Detection of DNA fragments separated by capillary electrophoresis based on their native fluorescence inside a sheath flow

McGregor, David A.; Yeung, Edward S.

doi:10.1016/0021-9673(94)85147-6

Cited by 33 publications

(20 citation statements)

References 20 publications

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“…Use of CE in DNA analysis can dramatically reduce separation times because high electric fields can be utilized without heat buildup in the separation media, which can reduce resolution and degrade the sample. Aqueous solutions of hydrophilic polymers such as methylcellulose [1][2][3][4], hydroxyethylcellulose (HEC) [5,6,14], hydroxypropylcellulose (HPC) [7,14], hydroxypropylmethylcellulose [7,8], linear polyacrylamide (PAA) [9][10][11]14], poly-N,N-dimethylacrylamide (pDMA) [15], polyvinyl alcohol [8,12], and polyethyleneoxide (PEO) [12,13] have been used successfully as separation media in CE. The relative importance of polymer properties such as persistence length, hydrophilicity, and molecular mass in altering electrophoretic mobility of polyelectrolyte analytes is still not well understood, however.…”

Section: General Aspectsmentioning

confidence: 99%

Effect of matrix chain length on the electrophoretic mobility of large linear and branched DNA in polymer solutions

Saha¹,

Heuer

Archer

2004

Electrophoresis

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We study the effect of matrix chain molecular weight Mw and concentration c on the electrophoretic mobility micro of large linear and star-like, branched DNA in polymer solutions. Polyethylene oxide (PEO) with narrow molecular weight distributions form the main focus of this study. For PEO concentrations ranging from one half the overlap concentration, c*, to 3c*, the effective drag coefficient, zeta is identical with (mu0/mu) - 1, satisfies the following approximate scaling relationship, zeta approximately cMw(0.7). Here, mu0 is the electrophoretic mobility in free solution. While the concentration dependence is consistent with predictions from the transient entanglement coupling (TEC) model, the molecular weight dependence is significantly weaker. Although a similar dependence of mobility on Mw can be predicted when nonentangling collisions are the dominant source of drag, a model based on these collisions alone cannot reproduce the experimental observations. We also find that the architecture of large DNA does not affect either the concentration dependence or molecular weight dependence of the electrophoretic mobility.

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Section: General Aspectsmentioning

confidence: 99%

Effect of matrix chain length on the electrophoretic mobility of large linear and branched DNA in polymer solutions

Saha¹,

Heuer

Archer

2004

Electrophoresis

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“…This is particularly true in capillary gel electrophoresis, where the sieving gel can produce a large scattering background. Indeed, formats using off-column detection such as the sheath flow cell [13,14] have been implemented to reduce this background problem.…”

Section: Introductionmentioning

confidence: 99%

Near-infrared laser-induced fluorescence detection in capillary electrophoresis

McWhorter

Soper

2000

Electrophoresis

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“…Using MC for DNA separations has been proposed by many researchers [112][113][114][115]. Good separation of small DNA fragments from 100 to 500 bp could be achieved in 4 min in 0.5% MC at 700 V/cm with an average plate efficiency up to 2.5610 6 /m [116].…”

Section: Methylcellulose (Mc)mentioning

confidence: 99%

Polymer solutions and entropic‐based systems for double‐stranded DNA capillary electrophoresis and microchip electrophoresis

Baba

2004

Electrophoresis

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We give an overview of recent development of low-viscosity polymer solutions and entropic trapping networks for double-stranded DNA (dsDNA) separations by conventional capillary electrophoresis and microchip electrophoresis. Theoretical models for describing separation mechanisms, commonly used noncross-linked polymer solutions, thermoresponsive (viscosity-adjustable) polymer solutions, and novel entropic trapping networks are included. The thermoresponsive polymer solutions can be loaded at one temperature into microchannels at lower viscosities, and used in separation at another temperature at entanglement threshold concentrations and higher viscosities. The entropic-based separations use only arrays of regular obstacles acting as size-separations and do not need viscous polymer solutions. These progresses have potential in integration to automated capillary and microfluidic chip systems, enabling better reusability of separation microchannels, much shorter DNA separation times, and higher reproducibility due to less matrix degradation.

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Detection of DNA fragments separated by capillary electrophoresis based on their native fluorescence inside a sheath flow

Cited by 33 publications

References 20 publications

Effect of matrix chain length on the electrophoretic mobility of large linear and branched DNA in polymer solutions

Effect of matrix chain length on the electrophoretic mobility of large linear and branched DNA in polymer solutions

Near-infrared laser-induced fluorescence detection in capillary electrophoresis

Polymer solutions and entropic‐based systems for double‐stranded DNA capillary electrophoresis and microchip electrophoresis

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