Detection of decay-accelerating factor in stool specimens of patients with colorectal cancer

Complement in Cancer and Cancer Immunotherapy

“…Breast cancers seem to have a moderate to high expression of all three mCRegs in both tumours and their associated cell lines and even secrete soluble CD55 and CD59 Hakulinen and Meri 1994;Hofman et al 1994;Niehans et al 1996;Thorsteinsson et al 1998). Colorectal tumours have also been shown to express all three mCRegs (membrane and soluble variants) in both cell lines and primary tumours (Gelderman et al 2002a;Mizuno et al 1995;Niehans et al 1996;Thorsteinsson et al 1998). For a comprehensive table of mCRegs expression in primary tumours and tumour cell lines see Fishelson et al (2003).…”

Polymorphic expression of decay‐accelerating factor in human colorectal cancer

“…Because o ‐linked glycosylation of DAF is important for the expression and stability of DAF on the cell surface, 6 the variability of o ‐linked sugars may indicate differences in the steady‐state level of colorectal cancer DAF on the cell surface, which might cause modulation of complement‐mediated host immune responses to cancer cells. We have previously shown that increased amounts of DAF were released into stools of patients with colorectal cancer compared with normal subjects 5 . Thus, the stability on the cell surface might influence the amount of DAF present in the stools of colorectal cancer patients.…”

“…Expression of DAF was enhanced in colorectal cancer and DAF was intensely stained on the luminal surface and in the lumen of cancer glands. In addition, we have found that the stools of patients with colorectal cancer contained increased amounts of DAF 5 . o‐ Linked glycosylation of DAF is important for the release of DAF protein from the cancer cell surface into stools because it is related to the surface expression and stability of DAF on the cell surface 6 .…”

Transferrin Dipstick as a Potential Novel Test for Colon Cancer Screening: A Comparative Study With Immuno Fecal Occult Blood Test

“…For example, a "hydrated fecal occult blood test" was developed to improve the sensitivity of Guaiac test, but it was then eliminated due to its high falsepositive rate (16). Other biomarkers, such as "fecal microalbumin (17)," "fecal decay-accelerating factor (18)," "fecal calprotectin (19)," and "fecal tumor associated antigen (20)" have also been studied but without significant improvement of detecting colon cancer. IFOBT certainly offers several advantages over conventional chemicalbased assay, including higher sensitivity, not required to restrict certain food types that may cause false positiveness, etc.…”