

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Section: Introductionmentioning

confidence: 99%

Reduced Autophagy in 5-Fluorouracil Resistant Colon Cancer Cells

Yao

Kang

Piao

et al. 2017

Biomolecules & Therapeutics

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“…After treatment, cells were washed once with PBS, immediately scraped and centrifuged at 13,000 rpm for 1 min. Cell pellets were frozen in liquid nitrogen for 5 min and stored at -80℃ until analyzed as previously described (22). In brief, isolated RNA was reverse transcribed using reverse transcriptase (Invitrogen) and an oligo(dT) primer at 37℃ for 1 hr.…”

Section: Methodsmentioning

confidence: 99%

2,3,7,8-Tetrachlorodibenzo-P-Dioxin Induced Cell-Specific Drug Transporters With Acquired Cisplatin Resistance in Cisplatin Sensitive Cancer Cells

et al. 2014

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2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) can induce drug transporter genes such as the ATP-binding cassette G member 2 (ABCG2), which contributes to multidrug resistance. We investigated the effect of TCDD pretreatment on drug transporters induction from cancer cells of various origins. Cell viabilities after treatment of cisplatin were measured to evaluate acquiring cisplatin resistance by TCDD. Acquring cisplatin resistance was found only in cisplatin senstivie cancer cells including gastric SNU601, colon LS180, brain CRT-MG and lymphoma Jurkat cells which showed a significant increase in cell viability after combined treatment with TCDD and cisplatin. High increase of ABCG2 gene expression was found in SNU601 and LS180 cells with a mild increase in the expression of the ABCC3, ABCC5,and SLC29A2 genes in SNU601 cells, and of major vault protein (MVP) in LS180 cells. The AhR inhibitor kaempferol suppressed the upregulation of ABCG2 expression and reversed the TCDD-induced increase in cell viability in LS180 cells. However, in CRT-MG cells, other transporter genes including ABCC1, ABCC5, ABCA3, ABCA2, ABCB4, ABCG1, and SLC29A1 were up-regulated. These findings suggested the acquiring cisplatin resistance by TCDD associated with cancer cell-type-specific induction of drug transporters.Graphical Abstract

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“…ABCB5 protein is expressed in cells refractory to 5‐FU treatment . ABCG2 and ABCA3 were not confirmed to be responsible for the efflux of 5‐FU or its metabolites; however, the overexpression of these genes was demonstrated in the 5‐FU resistant colorectal carcinoma cell line SNU‐5‐FUR .…”

Section: Introductionmentioning

confidence: 95%