Detection of DGAT1 gene polymorphism and its effect on selected biochemical indicators in dairy cows after calving

Lešková, L.; Bauer, Miroslav; Chrenek, Peter; Lacková, Z.; Soročinová, J.; Petrovič, Vladimír; Kováč, G.

doi:10.2754/avb201382030265

Cited by 7 publications

(5 citation statements)

References 13 publications

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“…Accordingly, associations of these genes with FA were reported in previous studies (Bovenhuis et al, 2016;Palombo et al, 2018;Sanchez et al, 2018;Cruz et al, 2019). DGAT1 is a major gene influencing inflammatory response and lipid metabolism and encodes an enzyme that catalyzes the final step of triacylglycerol synthesis (Cases et al, 1998), and significantly affected energy metabolism in early lactation in high yielding cows (Lešková et al, 2013).…”

Section: Potential Candidate Genes For Heat Stress Response Across Fa...mentioning

confidence: 75%

Genome-wide associations for heat stress response suggest potential candidate genes underlying milk fatty acid composition in dairy cattle

Bohlouli

Halli

Yin

et al. 2022

Journal of Dairy Science

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Contents of milk fatty acids (FA) display remarkable alterations along climatic gradients. Detecting candidate genes underlying such alterations might be beneficial for the exploration of climate sensitivity in dairy cattle. Consequently, we aimed on the definition of FA heat stress indicators, considering FA breeding values in response to temperature-humidity index (THI) alterations. Indicators were used in GWAS, in ongoing gene annotations and for the estimation of chromosome-wide variance components. The phenotypic data set consisted of 39,600 test-day milk FA records from 5,757 first-lactation Holstein dairy cows kept in 16 large-scale German cooperator herds. The FA traits were C18:0, polyunsaturated fatty acids (PUFA), saturated fatty acids (SFA), and unsaturated fatty acids (UFA). After genotype quality control, 40,523 SNP markers from 3,266 cows and 930 sires were considered. Meteorological data from the weather station in closest herd distance were used for the calculation of maximum hourly daily THI, which were allocated to 10 different THI classes. The same FA from 3 stages of lactation were considered as different, but genetically correlated traits. Consequently, a 3-trait reaction norm model was used to estimate genetic parameters and breeding values for FA along THI classes, considering either pedigree (A) or genomic (G) relationship matrices. Deregressed proofs and genomic estimated breeding values at the intermediate THI class 5 and at the extreme THI class 10 were used as pseudophenotypes in ongoing genomic analyses for thermoneutral (TNC) and heat stress conditions (HSC), respectively. The differences in de-regressed proofs and in genomic estimated breeding values from both THI classes were pseudophenotypes for heat stress response (HSR). Genetic correlations between the same FA under TNC and HSC were smallest in the first lactation stage and ranged from 0.20 for PUFA to 0.87 for SFA when modeling with the A matrix, and from 0.35 for UFA to 0.86 for SFA when modeling with the G matrix. In the first lactation stage, larger additive genetic variances under HSC compared with TNC indicate climate sensitivity for C18:0, PUFA, and UFA. Climate sensitivity was also reflected by pronounced chromosome-wide genetic variances for HSR of PUFA and UFA in the first stage of lactation. For all FA under TNC, HSC, and HSR, quite large genetic variance proportions were explained by BTA14. In GWAS, 30 SNP (within or close to 38 potential candidate genes) overlapped for HSR of the different FA. One unique potential candidate gene (AMFR) was detected for HSR of PUFA, 15 for HSR of SFA (ADGRB1,

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Section: Potential Candidate Genes For Heat Stress Response Across Fa...mentioning

confidence: 75%

Genome-wide associations for heat stress response suggest potential candidate genes underlying milk fatty acid composition in dairy cattle

Bohlouli

Halli

Yin

et al. 2022

Journal of Dairy Science

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“…However, there were no previous indications of association of the identified SNP in this region with ketosis in previous studies. The enzyme produced by the DGAT1 gene (acyl-CoA: diacylglycerol acyltransferase) catalyzes the last step of triacyglyceride (TAG) synthesis in the liver and can significantly influence the energy metabolism in dairy cows [56]. It has been shown that excessive accumulation of TAG in liver and oxidative stress as a result of altered lipid metabolism early in lactation can increase the risk of metabolic disease including fatty liver and ketosis in dairy cattle [56].…”

Section: Discussionmentioning

confidence: 99%

“…The enzyme produced by the DGAT1 gene (acyl-CoA: diacylglycerol acyltransferase) catalyzes the last step of triacyglyceride (TAG) synthesis in the liver and can significantly influence the energy metabolism in dairy cows [56]. It has been shown that excessive accumulation of TAG in liver and oxidative stress as a result of altered lipid metabolism early in lactation can increase the risk of metabolic disease including fatty liver and ketosis in dairy cattle [56]. Estimates of genetic correlations between 305-d milk yield and ketosis were found to be mostly unfavorable, with estimates ranging from 0.00 to 0.77 [57–59].…”

Section: Discussionmentioning

confidence: 99%

Genome-wide association analysis for β-hydroxybutyrate concentration in Milk in Holstein dairy cattle

et al. 2019

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Background Ketosis in dairy cattle has been shown to cause a high morbidity in the farm and substantial financial losses to dairy farmers. Ketosis symptoms, however, are difficult to identify, therefore, the amount of ketone bodies (mainly β-hydroxybutyric acid, BHB) is used as an indicator of subclinical ketosis in cows. It has also been shown that milk BHB concentrations have a strong correlation with ketosis in dairy cattle. Mid-infrared spectroscopy (MIR) has recently became a fast, cheap and high-throughput method for analyzing milk components. The aim of this study was to perform a genome-wide association study (GWAS) on the MIR-predicted milk BHB to identify genomic regions, genes and pathways potentially affecting subclinical ketosis in North American Holstein dairy cattle. Results Several significant regions were identified associated with MIR-predicted milk BHB concentrations (indicator of subclinical ketosis) in the first lactation (SCK1) and second and later lactations (SCK2) in Holstein dairy cows. The strongest association was located on BTA6 for SCK1 and BTA14 on SCK2. Several SNPs on BTA6 were identified in regions and variants reported previously to be associated with susceptibility to ketosis and clinical mastitis in Jersey and Holstein dairy cattle, respectively. One highly significant SNP on BTA14 was found within the DGAT1 gene with known functions on fat metabolism and inflammatory response in dairy cattle. A region on BTA6 and three SNPs on BTA20 were found to overlap between SCK1 and SCK2. However, a novel region on BTA20 (55–63 Mb) for SCK2 was also identified, which was not reported in previous association studies. Enrichment analysis of the list of candidate genes within the identified regions for MIR-predicted milk BHB concentrations yielded molecular functions and biological processes that may be involved in the inflammatory response and lipid metabolism in dairy cattle. Conclusions The results of this study confirmed several SNPs and genes identified in previous studies as associated with ketosis susceptibility and immune response, and also found a novel region that can be used for further analysis to identify causal variations and key regulatory genes that affect clinical/ subclinical ketosis. Electronic supplementary material The online version of this article (10.1186/s12863-019-0761-9) contains supplementary material, which is available to authorized users.

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“…Diacylglycerol Acyltransferase-1 (DGAT1) gene is one of the functional candidate genes affecting milk composition traits (Juhlin et al, 2012). The cattle DGAT1 gene is located on chromosome 14 and contains 17 exons ( (Lešková et al, 2013). The dinucleotide change (AA/GC) at positions 10433 and 10434 (rs AJ318490.1) in exon 8 leads to a non-conservative substitution of Lysine by Alanine at position 232 and has been shown to affect milk yield strongly and milk composition in Italian Holsteins (Bobbo, et al, 2018), White Fulani and Borgou cattle breeds (Houaga et al, 2017) and Holstein, Simmental and Brown Swiss cattle breeds in Croatia (Dokso et al, 2015).…”

Section: Thementioning

confidence: 99%

Dgat1 Gene Polymorphism and Its Relationships With Cattle Milk Yield and Chemical Composition

Faraj¹,

Ayied²,

Seger³

2020

PTQ

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Several polymorphisms in different gene loci have been noted to affect production traits such as milk yield and milk composition. The present study aimed to determine the allelic and genotypic frequency of the DGAT1 gene and evaluate the associations between DGAT1 genetic variants and milk yield and its chemical composition of local Iraqi cattle. Blood samples from 100 cows were obtained for DNA isolation. The primer used in this study amplified 411-bp fragments at exon 8 of the DGAT1 gene. DNA sequencing methods were applied to detect single nucleotide polymorphism of the DGAT1 gene in 100 cows. The nucleotide sequences of exon 8 of the DGAT1 gene were registered for local Iraqi cattle in the National Center for Biotechnology Information (NCBI), DNA Data Bank of Japan (DDBJ), and the European Nucleotide Archive (ENA) under the following accession numbers (LC492073 and LC492074). The results showed the presence of two polymorphic sites leading to the construction of 2 different haplotypes in the cow. Haplotype diversity was 0.536, while nucleotide diversity was 0.0031. Two single-nucleotide polymorphism (SNP) loci of the DGAT1 gene were detected, namely A10433G (A/G) and A10434C (A/C). The resulting of this mutation changes lysine to alanine substitution at position 232 (A232K mutation) of amino acid sequence. Geneious software V. 2020.0.4 was used to detect genotypes of the DGAT1 gene, as the sequence alignment showed the presence of three genotypes. The genotypic frequencies of KK, KA, and AA were 0.40, 0.30, and 0.30, respectively. Frequencies of K and A alleles were 0.60 and 0.40, respectively. The KK genotype was significantly (P 0.05) associated with higher fat yield. Therefore, the DGAT1 gene could serve as a genetic marker for the selection of fat yield in cows.

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Detection of DGAT1 gene polymorphism and its effect on selected biochemical indicators in dairy cows after calving

Cited by 7 publications

References 13 publications

Genome-wide associations for heat stress response suggest potential candidate genes underlying milk fatty acid composition in dairy cattle

Genome-wide associations for heat stress response suggest potential candidate genes underlying milk fatty acid composition in dairy cattle

Genome-wide association analysis for β-hydroxybutyrate concentration in Milk in Holstein dairy cattle

Dgat1 Gene Polymorphism and Its Relationships With Cattle Milk Yield and Chemical Composition

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