1998
DOI: 10.1016/s1383-5718(97)00192-7
|View full text |Cite
|
Sign up to set email alerts
|

Detection of DNA lesions induced by chemical mutagens by the single cell gel electrophoresis (Comet) assay

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

1
13
0
2

Year Published

2000
2000
2017
2017

Publication Types

Select...
9
1

Relationship

1
9

Authors

Journals

citations
Cited by 52 publications
(16 citation statements)
references
References 11 publications
1
13
0
2
Order By: Relevance
“…The dispersion of the tail moment, ie variance/mean, is also shown. Bleomycin (100 g/ml), used as a genotoxic agent able to produce DNA lesions just after treatment, 53 displayed an increase in DNA damage (mean tail moments were 27.23 ± 1.68 m in WBC and 30.27 ± 2.06 m in K562, respectively). Styrene (50 M), a compound which is metabolized by cytochrome P450 isozymes to reactive epoxides, 54 was used both with and without S9 to show the effectiveness of the S9 mix.…”
Section: Resultsmentioning
confidence: 99%
“…The dispersion of the tail moment, ie variance/mean, is also shown. Bleomycin (100 g/ml), used as a genotoxic agent able to produce DNA lesions just after treatment, 53 displayed an increase in DNA damage (mean tail moments were 27.23 ± 1.68 m in WBC and 30.27 ± 2.06 m in K562, respectively). Styrene (50 M), a compound which is metabolized by cytochrome P450 isozymes to reactive epoxides, 54 was used both with and without S9 to show the effectiveness of the S9 mix.…”
Section: Resultsmentioning
confidence: 99%
“…MMC causes DNA crosslinking lesions that inhibit DNA unwinding and fragmentation. However, Miyamae et al (1998) reported that DNA migration in mouse lymphoma cells significantly increases at 21 h after treatment. Therefore, such long time incubation might also yield a clearly detectable DNA damage.…”
Section: Discussionmentioning
confidence: 99%
“…The former is very important so as to avoid pseudonegative results and the comet assay can detecta wide variety of genotoxic compounds both in vitro [35,36] and in vivo [3,[37][38][39][40]. The micronucleus test (MN test) is a standard procedure that can detect structural chromosome aberrations derived from initial damage in the S phase and/or numerical chromosome aberrations due to an eugenic effects in the M phase [40,41].…”
Section: Power Of the Comet Assay To Detect Low Level Genotoxicitymentioning
confidence: 99%