Detection of disseminated lung cancer cells in regional lymph nodes by assay of CK19 reverse transcriptase polymerase chain reaction and its clinical significance

Ge, Ming; Wu, Qing; Wang, Mei; Zhang, Yu Hong; Li, Liang Bin

doi:10.1007/s00432-005-0009-0

Cited by 15 publications

(12 citation statements)

References 19 publications

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“…However, even in early stages, the 5-year survival rate of N0 patients remains at only 60-70% after complete resection of the primary tumor (7,17). This suggests that tumor cell dissemination occurs early and occult micrometastases or single disseminated tumor cells (DTC), which are not discovered by conventional histopathologic methods, may be present in the lymph nodes at the time of surgery (6,16,18,22,26). Detection of these cells might potentially improve clinical lymph node staging and help to identify patients who could benefit from adjuvant or neoadjuvant therapy.…”

Section: Introductionmentioning

confidence: 98%

Lymph Node Micrometastases in Lung Cancer

Effenberger¹,

Sienel

Pantel³

2007

Cancer Metastasis and the Lymphovascular System: Basis for Rational Therapy

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Section: Introductionmentioning

confidence: 98%

Lymph Node Micrometastases in Lung Cancer

Effenberger¹,

Sienel

Pantel³

2007

Cancer Metastasis and the Lymphovascular System: Basis for Rational Therapy

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“…As a molecular biology-based technique, it has been shown to be useful in detecting lymph node metastases. This method shows a higher sensitivity for revealing minor tumour deposits in lymph nodes compared to conventional histopathology [15][16][17]. However, this method is not yet used in routine clinical practice, due to its complexity and time-consuming nature.…”

Section: Introductionmentioning

confidence: 99%

Badanie metodą jednostopniowej amplifikacji kwasu nukleinowego węzłów chłonnych w raku brodawkowatym tarczycy — porównanie z badaniem histopatologicznym i badaniem PCR w czasie rzeczywistym

Kaczka

Fendler

Borowiec

et al. 2015

Endokrynologia Polska

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First one-step nucleic acid amplification testing in papillary thyroid cancer lymph nodes -a comparison with histopathology and real-time PCRBadanie metodą jednostopniowej amplifikacji kwasu nukleinowego węzłów chłonnych w raku brodawkowatym tarczycy -porównanie z badaniem histopatologicznym i badaniem PCR w czasie rzeczywistym AbstractIntroduction: The significance of lymph node metastases and the optimal extent of lymphadenectomy remain matters of controversy in papillary thyroid cancer. This study was designed to assess the feasibility and reliability of OSNA and real-time PCR for CK19 and TG mRNA in papillary thyroid cancer lymph nodes evaluation compared to standard histopathology. Material and methods: Each of 92 randomised lymph nodes from 32 papillary thyroid cancer patients were divided into representative parts and assessed using the three studied methods. Results: Eighteen (19.6%) lymph nodes from ten (31.3%) patients were positive according to histopathology. When the cut-off value distinguishing metastatic from non-metastatic lymph nodes in the OSNA assay was set at 250 copies per microlitre, the results were positive in 16 (17.4%) lymph nodes from 11 (34.4%) patients. Twenty three (25%) lymph nodes were tested positive in real-time PCR for TG mRNA. Real-time PCR for CK19 mRNA was positive in 18 (19.6%) lymph nodes from 13 (40.6%) patients. No statistically significant differences were noted between the diagnostic accuracy of either molecular method compared to the histopathological examination (p = 0.81). Overall, 20 positive molecular biology results were noted in patients with negative histopathology results. Conversely, in 18 lymph nodes, despite a metastasis finding in histopathology, at least one molecular test yielded a negative result. Conclusions: It was revealed that OSNA is a reliable technique for the evaluation of lymph node metastases in papillary thyroid cancer. This method was shown to have equivalent accuracy to histopathology and real-time PCR. (Endokrynol Pol 2014; 65 (6): 422-430)

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“…Some molecular examinations used for the assessment of lymph node status have shown higher positivity rates when compared to routine HE staining as well as immunohistochemistry. Nowadays lymph node metastases are diagnosed more and more often by molecular assays and immunohistochemistry [17][18][19][20][21][22][23][24][25]. The data of these studies may help to find more sensitive methods for the early detection of cell dissemination and may refine risk groups that might benefit from more extensive surgery or adjuvant therapy.…”

Section: Introductionmentioning

confidence: 99%

Lymph node metastases in papillary thyroid cancer detected by quantitative real-time polymerase chain reaction for thyroglobulin and cytokeratine-19

Kaczka¹,

Fendler²,

Borowiec³

et al. 2013

pjp

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Papillary thyroid cancer (PTC) metastases in the lymph nodes (LNs) were detected by real-time polymerase chain reaction (PCR) for TG and cytokeratin 19 (CK19), and the obtained results were compared with histopathology. 107 LNs from 34 PTC patients were divided into four blocks by a special cutting device -2 for histopathology, while the other 2 were tested by quantitative real-time PCR. Metastases were detected in 20 nodes from 10 (29.4%) patients. TG and CK19 expression levels differed vastly between nodes with and without metastatic cells. ddCt of TG in the genetic material extracted from N0 nodes was 9.97 ±4.20, while in nodes with metastases ddCt was 0.91±4.20 (p < 0.0001). Cytokeratin 19 showed similar results with expression level (ddCt) in N0 nodes of 10.96 ±2.58 vs. 7.73 ±3.63 in nodes with metastases (p < 0.0001). Evaluation of the utility of both parameters showed efficient differentiation of node involvement in the case of TG, with area under the ROC curve (AUC) equal to 0.91 (95% CI: 0.85-0.96). Cytokeratin 19 also allowed for a degree of differentiation but its diagnostic efficacy was lower (AUC 0.76, 95% CI: 0.64-0.88). The combined TG and CK19 quantitative real-time PCR could be used to select a previously missed group of patients with nodal involvement undetectable by standard histopathology.

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Detection of disseminated lung cancer cells in regional lymph nodes by assay of CK19 reverse transcriptase polymerase chain reaction and its clinical significance

Cited by 15 publications

References 19 publications

Lymph Node Micrometastases in Lung Cancer

Lymph Node Micrometastases in Lung Cancer

Badanie metodą jednostopniowej amplifikacji kwasu nukleinowego węzłów chłonnych w raku brodawkowatym tarczycy — porównanie z badaniem histopatologicznym i badaniem PCR w czasie rzeczywistym

Lymph node metastases in papillary thyroid cancer detected by quantitative real-time polymerase chain reaction for thyroglobulin and cytokeratine-19

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