Detection of cytokeratin 20 mRNA in the peripheral blood of patients with colorectal cancer by immunomagnetic bead enrichment and real‐time reverse transcriptase–polymeras chain reaction

Guo, Junming; Xiao, Bingxiu; Jin, Zhijin; Qin, Lijun; Chen, Jian; Chen, Hui; Zhang, Xinjun; Zhong, Lei

doi:10.1111/j.1440-1746.2005.03894.x

Cited by 23 publications

(24 citation statements)

References 30 publications

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“…This step will allow the direct visualisation and quantification of isolated tumour cells. Alternatively, PCR-based analyses may allow the evaluation of expression of cancerspecifi c genes from blood samples [12,13]. Molecular-based approaches have been very popular for the detection of CTCs [14,15].…”

Section: Methods For the Detection And Isolation Of Ctcsmentioning

confidence: 99%

Clinical relevance associated to the analysis of circulating tumour cells in patients with solid tumours

et al. 2009

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The distant growth of tumour cells escaping from primary tumours, a process termed metastasis, represents the leading cause of death among patients affected by malignant neoplasias from breast and colon. During the metastasis process, cancer cells liberated from primary tumour tissue, also termed circulating tumour cells (CTCs), travel through the circulatory and/or lymphatic systems to reach distant organs. The early detection and the genotypic and phenotypic characterisation of such CTCs could represent a powerful diagnostic tool of the disease, and could also be considered an important predictive and prognostic marker of disease progression and treatment response. In this article we discuss the potential relevance in the clinic of monitoring CTCs from patients suffering from solid epithelial tumours, with emphasis on the impact of such analyses as a predictive marker for treatment response.

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Section: Methods For the Detection And Isolation Of Ctcsmentioning

confidence: 99%

Clinical relevance associated to the analysis of circulating tumour cells in patients with solid tumours

et al. 2009

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“…In gastric cancer, the detection rate of CTC in blood ranged from 0% to 53.3%, and the correlation between CTC and recurrence or prognosis was found in 4 of 7 studies [15][16][17][18][19][20][21]. In colorectal cancer, the detection rate of CTC in blood ranged from 15.0% to 72.5%, and a relationship between CTC and recurrence or prognosis was found in 8 of 13 studies [22][23][24][25][26][27][28][29][30][31][32][33][34]. The reason for the different results was perhaps the various primers used for the RT-PCR method.…”

Section: Conclusion and Perspectivementioning

confidence: 97%

Circulating tumor cells in blood of esophageal squamous cell carcinoma

et al. 2007

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“…This study has been corroborated by a number of other studies. 82,100 A recent study performed by Guo and coworkers 101 detected CK20 mRNA intracellularly in 72.5 to 82.5 percent in CTC of 40 patients with CRC by using a combination of positive and negative immunomagnetic isolation and real-time PCR. They demonstrated that CK20 mRNA presence in CTC correlates significantly with the tumor size and lymphatic and hepatic invasion but not the grade of differentiation.…”

Section: Human Telomerase Reverse Transcriptase Mrnamentioning

confidence: 98%

Epithelial Molecular Markers in the Peripheral Blood of Patients with Colorectal Cancer

Khair¹,

Monson²,

Greenman³

2007

Diseases of the Colon &Amp; Rectum

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Despite the modest improvements in patient survival from colorectal cancer in the last few decades, the overall five-year survival rate remains at 40 to 45 percent. Surgical resection is the mainstay of treatment for colorectal cancer; however, nearly one-half of all patients who undergo a potentially curative resection will relapse because of undetected micrometastasis. The fact that the overall survival rate remains poor strongly suggests that the dissemination of these cells occurs early in the disease process and emphasizes the need for finding feasible diagnostic methods with sufficient sensitivity and specificity. The most commonly used technique for the detection of nucleic acid material of disseminated tumor cells is the polymerase chain reaction. We critically review the literature on DNA and messenger ribonucleic acid molecular markers that have been used for the detection of circulating tumor cells in the peripheral blood of patients with colorectal cancer and other solid tumors as appropriate for comparison. The cytokeratins, particularly cytokeratin 19 and cytokeratin 20, are the most investigated prognostic markers, but even for these questions remain about their clinical value, and hence most recent studies are utilizing a combination of factors. There is an urgent need for standardized isolation and analysis techniques to be adopted thus allowing large-scale, appropriately controlled, multicenter trials to be undertaken on the most promising candidate markers.

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Detection of cytokeratin 20 mRNA in the peripheral blood of patients with colorectal cancer by immunomagnetic bead enrichment and real‐time reverse transcriptase–polymeras chain reaction

Abstract: The combined use of negative and positive immunomagnetic beads followed by amplification of CK20 mRNA by means of RT-PCR is a non-invasive, sensitive, and specific assay for the detection of circulating colonic cancer cells.

Cited by 23 publications

References 30 publications

Clinical relevance associated to the analysis of circulating tumour cells in patients with solid tumours

Clinical relevance associated to the analysis of circulating tumour cells in patients with solid tumours

Circulating tumor cells in blood of esophageal squamous cell carcinoma

Epithelial Molecular Markers in the Peripheral Blood of Patients with Colorectal Cancer

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