Detection of DNA hybridization by ABEI electrochemiluminescence in DNA-chip compatible assembly

Calvo-Muñoz, Maria-Luisa; Dupont-Filliard, A.; Billon, M.; Guillerez, S.; Bidan, Gérard; Marquette, Christophe A.; Blum, Loı̈c J.

doi:10.1016/j.bioelechem.2004.04.009

Cited by 33 publications

(17 citation statements)

References 10 publications

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“…Luminol-H 2 O 2 -based method [148,149] and hot electron-induced ECL [150] have also been reported. In the former case, immobilization of N-(4-aminobutyl)-N-etylisoluminol (ABEI), a luminol derivative, on a target ssDNA was employed for the ECL detection of the hybridization between a complementary probe ssDNA covalently linked to a polypyrrole support and the target ssDNA [149].…”

Section: Dna Detection and Quantificationmentioning

confidence: 98%

“…In the former case, immobilization of N-(4-aminobutyl)-N-etylisoluminol (ABEI), a luminol derivative, on a target ssDNA was employed for the ECL detection of the hybridization between a complementary probe ssDNA covalently linked to a polypyrrole support and the target ssDNA [149]. In hot electron-induced ECL system, hybridization was detected using hot electroninduced ECL of the tetramethylrhodamine dye.…”

Section: Dna Detection and Quantificationmentioning

confidence: 99%

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Applications of Electrochemiluminescence

Parveen

Aslam

et al. 2013

SpringerBriefs in Molecular Science

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The development of electrochemiluminescence (ECL) applications is a growing field, having the potential advantages of ECL over conventional chemiluminescence. ECL has found various applications in immunoassays, DNA probe assays, and aptasensors by employing ECL-active species as labels on biological molecules. The recent use of ECL to detect many chemically, biochemically, clinically, and environmentally important analytes is reviewed.

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Section: Dna Detection and Quantificationmentioning

confidence: 98%

Section: Dna Detection and Quantificationmentioning

confidence: 99%

Applications of Electrochemiluminescence

Parveen

Aslam

et al. 2013

SpringerBriefs in Molecular Science

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“…25,26 Briefly, the PCT antibody was diluted to 3.2 mg/mL using 0.1 mol/L PBS (pH 7.4). Five hundred microliters containing 165 mg/ml ABEI, 35 mg/mL NHS, and 15 mg/mL EDC was added to 500 μL of the 3.2 mg/mL anti-PCT solution and incubated for 1 h at 37 C. The unreacted reagents were removed via dialysis for 2 days with 0.1 mol/L PBS (pH 7.4).…”

Section: The Abei-labeled Anti-pct Antibodymentioning

confidence: 99%

Determining the Concentration of Procalcitonin Using a Magnetic Particles-based Chemiluminescence Assay for the Clinical Diagnosis of Sepsis

Rao

et al. 2013

ANAL. SCI.

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Our objective is to develop an assay based on magnetic particles (MPs) to determine the concentration of procalcitonin (PCT) using a chemiluminescence immunoassay (CLIA). Fluorescein isothiocyanate (FITC) and N-(aminobutyl)-N-(ethylisoluminol) (ABEI) were used to label two different anti-procalcitonin (PCT) monoclonal antibodies. The labeled antibodies, the PCT antigen, and the anti-FITC antibody-coated MPs formed a double-sandwiched immunocomplex. The measured relative light units (RLUs) of ABEI in the substrate solution were directly proportional to the amount of PCT present in the samples. The proposed method was linear to 600 ng/mL with a detection limit of 0.03 ng/mL. The coefficient of variation (CV) was <5% and <6% for the intra-and inter-assay precision, respectively. The average recoveries were between 95 and 107%. The linearity-dilution effect gave a linear correlation coefficient of 0.9912. This proposed assay provided an alternative method to quantitatively measure PCT in serum for the diagnosis of sepsis.

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“…As a powerful analytical technique, ECL has become an important detection method in analytical chemistry in recent years [22][23][24][25][26][27]. ECL has the advantage of high sensitivity and good reproducibility and can be controlled easily.…”

Section: Introductionmentioning

confidence: 99%