Detection of Cytokine mRNA in Polyclonally‐, Antigen‐ or Allergen‐Stimulated Mononuclear Cells

Gauchat, Jean‐François; Gauchat, Dominique; Qiu, Gang; Mandallaz, Michèle; Stadler, Beda M.

doi:10.1111/j.1600-065x.1991.tb00582.x

Cited by 7 publications

(6 citation statements)

References 46 publications

(36 reference statements)

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“…expression in patients and conlrols. This is similar to the findings of Gauchat et ai, who reported equivalent expression of iFN-7 mRNA in pokeweed mitogen (PWM) or allergen-stimulated PBMC from monoailergie patients and controls [27]. Contrary to our finding of spontaneous IFN-7 expression in unslimulated eultures from children with AD, however, the latler study did not detect IFN-7 mRNA in unstimulated cultures from allergic patients.…”

Section: Discussionsupporting

confidence: 91%

“…This constitutive expression of IFN-7 mRNA demonstrated for children with AD substantiates our previous finding of IFN-7-containing cells in unstimulated cultures frotn children with AD [9], Taken together, these results are also consistent with the suggestion that dilTcrences in cytokine mRNA levels reflect variations in the number of cytokine-producing cells rather than increased transcription in eaeh eell [26,27]. Both T cells and natural killer (NK) eells have been shown to produce IFN-7 in vitro [28,29], The importance of NK cells as a source of the spontaneous IFN-7 mRNA expression in these children with AD is questionable, however, as NK cell number and function have been reported to be reduced in atopic individuals with AD [30,31].…”

Section: Discussionsupporting

confidence: 90%

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Reduced interferon-gamma (IFN-γ) secretion with increased IFN-γ mRNA expression in atopic dermatitis: evidence for a post-transcriptional defect

Tang

Varigos

Kemp

1994

Clinical and Experimental Immunology

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SUMMARYReduced secretioti of IFN-7 in atopic itidividuals has been implicated iti the pathogenesis of disease, though the mechanisms leading to this reduced secretion have nol been elucidated. As production of IFN-'y has been shown to be predominantly regulated by its rate of transcription, expression of IFN-7 mRNA was examined in atopic children and in age-matched, non-atopic controls by polymerase chain reaction (PCR)-assisted mRNA amplification. Children with atopic dermatitis were found to have constitutive expression of IFN-7 mRNA in freshly isolated peripheral blood mononuclear cells (PBMC) and in unstimulated PBMC cultures which increased further following stimulation with phorbol myristate acetate (PMA)/Ca in vitro. In contrast, expression of IFN-7 mRNA in controls was only detected in stimulated cultures, as has been demonstrated previously for normal adults. These findings demonstrate ihat circulating T cells from atopic children have been activated in vivo, and suggest that T cell activation is a significant component of Ihe inflammatory process in dVopic dermatitis. Although expression of IFN-7 mRNA was increased in the atopic children, secretion was confirmed to be significantly lower than in controls, indicating that the defect(.'i) underlying reduced IFN-7 secretion in these individuals lie post-transcriptionally.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 90%

Reduced interferon-gamma (IFN-γ) secretion with increased IFN-γ mRNA expression in atopic dermatitis: evidence for a post-transcriptional defect

Tang

Varigos

Kemp

1994

Clinical and Experimental Immunology

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“…It has previously been reported that nonspecific polyclonal activation with a surrogate antigen induces a Th lymphocyte cytokine response similar to that produced by allergen-specific stimulation. 28,29 Our study demonstrates that the use of a surrogate antigenic stimulus (third-party alloantigen or PHA), although not as specific as the use of donor antigen, can be used for an initial testing stimulus for recipient PBMC cytokine expression by recipient PBMCs in the setting of OLT. If this initial assessment suggests an immunologic perturbation, donor alloantigens could then be used in subsequent assays of PBMC cytokine expression.…”

Section: Discussionmentioning

confidence: 93%

Assessment of Immunologic Status of Liver Transplant Recipients by Peripheral Blood Mononuclear Cells in Response to Stimulation by Donor Alloantigen

Chen¹,

McKenna²,

Yoshida³

et al. 1999

Annals of Surgery

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ObjectiveTo determine whether there is a role for assessing peripheral blood mononuclear cell (PBMC) cytokine patterns as a means of measuring the immunologic and clinical status of liver transplant recipients. Summary Background DataThe role of assessing cytokine patterns in the prediction of clinical graft rejection or acceptance remains unclear. The purpose of this study was to examine the cytokine profiles of PBMC stimulated in vitro with donor alloantigen and to correlate prospectively the data with clinical assessment of graft status in orthotopic liver transplant (OLT) recipients. MethodsPBMCs from OLT recipients were examined for proliferation and cytokine mRNA expression after stimulation by donor alloantigen, third-party alloantigen, or phytohemagglutinin (PHA). mRNA extracted from PBMC was amplified by reverse transcriptase-polymerase chain reaction with oligospecific primer pairs for interleukin (IL)-2, IL-4, IL-6, IL-10, interferon (IFN) ␥, tumor necrosis factor (TNF) ␣ and transforming growth factor (TGF) ␤. Results were prospectively correlated with each patient's allograft status. ResultsIncreased IL-4 and TGF-␤ and decreased IL-2, IFN␥, and TNF-␣ mRNA expression by PBMCs in response to donor alloantigen stimulation predicted immunologic graft stability over a minimum 60-day interval compared with mRNA expression of PBMCs from patients with established rejection or those who experienced a rejection episode within a 30-day period (p Ͻ 0.05). Stimulation of recipient PBMCs with thirdparty alloantigens or PHA yielded similar but less specific results. PBMC proliferation to varying antigenic stimulation did not correlate with clinical graft status, nor did cytokine production by unstimulated PBMC. ConclusionsProspective assessment of cytokine expression by PBMC from OLT recipients in response to stimulation by donor alloantigen is helpful for predicting the clinical status of the allograft and may be useful in the development of more precise immunologic monitoring protocols.Current immunosuppressive agents have allowed for marked improvements in the results of short-and longterm outcomes in clinical transplantation, but their use is associated with risks of opportunistic infections, malignancy, and other metabolic complications. Immunosuppression currently relies on the empirical use of these nonspecific immunosuppressive agents. Further, precise immunologic monitoring to determine the appropriate dose of drugs is not yet available. Studies examining methods to optimize the administration of immunosuppression are of significant interest, as are studies examining potential mechanisms involved in the induction of allograft hyporesponsiveness.

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“…[13] Interleukin (IL)-4 production was usually[14] but not always[15] reported as substantially higher. In some studies, neither IL-4 nor IFN-γ production was different,[716] whereas in others, synthesis of both cytokines was affected, with IFN-γ inhibited and IL-4 elevated[717] relative to the responses elicited in normal controls. Some of these discrepancies may reflect different etiologies for related but distinct hypersensitivity states, i.e., atopic dermatitis versus allergic rhinitis.…”

Section: Introductionmentioning

confidence: 99%

“…Some of these discrepancies may reflect different etiologies for related but distinct hypersensitivity states, i.e., atopic dermatitis versus allergic rhinitis. [71011121314151617] However, the variation frequently observed by different groups studying the same disease may also stem from the fact that many studies have been carried out with very small numbers of subjects. [7] Despite the availability of modern approaches, these discrepancies in cytokines productions under allergic disorders have not been fully clarified.…”

Section: Introductionmentioning

confidence: 99%

Interleukin-4 and interferon-γ are possible allergic markers in pediatric patients with β-lactam hypersensitivity

Mahmoud

Alzolibani

Rasheed

et al. 2016

Int J App Basic Med Res

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Background:β-lactam agents are known to elicit T-cell-mediated immune responses that play a central role in the onset of allergic reactions, but the involvement of specific type of cytokines in drug allergy remains largely unexplored in humans.Objectives:This study was undertaken to investigate the role of cytokines involvement in pediatric patients with β-lactam hypersensitivity and to determine whether involvement of cytokines in drug-mediated reactions are important for the perspective of allergic patient's management.Methods:β-lactam-induced hypersensitivity reactions in eighty pediatric patients were determined by clinical manifestations and skin prick or intradermal testing. Production of T-helper (Th) type-1 cytokine interferon (INF)-γ, Th-2 cytokine interleukin (IL)-4, regulatory T-cell cytokine IL-10, and other cytokines IL-6 and IL-12 were determined by sandwich ELISAs.Results:Diagnosis of β-lactam allergy was confirmed in 53 pediatric patients. IL-4 secretion in patients' sera was significantly higher as compared with healthy controls (P < 0.05). However, INF-γ level in patients' sera was significantly lower as compared with controls (P < 0.05). No significant alterations were found in the protein secretion of IL-10, IL-12, and IL-6 in allergic patients as compared with controls (P > 0.05).Conclusion:We conclude that IL-4 is specific marker for the diagnosis of β-lactam-induced hypersensitivity. Moreover, IL-4 in combination with INF-γ is more sensitive for the diagnosis of these reactions. This study also concludes that both IL-4 and INF-γ may play an active role in the onset of allergic reactions against β-lactam antibiotics.

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Detection of Cytokine mRNA in Polyclonally‐, Antigen‐ or Allergen‐Stimulated Mononuclear Cells

Cited by 7 publications

References 46 publications

Reduced interferon-gamma (IFN-γ) secretion with increased IFN-γ mRNA expression in atopic dermatitis: evidence for a post-transcriptional defect

Reduced interferon-gamma (IFN-γ) secretion with increased IFN-γ mRNA expression in atopic dermatitis: evidence for a post-transcriptional defect

Assessment of Immunologic Status of Liver Transplant Recipients by Peripheral Blood Mononuclear Cells in Response to Stimulation by Donor Alloantigen

Interleukin-4 and interferon-γ are possible allergic markers in pediatric patients with β-lactam hypersensitivity

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