Detection of DNA within fingermarks

Haines, Alicia M; Tobe, Shanan S.; Kobus, Hilton; Linacre, Adrian

doi:10.1016/j.fsigss.2013.10.148

Cited by 13 publications

(7 citation statements)

References 7 publications

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“…In these latter instances, it may be beneficial to apply visualisation techniques, e.g. gold or silver vacuum metal deposition, fluorescent in-situ detection [231][232][233][234], to try to locate the areas of contact for increased sampling accuracy.…”

Section: Targetingmentioning

confidence: 99%

DNA transfer in forensic science: A review

Oorschot

Szkuta

Meakin³

et al. 2019

Forensic Science International: Genetics

214

130

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Understanding the variables impacting DNA transfer, persistence, prevalence and recovery (DNA-TPPR) has become increasingly relevant in investigations of criminal activities to provide opinion on how the DNA of a person of interest became present within the sample collected. This review considers our current knowledge regarding DNA-TPPR to assist casework investigations of criminal activities. There is a growing amount of information available on DNA-TPPR to inform the relative probabilities of the evidence given alternative scenarios relating to the presence or absence of DNA from a specific person in a collected sample of interest. This information should be used where relevant. However, far more research is still required to better understand the variables impacting DNA-TPPR and to generate more accurate probability estimates of generating particular types of profiles in more casework relevant situations. This review explores means of achieving this. It also notes the need for all those interacting with an item of interest to have an awareness of DNA transfer possibilities post criminal activity, to limit the risk of contamination or loss of DNA. Appropriately trained forensic practitioners are best placed to provide opinion and guidance on the interpretation of profiles at the activity level. However, those requested to provide expert opinion on DNA-related activity level issues are often insufficiently trained to do so. We advocate recognition of DNA activity associated expertise to be distinct from expertise associated with the identification of individuals. This is to be supported by dedicated training, competency testing, authorisation, and regular fit for purpose proficiency testing. The possibilities for experts to report on activity-related issues will increase as our knowledge increases through further research, access to relevant data is enhanced, and tools to assist interpretations are better exploited. Improvement opportunities will be achieved sooner, if more laboratories and agencies accept the need to invest in these aspects as well as the training of practitioners.

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Section: Targetingmentioning

confidence: 99%

DNA transfer in forensic science: A review

Oorschot

Szkuta

Meakin³

et al. 2019

Forensic Science International: Genetics

214

130

View full text Add to dashboard Cite

show abstract

“…The use of fluorescent nucleic acid staining dyes (e.g., Diamond™ dye, SYBR® Green I, and GelGreen™) has been developed recently for detecting latent DNA on items [5,[11][12][13][14][15][16]. A recent study reported monitoring the deposited cell number within entire thumbprints on a glass slide through a "single touch" event using DD staining [6]; however, a comparison of the visualized cells number from the entire and a part of the thumbprint was not part of this report [6].…”

Section: Highlightsmentioning

confidence: 99%

Monitoring cell loss through repetitive deposition

Petcharoen

Kirkbride

Linacre

2022

Journal of Forensic Sciences

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Touch DNA deposited on items can be visualised by using fluorescent nucleic acid staining dyes. It might be expected that if a person contacts items multiple times, then at each contact fewer cells should be transferred and deposited. Here we report on the use of Diamond Dye (DD) to monitor any reduction in cellular deposition during multiple contacts. A volunteer, who was assigned as a heavy shedder, was asked to deposit a thumbprint using both left and right thumbs for 15 s onto separate clean glass slides. Thumbprints were collected in triplicate with each mark made for 15 s. Immediately after deposition, a second and then third mark was made in the same way. The three consecutive depositions were repeated 30 times to create 90 tested thumbprints. The number of cells within each entire thumbprint was scored using a cell‐counting program, developed in‐house. The number of cells deposited by the second and third depositions showed a decrease in the cell number compared to the first deposition, by ~70% and ~85% respectively. There was no difference between the percentage of cell persistence from whole thumbprints (16 frames) compared to only scoring part of thumbprints (4 frames). The data obtained provide insight into how cells are deposited by touch and how many cells remain on the thumb for subsequent contact events. Such information may account for cell deposition when performing actions (e.g. loading a firearm or repetitively opening a closing zip‐lock bag).

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“…diamond dye, DNA staining, fluorescence, latent DNA, trace DNA, forensic biology by either a swab or tape-lift, is based on best assumptions and it is not known if DNA is present until the quantification step in the laboratory [5][6][7]. For many such samples, a partial or no DNA profile will be obtained from this process; for example, 38% of serious crime and 17% of high-volume crime samples in the Netherlands produced no DNA profiles [8].…”

Section: Introductionmentioning

confidence: 99%

“…Visualizing the location of cellular material, and therefore the source of DNA, will allow examiners to target sampling to areas where DNA is present, and not sample areas where DNA is absent. Without knowledge of cellular material location, collection of DNA, by either a swab or tape‐lift, is based on best assumptions and it is not known if DNA is present until the quantification step in the laboratory [5–7]. For many such samples, a partial or no DNA profile will be obtained from this process; for example, 38% of serious crime and 17% of high‐volume crime samples in the Netherlands produced no DNA profiles [8].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of a fluorescent dye to visualize touch DNA on various substrates

Champion

Kanokwongnuwut

Oorschot

et al. 2021

Journal of Forensic Sciences

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The ability to visualize the presence of cellular material deposited by touch onto items of forensic importance has the potential to greatly aid in criminal investigations. It is now routine in forensic casework to examine items for DNA assumed to have been deposited by touch [1-3]. Such DNA is often referred to as 'touch DNA' even though samples collected from such deposits can also include DNA from undetermined biological sources other than that derived from skin and/or background DNA from actions other than touching [4]. Visualizing the location of cellular material, and therefore the source of DNA, will allow examiners to target sampling to areas where DNA is present, and not sample areas where DNA is absent.Without knowledge of cellular material location, collection of DNA,

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Detection of DNA within fingermarks

Cited by 13 publications

References 7 publications

DNA transfer in forensic science: A review

DNA transfer in forensic science: A review

Monitoring cell loss through repetitive deposition

Evaluation of a fluorescent dye to visualize touch DNA on various substrates

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