Detection of cytomegalovirus‐specific igm in renal transplant recipients

McMahon, Cheryl A.; Lentz, Ellyn B.; Dock, Nancy L.; Lamberson, Harold V.; Forbes, Betty A.; Reinitz, Emanuel R.

doi:10.1002/jcla.1860030607

Cited by 7 publications

(7 citation statements)

References 29 publications

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“…Infection is detected by the isolation of HCMV from clinical specimens, such as urine, bronchoalveolar lavage fluid, induced sputum, and blood buffy coat [3]. Serologic diagnosis of infection is performed mostly by complement fixation (CF) tests and enzyme-linked immunoassays (ELISA), which allow detection of HCMV-specific immunoglobulin of IgG, IgM and IgA classes [4][5][6][7][8][9][10]. Even with the most sensitive tests, a proportion of congenitally or neonatally infected infants have been found to produce either no HCMV IgM antibodies or only very small amounts [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Improvement of serological diagnosis of human cytomegalovirus infection in renal transplant recipients by testing for specific immunoglobulin E by ELISA

Weber¹,

Stemmler²,

Ernst³

et al. 1993

Infection

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The kinetics of human cytomegalovirus (HCMV)-specific immunoglobulin E (IgE), M (IgM), A (IgA) and G (IgG) were studied in 421 sera obtained from 19 renal allograft recipients by enzyme-linked immunosorbent assay (ELISA). Cytomegalic inclusion disease (CID) occurred in 11 (57.9%) patients. HCMV infection was diagnosed in all (100%) of these patients by testing for specific IgE. In contrast, increased levels of IgM and IgA class antibody against HCMV were detected in only 45.5% and 18.2% patients suffering from primary or recurrent HCMV infection, respectively. Concerning the time interval between the onset of clinical symptoms and the first positive test, no significant differences in the kinetics of HCMV-specific immunoglobulins E, M, A and G were observed. Elevated specific IgE levels persisted for longer time intervals than the other immunoglobulin classes. As shown by the present study, specific IgE proved to be a more reliable serologic marker than IgM and IgA for the serologic detection of HCMV infection in renal allograft recipients.

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Section: Introductionmentioning

confidence: 99%

Improvement of serological diagnosis of human cytomegalovirus infection in renal transplant recipients by testing for specific immunoglobulin E by ELISA

Weber¹,

Stemmler²,

Ernst³

et al. 1993

Infection

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“…Detection of CMV-specific IgM is most commonly effected using preparations of the virus or viral lysate in an enzyme-linked immunosorbent assay (ELISA) [18][19][20][21][22][23][24]. The key serological targets for detection of CMV-specific IgM include both structural (pUL32, pUL83, pUL80a) [25][26][27][28][29], and nonstructural (pUL57, pUL44) [24,[30][31][32] viral proteins.…”

Section: Serological Advancesmentioning

confidence: 99%

New Advances in the Diagnosis of Congenital Cytomegalovirus Infection

Lazzarotto¹,

Varani²,

Gabrielli³

et al. 1999

Intervirology

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With the advances in anticytomegalovirus (anti-CMV) serology, the new recombinant IgM tests seem likely to become the screening tests for pregnant women whose prepregnancy serological status for CMV is unknown. When a woman is found to be IgM-positive, further diagnostic evaluation focused on determining whether this is due to a primary infection should be carried out. Maternal primary infections that were difficult to determine until a few years ago unless documented by seroconversion can now be readily diagnosed from the presence of low-avidity anti-CMV antibody which persists for approximately 20 weeks after primary infection. In primarily infected mothers prenatal diagnosis can be performed between 21 and 23 weeks of gestation, and the amniotic fluid (AF) represents the pathological material of choice to determine intrauterine virus transmission. In AF, the virus can be detected by culture and/or PCR. Both procedures differentiate uninfected from infected fetuses, but cannot predict fetal outcome. The determination of the viral load in AF carried out by quantitative PCR is more promising and could represent an important starting point for preemptive fetal therapy.

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“…However, IgM antibodies may also be elevated in the serum of patients with reactivation of a persistent viral infection [30][31][32][33][34][35][36][37][38][39][40] and chronic spirochete infection 41 . For our study we chose to test only RF-negative sera in order to exclude possible interference by IgM RF in the serological assay 42 .…”

Section: Discussionmentioning

confidence: 99%