Detection of DNA utilizing a fluorescent reversible change of a biosensor based on the electron transfer from quantum dots to polymyxin B sulfate

Wang, Linlin; Liu, Shaopu; Liang, Wanjun; Li, Dan; Yang, Jinlong; He, Yonghong

doi:10.1016/j.jcis.2015.02.034

Cited by 14 publications

(7 citation statements)

References 41 publications

(38 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Together, our data and others demonstrate polymyxin B affinity for DNA (Fig. 5A) (Kong et al 2011; Wang et al 2015). A previous study using fluorescent polymyxin probes showed that polymyxin primarily localizes in the ER and mitochondria in cultured rat renal tubular cells, with only modest signals in the nucleus (Yun et al 2015) This discrepancy is likely due to the recognition epitope of the antibody, reported to specifically recognise the conformational hydrophobic entities (fatty acyl tail and position 6/7 hydrophobic residues) and cyclic backbone (Velkov et al 2016).…”

Section: Discussionsupporting

confidence: 88%

“…As polymyxin B interacts with DNA ((Kong et al 2011; Wang et al 2015) and later section), we hypothesized that polymyxin B treatment led to genome instability. We first measured the appearance of micronuclei, a hallmark of genome instability (Fenech 2002).…”

Section: Resultsmentioning

confidence: 99%

“…Multiple studies have suggested that polymyxin B binds to DNA (Kong et al 2011; Wang et al 2015). Given its amphipathic structure, it is thought that polymyxin B binds DNA non-covalently via electrostatic attraction, groove-binding, intercalation or a combination of these (Kong et al 2011; Wang et al 2015). Kong et al previously utilised polymyxin B as a probe to determine the DNA concentrations of calf thymus, salmon and herring sperm (Kong et al 2011).…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Polymyxin B causes DNA damage in HK-2 cells and mice

et al. 2018

View full text Add to dashboard Cite

Increasing incidence of multidrug-resistant bacteria presents an imminent risk to global health. Polymyxins are 'last-resort' antibiotics against Gram-negative 'superbugs'; however, nephrotoxicity remains a key impediment in their clinical use. Molecular mechanisms underlying this nephrotoxicity remain poorly defined. Here, we examined the pathways which led to polymyxin B induced cell death in vitro and in vivo. Human proximal tubular cells were treated with polymyxin B (12.5-100 μM) for up to 24 h and showed a significant increase in micronuclei frequency, as well as abnormal mitotic events (over 40% in treated cells, p < 0.05). Time-course studies were performed using a mouse nephrotoxicity model (cumulative 72 mg/kg). Kidneys were collected over 48 h and investigated for histopathology and DNA damage. Notable increases in γH2AX foci (indicative of double-stranded breaks) were observed in both cell culture (up to ~ 44% cells with 5+ foci at 24 h, p < 0.05) and mice treated with polymyxin B (up to ~ 25%, p < 0.05). Consistent with these results, in vitro assays showed high binding affinity of polymyxin B to DNA. Together, our results indicate that polymyxin B nephrotoxicity is associated with DNA damage, leading to chromosome missegregation and genome instability. This novel mechanistic information may lead to new strategies to overcome the nephrotoxicity of this important last-line class of antibiotics.

show abstract

Section: Discussionsupporting

confidence: 88%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Polymyxin B causes DNA damage in HK-2 cells and mice

et al. 2018

View full text Add to dashboard Cite

show abstract

“…For example, Li’s group discriminated a parallel-stranded G-quadruplex from DNA with other topologies and structures by the RRS method [ 29 ]. In addition, the RRS technique has been widely applied to the determination of surfactants [ 30 ], metal ions [ 31 ], proteins [ 32 ], etc.…”

Section: Introductionmentioning

confidence: 99%

A resonance Rayleigh scattering sensor for sensitive differentiation of telomere DNA length and monitoring special motifs (G-quadruplex and i-motif) based on the Ag nanoclusters and NAND logic gate responding to chemical input signals

et al. 2018

View full text Add to dashboard Cite

BackgroundDifferentiation of telomere length is of vital importance because telomere length is closely related with several deadly diseases such as cancer. Additionally, G-quadruplex and i-motif formation in telomeric DNA have been shown to act as a negative regulator of telomere elongation by telomerase in vivo and are considered as an attractive drug target for cancer chemotherapy.ResultsIn this assay, Ag nanoclusters templated by hyperbranched polyethyleneimine (PEI–Ag NCs) are designed as a new novel resonance Rayleigh scattering (RRS) probe for sensitive differentiation of telomere length and monitoring special motifs (G-quadruplex and i-motif). In this assay, free PEI–Ag NC probe or DNA sequence alone emits low intensities of RRS, while the formation of PEI–Ag NCs/DNA complexes yields greatly enhanced RRS signals; however, when PEI–Ag NCs react with G-quadruplex or i-motif, the intensities of RRS exhibit slight changes. At the same concentration, the enhancement of RRS signal is directly proportional to the length of telomere, and the sensitivity of 64 bases is the highest with the linear range of 0.3–50 nM (limit of detection 0.12 nM). On the other hand, due to the conversion of telomere DNA molecules among multiple surrounding conditions, a DNA logic gate is developed on the basis of two chemical input signals (K+ and H+) and a change in RRS intensity as the output signal.ConclusionOur results indicate that PEI–Ag NCs can serve as a novel RRS probe to identify DNA length and monitor G-quadruplex/i-motif through the different increasing degrees of RRS intensity. Meanwhile, the novel attributes of the nanoprobe stand superior to those involving dyes or labeled DNA because of no chemical modification, low cost, green, and high efficiency.Electronic supplementary materialThe online version of this article (10.1186/s12951-018-0407-5) contains supplementary material, which is available to authorized users.

show abstract

“…Among these, fluorescence, which can offer prominent merits in terms of sensitivity and easy operation, has attracted much attention. For example, He’s group has employed QDs to design several fluorescence probes, providing high sensitivity and specificity to detect DNA in aqueous solution. − Despite this progress, the application for the direct imaging of endogenous DNA in live cells is still challenging, presumably due to the low cell uptake, which cannot produce sensitive fluorescence signals to tell the DNA concentration. As such, the development of new DNA activatable fluorescence probes capable of reporting on DNA levels in live cells is highly demanded.…”

Section: Introductionmentioning

confidence: 99%

Activatable QD-Based Near-Infrared Fluorescence Probe for Sensitive Detection and Imaging of DNA

Shen

Zhang

Sun

et al. 2017

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

Accurate detection of DNA is essential for the precise diagnosis of diseases. Here we report an activatable near-infrared (NIR) fluorescence nanoprobe (QD-Al-GFLX) composed of NIR quantum dots (QDs) and Al(III)-gatifloxacin (Al-GFLX) complexes for the sensitive detection of double-stranded DNA (dsDNA) both in aqueous solution and in living cells. We demonstrated that the initial strong NIR fluorescence of QDs in QD-Al-GFLX was quenched by the Al-GFLX complex via a photoinduced electron transfer (PET) mechanism. Upon interaction with dsDNA, the high binding affinity between dsDNA and Al-GFLX complex could trigger QD-Al-GFLX dissociation, which could eliminate the PET process, resulting in significant enhancement of NIR fluorescence. QD-Al-GFLX was sensitive and specific to detect dsDNA in aqueous solution, with a detection limit of 6.83 ng/mL. The subsequent fluorescence imaging revealed that QD-Al-GFLX holds a high ability to enter into live cells, generating strong NIR fluorescence capable of reporting on dsDNA levels. This study highlighted the potential of using QD-Al-GFLX nanoprobe for the real-time detection and imaging of dsDNA in living cells.

show abstract

Detection of DNA utilizing a fluorescent reversible change of a biosensor based on the electron transfer from quantum dots to polymyxin B sulfate

Cited by 14 publications

References 41 publications

Polymyxin B causes DNA damage in HK-2 cells and mice

Polymyxin B causes DNA damage in HK-2 cells and mice

A resonance Rayleigh scattering sensor for sensitive differentiation of telomere DNA length and monitoring special motifs (G-quadruplex and i-motif) based on the Ag nanoclusters and NAND logic gate responding to chemical input signals

Activatable QD-Based Near-Infrared Fluorescence Probe for Sensitive Detection and Imaging of DNA

Contact Info

Product

Resources

About