Detection of DNA damage in mussels and sea urchins exposed to crude oil using comet assay

Taban, Ingrid Christina; Bechmann, Renée Katrin; Torgrimsen, Sigfryd; Baussant, Thierry; Sanni, Steinar

doi:10.1016/j.marenvres.2004.03.018

Cited by 73 publications

(19 citation statements)

References 8 publications

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“…For example the Terminal Deoxynucleotidyl transferase-mediated dUTP nick-end labeling assay Impact of near-future ocean acidification on echinoderms 451 (TUNEL) can be used to evaluate apoptosis induced by toxicants on sea urchin larval whole mounts (Filisto et al 2008;Agnello et al 2007). Also, the single-cell microgel electrophoresis assay (Comet assay) has been also used to evaluate DNA damage and repair of dispersed crude oil on sea urchins and mussels (Taban et al 2004). Although adult exposure is feasible with most urchin species, they have been infrequently used in toxicology due to the ready availability of the sensitive and easier sperm and embryo test methods.…”

Section: Echinoderms As Models For Ecotoxicologymentioning

confidence: 99%

Impact of near-future ocean acidification on echinoderms

2010

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As a consequence of increasing atmospheric CO(2), the world's oceans are warming and slowly becoming more acidic (ocean acidification, OA) and profound changes in marine ecosystems are certain. Calcification is one of the primary targets for studies of the impact of CO(2)-driven climate change in the oceans and one of the key marine groups most likely to be impacted by predicted climate change events are the echinoderms. Echinoderms are a vital component of the marine environment with representatives in virtually every ecosystem, where they are often keystone ecosystem engineers. This paper reviews and analyses what is known about the impact of near-future ocean acidification on echinoderms. A global analysis of the literature reveals that echinoderms are surprisingly robust to OA and that important differences in sensitivity to OA are observed between populations and species. However, this is modulated by parameters such as (1) exposure time with rare longer term experiments revealing negative impacts that are hidden in short or midterm ones; (2) bottlenecks in physiological processes and life-cycle such as stage-specific developmental phenomena that may drive the whole species responses; (3) ecological feedback transforming small scale sub lethal effects into important negative effects on fitness. We hypothesize that populations/species naturally exposed to variable environmental pH conditions may be pre-adapted to future OA highlighting the importance to understand and monitor environmental variations in order to be able to to predict sensitivity to future climate changes. More stress ecology research is needed at the frontier between ecotoxicology and ecology, going beyond standardized tests using model species in order to address multiple water quality factors (e.g. pH, temperature, toxicants) and organism health. However, available data allow us to conclude that near-future OA will have negative impact on echinoderm taxa with likely significant consequences at the ecosystem level.

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Section: Echinoderms As Models For Ecotoxicologymentioning

confidence: 99%

Impact of near-future ocean acidification on echinoderms

2010

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“…The study also highlighted the differences in sensitivity between cell types, with gill and digestive gland cells appearing to be the most sensitive target tissues for detecting genotoxic exposure. The Mediterranean mussel (Mytilus galloprovincialis) has also been extensively deployed as a sentinel organism to assess the genotoxic effects of crude oil spills [21][22][23] . Studies have demonstrated the sensitivity of mussels to oil exposure and laboratory studies have clearly linked the total polycyclic aromatic hydrocarbon (TPAHs) content of oils with the level of DNA damage observed 21 .…”

Section: Ecological Relevance Marine Invertebrates (Bivalves)mentioning

confidence: 99%

The Distribution of the MusselMytilusSpecies Along the Norwegian Coast

Brooks

Farmen

2013

Journal of Shellfish Research

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“…Comet assays have been applied to assess the effects of toxicity on different forms of marine organisms (DE BOECK and KIRSCH-VOLDERS, 1997;HARTL et al, 2007;JHA et al 2005;TABAN et al, 2004), including crustaceans (BIHARI and FAFANĐEL, 2004;KUZMICK et al, 2007;ROCHA et al, 2012b). Fluorescent dyes, such as ethidium bromide, DAPI, acridine orange and propididum bromide are the most common dyes used to visualize comets that must be analyzed by fluorescence microscopy before the staining bleaches out.…”

Section: Discussionmentioning

confidence: 99%

Genotoxicity of Shallow Waters Near the Brazilian Antarctic Station "Comandante Ferraz" (Eacf), Admiralty Bay, King George Island, Antarctica

et al. 2015

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Series of biomonitoring surveys were undertaken weekly in February 2012 to investigate the genotoxicity of the shallow waters around the Brazilian Antarctic Station "Comandante Ferraz" (EACF). The comet assay was applied to assess the damage to the DNA of hemocytes of the crustacean amphipods Gondogeneia antarctica collected from shallow waters near the Fuel Tanks (FT) and Sewage Treatment Outflow (STO) of the research station, and compare it to the DNA damage of animals from Punta Plaza (PPL) and Yellow Point (YP), natural sites far from the EACF defined as experimental controls. The damage to the DNA of hemocytes of G. antarctica was not significantly different between sites in the biomonitoring surveys I and II. In survey III, the damage to the DNA of animals captured in shallow waters near the Fuel Tanks (FT) and Sewage Treatment Outflow (STO) was significantly higher than that of the control site of Punta Plaza (PPL). In biomonitoring survey IV, a significant difference was detected only between the FT and PPL sites. Results demonstrated that the shallow waters in front of the station may be genotoxic and that the comet assay and hemocytes of G. antarctica are useful tools for assessing genotoxicity in biomonitoring studies of Antarctic marine coastal habitats.

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Detection of DNA damage in mussels and sea urchins exposed to crude oil using comet assay

Cited by 73 publications

References 8 publications

Impact of near-future ocean acidification on echinoderms

Impact of near-future ocean acidification on echinoderms

The Distribution of the MusselMytilusSpecies Along the Norwegian Coast

Genotoxicity of Shallow Waters Near the Brazilian Antarctic Station "Comandante Ferraz" (Eacf), Admiralty Bay, King George Island, Antarctica

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