Detection of cytokines in bovine colostrum

Hagiwara, Kaoru; Kataoka, Satoshi; Yamanaka, Hitoki; Kirisawa, Rikio; Iwai, Hiroshi

doi:10.1016/s0165-2427(00)00213-0

Cited by 140 publications

(112 citation statements)

References 23 publications

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“…Consistent with the constitutive mRNAexpression of proinflammatory cytokines in mammary tissues [60], the proinflammatory cytokine production here was also seen in mammary tissues obtained from sows before inoculation with E. coli. These findings are consistent with previous studies in rats, humans as well as in cows where some baseline constitutive production of cytokines takes place in normal mammary glands [7,23,56]. Similarly, proinflammatory cytokines have also been found in serum/plasma collected from healthy pregnant sows [59] as well as pregnant women [4,24,57].…”

Section: Discussionsupporting

confidence: 92%

“…These small proteins, including interleukin-1 beta (IL-1β), IL-6, IL-8, and tumor necrosis fac-component of a paracrine/autocrine communication network in the mammary gland throughout the different stages of pregnancy and parturition [25,44]. It should be noted that a low constitutive production of some of these cytokines may take place in healthy individuals [22,23,55].…”

Section: Introductionmentioning

confidence: 99%

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Morphometric analysis of proinflammatory cytokines in mammary glands of sows suggests an association between clinical mastitis and local production of IL-1beta, IL-6 and TNF-alpha

et al. 2007

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-Twelve healthy primiparous sows received intramammary inoculation with Escherichia coli (serotype O127) during the 24-h period preceding parturition. Mammary gland biopsy samples were taken immediately before inoculation (0 h) and from the inoculated and the contralateral noninoculated glands 24 h after inoculation. The analyses of interleukin-1 beta (IL-1β), IL-6, IL-8, and tumor necrosis factor alpha (TNF-α) by immunohistochemistry revealed that the production of these proinflammatory cytokines significantly increased in the inoculated mammary glands of sows that developed clinical signs of mastitis (affected group, n = 4) 24 h after inoculation. This was also true for IL-8 in the inoculated mammary glands of sows that did not develop clinical signs of mastitis (nonaffected group, n = 8). Sows that developed clinical signs of mastitis displayed significantly lower constitutive production of IL-1β than did sows that remained clinically healthy. The data indicate that the development of clinical symptoms of coliform mastitis in the sow is associated with a locally increased proinflammatory cytokine production in response to intramammary E. coli infection.cytokine / immunohistochemistry / mastitis / pig / E. coli

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Morphometric analysis of proinflammatory cytokines in mammary glands of sows suggests an association between clinical mastitis and local production of IL-1beta, IL-6 and TNF-alpha

et al. 2007

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“…Several components within colostrum can influence the immunological development of the offspring. The presence of several pro-inflammatory (IL-1β, TNF-α) and anti-inflammatory (IL-1Ra, TNF-α receptor I and II, and IL-10) molecules has been widely demonstrated in bovine colostrum [12]. These factors usually decrease in mature milk, and are likely to be involved in the development of the immature immune system of neonates.…”

Section: Discussionmentioning

confidence: 99%

Identification of the bovine α1-acid glycoprotein in colostrum and milk

et al. 2005

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-α1-acid glycoprotein (AGP) is an immunomodulatory protein expressed by hepatocytes in response to the systemic reaction that follows tissue damage caused by inflammation, infection or trauma. This paper presents the detection of bovine AGP (boAGP) in mammary secretions (colostrum and milk) and mammary gland tissue. Bovine AGP was detected by Western blotting in all the samples analysed, and could be quantified in colostrum at 162 (± 63.7) µg/mL and 114.5 (± 67.8) µg/mL during the first 12 h and 24 h respectively. In mature milk, the boAGP concentration clearly decreased and was no longer detectable using the Radial Immunodiffusion (RID) technique. The concentration of mature milk boAGP was therefore semi-quantified using an anion-exchange chromatographic procedure that allowed the concentration of the protein to be determined. The presence of AGP in bovine milk was confirmed by the internal sequence analysis performed following purification to homogeneity of the protein from milk. The concentration of AGP in bovine milk with low SCC (< 250 000) was very similar to that from bovine milk with high SCC (> 250 000). In order to investigate the origin of AGP in bovine milk, a search for mRNA was carried out in somatic cells and mammary gland tissue: mRNA expression of the boAGP gene was detected in mammary gland tissue, but not in somatic cells. Finally, the cDNA sequence of the boAGP was determined, and is hereby presented. α1-acid glycoprotein / mastitis / colostrum / milk

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“…Lactose increases from 2.7 to 4.7%. It is known for its laxative property [13] due to a thermoresistant component causing fast removal of the "meconium" from the intestinal tracts of the offspring [10].…”

Section: Introductionmentioning

confidence: 99%

“…Several studies which were reviewed by Scammell [35] also provide evidence of the efficacy of colostrum in human clinical applications such as treatment of acute infant gastroenteritis [11] and chronic refractory diarrhoea due to Cryptosporidium in AIDS patients [29,31]. More recently, a lot of attention has been paid to colostrum because of its exceptional content in (i) many growth factors such as EGF (Epithelial Growth Factor), IGF (Insulin Growth Factor), NGF (Nerve Growth Factor) and TGF-β 2 (Transforming Growth Factor), which play a critical role in the regulation and differentiation of a variety of cells [15,16,[26][27][28] and (ii) cytokines [10] with particular interest in a proline-rich polypeptide which was shown to improve the outcome of Alzheimer's Disease patients with mild to moderate dementia [18]. Consequently, colostrum and its derivatives may be of a great interest to the cosmeceutical and nutraceutical markets.…”

Section: Introductionmentioning

confidence: 99%

Preparation of serocolostrum by membrane microfiltration

Piot¹,

Fauquant²,

Madec³

et al. 2004

Lait

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-Treatment of bovine, equine and caprine colostrum by membrane microfiltration with a pore size of 0.1 µm was studied in order to obtain a specific separation of whey colostrum components. These components are most interesting for imparting passive immunity and positive physiological action to newborn mammals. From the carrying out of 80 kg batches of colostrum, the microfiltration equipment used allowed the recovery of at least 80% of the IgG and other minor whey proteins in the microfiltrate. This liquid, named serocolostrum, is crystal clear, free of blood and somatic cells as well as fat globules and casein micelles, and it has a high hygienic and bacteriological quality (less than 10 CFU·mL -1 ). Specific concentration of the serocolostrum IgG proteins by high molecular weight cut-off (MWCO: 100 kg·mol -1 ) membrane ultrafiltration was also studied in order to obtain purified IgG products suitable for preliminary animal trials. Purity (IgG/TS) as high as 90% was obtained. The use of a lower MWCO UF membrane (8 kg·mol -1 ) allowed concentrations of growth factors (TGF-β and IGF-1) but the observed separation did not agree with the data of the literature, i.e. a binding of IGF-1 with a 45 kg·mol -1 protein.Colostrum / membrane / microfiltration / ultrafiltration / serocolostrum / IgG / TGF-β / IGF-1 Résumé -Préparation de « sérocolostrum » par microfiltration sur membrane. La mise en contact de colostrums de vache, jument et chèvre avec une membrane de microfiltration ayant un diamètre de pores de 0,1 µm permet de réaliser une séparation spécifique des composants du sérum, composants d'intérêt pour obtenir une immunisation passive du jeune mammifère. Avec l'équipe-ment MF pilote utilisé, la mise en oeuvre de 80 kg de colostrum conduit à une récupération d'au moins 80 % des IgG et des protéines mineures dans le microfiltrat. Ce liquide, appelé sérocolostrum, est limpide, il ne contient ni cellule sanguine, ni cellule somatique, ni globule gras, ni micelle de caséine. Sa qualité bactériologique est particulièrement élevée (moins de 10 CFU·mL -1 ). La concentration spécifique des protéines IgG, contenues dans le sérocolostrum par ultrafiltration sur membrane (ayant un pouvoir de coupure élevé, 100 kg·mol -1 ) a également été étudiée avec pour objectif l'obtention de produits enrichis en IgG (pureté d'au moins 90 %) pouvant être utilisés en expérimentations sur les jeunes animaux. Grâce à l'emploi de membranes UF de plus petit pouvoir de coupure (8 kg·mol -1 ), la concentration spécifique des facteurs de croissance (TGF-β, IGF-1) a pu être étudiée. La perméation déterminée pour l' IGF-1 n'était pas en accord avec les données de la littérature qui précise que dans le colostrum et dans le lait, ce facteur de croissance serait lié avec une protéine de 45 kg·mol -1 .Colostrum / membrane / microfiltration / ultrafiltration / sérocolostrum / IgG / TGF-β / IGF-1 * Corresponding author: maubois@rennes.inra.fr 334 M. Piot et al.

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Detection of cytokines in bovine colostrum

Cited by 140 publications

References 23 publications

Morphometric analysis of proinflammatory cytokines in mammary glands of sows suggests an association between clinical mastitis and local production of IL-1beta, IL-6 and TNF-alpha

Morphometric analysis of proinflammatory cytokines in mammary glands of sows suggests an association between clinical mastitis and local production of IL-1beta, IL-6 and TNF-alpha

Identification of the bovine α1-acid glycoprotein in colostrum and milk

Preparation of serocolostrum by membrane microfiltration

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