Detection of DNA methylation in DBF1 gene of maize inbred W64A and mutant vp14 exposed to drought stress

Sallam, Nehal; Moussa, Mohamed; Yacout, Mohamed; Shakam, H.

doi:10.1007/s42976-021-00160-2

Cited by 3 publications

(2 citation statements)

References 45 publications

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“…The average relative water content were highly significant [128] Stop watering for 15d when growth reaches the 5 Plant dry weight and leaf area were significantly reduced, significantly [137] applied with 20% PEG 6000 for 72 h at the six-leaf stage inhibits ΦPSII and increased ΦNPQ, higher photosynthetic rate and stomatal conductance Based on previous studies, we found that plants have DNA methylation level changes in response to drought stress, which is directly reflected in a more pronounced proportion of methylation changes compared with control material treated with regular moisture. In particular, most of the increase in DNA methylation levels occurred genome-wide, specifically, two studies in Table 1 showed a genome-wide decrease in DNA methylation levels, and seven studies showed an increase in DNA methylation levels; whereas the increase or decrease in methylation levels of the internal part of the promoter was specifically determined by the positive or negative regulation of the gene under drought stress; the proportion of changes mostly ranged from 10%-20%, but some showed only slight changes, which may be related to tissue specificity; In contrast, the proportions and trends of methylation sites in the three contexts were different, with the trends of symmetric methylation sites such as CG and CHG remaining the same, whereas most of the CHH asymmetric methylation sites were opposite to the symmetric methylation sites, which might be more sensitive to drought environment.…”

Section: Dbf1mentioning

confidence: 99%

Recent Advances in The Plant Genomic DNA Methylation Studies and Its Involvement in Regulating Drought Stress Response in Crops

Fan,

Sun,

Yan

et al. 2024

Preprint

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As the global arid climate intensifies and the groundwater becomes increasingly scarce, drought stress has become a major challenge to plant growth and development worldwide, especially causing yield reduction and extinction of cultivated crops. In a large number of drought resistance studies, DNA methylation has been found dynamically associated with response to drought stress in plants by regulating gene expression and developments in plants, but the specific mechanisms are not yet clear. In this article, the current advances of research have been summarized on DNA methylation response to drought stress in various plant species, in terms of methylation detection methods, mechanisms of pattern changes (DNA de novo methylation, DNA maintenance methylation, and DNA demethylation), and its response to drought stress. With Most of the studies showed significant changes in genome or gene promoter methylation levels in plants subjected to drought stress, but the identification of the genes and pathways involved is still in the minority. The aim is to provide references for in-depth research on the response of plants to drought stress through epigenetics, and to uncover drought resistance genes regulated by DNA methylation, specific signaling pathways, and molecular mechanisms of action.

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Section: Dbf1mentioning

confidence: 99%

Recent Advances in The Plant Genomic DNA Methylation Studies and Its Involvement in Regulating Drought Stress Response in Crops

Fan,

Sun,

Yan

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…Plant dry weight and leaf area were significantly reduced, ΦPSII and increased ΦNPQ, higher photosynthetic rate and stomatal conductance [143] Based on our review of previous studies, we found that in general, plants change the extent of DNA methylation in response to drought stress. While two studies in Table 1 showed a genome-wide decrease in DNA methylation levels [122,125], and six studies showed an genome-wide increase in DNA methylation levels [124,128,136,139,140,142], an increase or decrease in methylation levels of the internal part of promoters of drought response genes was specifically determined by the positive or negative regulation of the gene under drought stress [126,127,130,134,135,137,140,143]. The proportion of methylation changes in response to drought mostly ranged from 10 to 20%, but some showed only slight changes, which may be related to tissue specificity [144].…”

Section: Tap5cs Tabadhmentioning

confidence: 99%

Recent Advances in Studies of Genomic DNA Methylation and Its Involvement in Regulating Drought Stress Response in Crops

Fan,

Sun,

Yan

et al. 2024

Plants

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As global arid conditions worsen and groundwater resources diminish, drought stress has emerged as a critical impediment to plant growth and development globally, notably causing declines in crop yields and even the extinction of certain cultivated species. Numerous studies on drought resistance have demonstrated that DNA methylation dynamically interacts with plant responses to drought stress by modulating gene expression and developmental processes. However, the precise mechanisms underlying these interactions remain elusive. This article consolidates the latest research on the role of DNA methylation in plant responses to drought stress across various species, focusing on methods of methylation detection, mechanisms of methylation pattern alteration (including DNA de novo methylation, DNA maintenance methylation, and DNA demethylation), and overall responses to drought conditions. While many studies have observed significant shifts in genome-wide or gene promoter methylation levels in drought-stressed plants, the identification of specific genes and pathways involved remains limited. This review aims to furnish a reference for detailed research into plant responses to drought stress through epigenetic approaches, striving to identify drought resistance genes regulated by DNA methylation, specific signaling pathways, and their molecular mechanisms of action.

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Epigenetic Modifications of Hormonal Signaling Pathways in Plant Drought Response and Tolerance for Sustainable Food Security

Kaya,

Uğurlar,

Adamakis

2024

IJMS

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Drought significantly challenges global food security, necessitating a comprehensive understanding of plant molecular responses for effective mitigation strategies. Epigenetic modifications, such as DNA methylation and histone modifications, are key in regulating genes and hormones essential for drought response. While microRNAs (miRNAs) primarily regulate gene expression post-transcriptionally, they can also interact with epigenetic pathways as potential effectors that influence chromatin remodeling. Although the role of miRNAs in epigenetic memory is still being explored, understanding their contribution to drought response requires examining these indirect effects on epigenetic modifications. A key aspect of this exploration is epigenetic memory in drought-adapted plants, offering insights into the transgenerational inheritance of adaptive traits. Understanding the mechanisms that govern the maintenance and erasure of these epigenetic imprints provides nuanced insights into how plants balance stability and flexibility in their epigenomes. A major focus is on the dynamic interaction between hormonal pathways—such as those for abscisic acid (ABA), ethylene, jasmonates, and salicylic acid (SA)—and epigenetic mechanisms. This interplay is crucial for fine-tuning gene expression during drought stress, leading to physiological and morphological adaptations that enhance plant drought resilience. This review also highlights the transformative potential of advanced technologies, such as bisulfite sequencing and CRISPR-Cas9, in providing comprehensive insights into plant responses to water deficit conditions. These technologies pave the way for developing drought-tolerant crops, which is vital for sustainable agriculture.

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Detection of DNA methylation in DBF1 gene of maize inbred W64A and mutant vp14 exposed to drought stress

Cited by 3 publications

References 45 publications

Recent Advances in The Plant Genomic DNA Methylation Studies and Its Involvement in Regulating Drought Stress Response in Crops

Recent Advances in The Plant Genomic DNA Methylation Studies and Its Involvement in Regulating Drought Stress Response in Crops

Recent Advances in Studies of Genomic DNA Methylation and Its Involvement in Regulating Drought Stress Response in Crops

Epigenetic Modifications of Hormonal Signaling Pathways in Plant Drought Response and Tolerance for Sustainable Food Security

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