Detection of deletions in 1q25, 1p36 and 1pTEL and chromosome 17 aneuploidy in oral epithelial dysplasia and oral squamous cell carcinoma by fluorescence in situ hybridization (FISH)

Rabenhorst, Sílvia Helena Barem; Osterne, Rafael Lima Verde; Nonaka, Cassiano Francisco Weege; Rodrigues, Andre Montezuma Sales; Nogueira, Renato Luiz Maia; Burbano, Rommel Rodrı́guez; Cavalcante, Roberta Barroso

doi:10.1016/j.oraloncology.2021.105221

Cited by 3 publications

(5 citation statements)

References 42 publications

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“…[ 1 - 5 ] From the diagnosed OPMD, samples were collected for analysis by either biopsy ( n = 21) or brush cytology ( n = 8), while one of the included articles used micro biopsy[ 6 ] for the analysis. While considering the method used for ploidy analysis, two articles used FISH,[ 2 ] 16 articles used FC or high-resolution FC,[ 1 , 5 - 19 ] 11 used IC,[ 1 , 3 , 4 , 20 - 27 ] one used static cytometry,[ 28 ] and a single article used both DNA FC and IC to assess the sensitivity of the two methods. [ 29 ]…”

Section: R Esultsmentioning

confidence: 99%

“…Eighteen of the included articles assessed almost all OPMD. [ 2 , 3 , 4 , 6 - 10 , 12 , 13 , 16 - 18 , 21 - 23 , 30 ] ranging from oral leukoplakia (OL), erythroplakia (EP), oral lichen planus (OLP), proliferative verrucous leucoplakia (PVL), and dysplastic lesions. Eight articles included only patients with OL and its types[ 1 , 5 , 15 , 19 , 20 , 26 , 27 , 29 ] and four articles analyzed patients with only OLP.…”

Section: R Esultsmentioning

confidence: 99%

“…Eight articles included only patients with OL and its types[ 1 , 5 , 15 , 19 , 20 , 26 , 27 , 29 ] and four articles analyzed patients with only OLP. [ 2 , 11 , 14 , 25 ] There was a clear female predilection in most of the studies where smoking was not considered as a main causation factor.…”

Section: R Esultsmentioning

confidence: 99%

“…From the above studies, it was concluded that DNA ploidy was an independent bio-marker for malignant transformation. [ 2 , 4 , 5 , 7 , 12 , 16 , 17 , 20 , 22 , 24 , 27 , 29 , 30 ] Few concluded that DNA ploidy status can be used to predict malignant transformation while taking other markers such as dysplasia, genomic damage, and DI into account. [ 1 , 6 , 10 , 13 , 14 , 16 , 26 ] Tongue OPMD were more frequently associated with DNA aneuploidy than any other locations.…”

Section: R Esultsmentioning

confidence: 99%

“…DNA ploidy is one such important biomarker. [ 1 , 2 ] These are markers of genomic instability and promotes tumor development and progression as they are the fundamental processes in dysplasia and carcinogenesis. These genomic instabilities can be detected in early stages of cancer development and allow initiation of early targeted treatment contributing to good prognosis and secondary prevention.…”

Section: Introductionmentioning

confidence: 99%

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Assessment of Ploidy Status in Oral Potentially Malignant Disorders – A Systematic Review

Annapoorani,

Gururaj,

Balambigai

et al. 2023

Journal of Pharmacy and Bioallied Sciences

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Malignant and potentially malignant epithelial lesions are often associated with various abnormalities such as epithelial dysplasia, abnormal DNA content, loss of heterozygosity, and chromosomal number aberrations. Screening and early detection of such abnormalities facilitates proper care and also helps to prevent further progression of potentially malignant lesions to malignancy. In such way, the presence of DNA aneuploidy in oral potentially malignant disorders (OPMDs) may serve as an indicator for the malignant transforming potential. Various assessment methods have been proposed to find the DNA ploidy status of cells. This current systematic review is mainly designed to assess the importance of ploidy status in OPMD while measuring the feasibility of using this biomarker for evaluating the hazard of malignant transformation. As an upshot of this systematic review, we can conclude that use of DNA ploidy status can serve as an independent bio-marker for predicting the malignant transformation of lesions. Furthermore, as a future scope the use of DNA ploidy analysis in normal mucosa of smokers will help to assess the malignancy risk and this technique might also help to predict the genetic predisposition of patients with malignancy.

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Section: R Esultsmentioning

confidence: 99%

Section: R Esultsmentioning

confidence: 99%

Section: R Esultsmentioning

confidence: 99%

Section: R Esultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Assessment of Ploidy Status in Oral Potentially Malignant Disorders – A Systematic Review

Annapoorani,

Gururaj,

Balambigai

et al. 2023

Journal of Pharmacy and Bioallied Sciences

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A bibliometric analysis of the papers on oral potentially malignant disorder in Oral Oncology

Yang

et al. 2022

Oral Oncology

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Novel Driver Strength Index highlights important cancer genes in TCGA PanCanAtlas patients

Belikov

Otnyukov

Vyatkin

et al. 2021

Preprint

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Elucidating crucial driver genes is paramount for understanding the cancer origins and mechanisms of progression, as well as selecting targets for molecular therapy. Cancer genes are usually ranked by the frequency of mutation, which, however, does not necessarily reflect their driver strength. Here we hypothesize that driver strength is higher for genes that are preferentially mutated in patients with few driver mutations overall, because these few mutations should be strong enough to initiate cancer. We propose a formula to calculate the corresponding Driver Strength Index (DSI), as well as the Normalized Driver Strength Index (NDSI), the latter completely independent of the overall gene mutation frequency. We validate these indices using the largest database of human cancer mutations - TCGA PanCanAtlas, multiple established algorithms for cancer driver prediction (2020plus, CHASMplus, CompositeDriver, dNdScv, DriverNet, HotMAPS, IntOGen Plus, OncodriveCLUSTL, OncodriveFML) and four custom computational pipelines that integrate driver contributions from SNA, CNA and aneuploidy at the patient-level resolution. We demonstrate that NDSI provides substantially different rankings of genes as compared to DSI and frequency approach. For example, NDSI highlighted the importance of guanine nucleotide-binding protein subunits GNAQ, GNA11, GNAI1, GNAZ and GNB3, General Transcription Factor II family members GTF2I and GTF2F2, as well as fibroblast growth factor receptors FGFR2 and FGFR3. Intriguingly, NDSI prioritized CIC, FUBP1, IDH1 and IDH2 mutations, as well as 19q and 1p chromosome arm losses, that comprise characteristic molecular alterations of gliomas. KEGG analysis shows that top NDSI-ranked genes comprise PDGFRA-GRB2-SOS2-HRAS/NRAS-BRAF pathway, GNAQ/GNA11-HRAS/NRAS-BRAF pathway, GNB3-AKT1-IKBKG/GSK3B/CDKN1B pathway and TCEB1-VHL pathway. NDSI does not seem to correlate with the number of protein-protein interactions. We share our software to enable calculation of DSI and NDSI for outputs of any third-party driver prediction algorithms or their combinations.

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Detection of deletions in 1q25, 1p36 and 1pTEL and chromosome 17 aneuploidy in oral epithelial dysplasia and oral squamous cell carcinoma by fluorescence in situ hybridization (FISH)

Cited by 3 publications

References 42 publications

Assessment of Ploidy Status in Oral Potentially Malignant Disorders – A Systematic Review

Assessment of Ploidy Status in Oral Potentially Malignant Disorders – A Systematic Review

A bibliometric analysis of the papers on oral potentially malignant disorder in Oral Oncology

Novel Driver Strength Index highlights important cancer genes in TCGA PanCanAtlas patients

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