Detection of Cytochrome P450-2A6, -3A5 and -4B1 with Real-Time Polymerase Chain Reaction in Prostate Tissue

Zencir, Sevil; Alptekin, Davut; Çeliktaş, Medih; Canturk, Pakize; Çolak, Deniz; Caner, Vildan; Lüleyap, Ümit; Topçu, Zeki

doi:10.1515/znc-2008-9-1026

Cited by 4 publications

(3 citation statements)

References 15 publications

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“…It was found that CYP4B1 mRNA was detected in 11 of 21 prostate cancer samples (60%) and 50% of the control samples. 15…”

Section: Prostate Cancermentioning

confidence: 99%

“…9,10 The contribution of CYP4B1 in cancer is of particular interest as CYP4B1 gene expression was found to be altered and presumably associated with certain cancers. 7,[11][12][13][14][15][16][17] On the contrary, several studies reported that human CYP4B1 was inactive due to a loss of function substitution of proline with serine at position 427 (p.S427P). 10 Therefore, it remains dubious that the low levels of CYP4B1 have functional consequences in normal or cancerous cells.…”

Section: Introductionmentioning

confidence: 99%

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Cytochrome P450 4B1 (CYP4B1) as a target in cancer treatment

et al. 2020

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Cytochrome P450 4B1 (CYP4B1) plays crucial roles in biotransforming of xenobiotics. Its predominant extrahepatic expression has been associated with certain tissue-specific toxicities. However, the expressions of CYP4B1 in various cancers and hence their potential roles in cancer development were inclusive. In this work, existing knowledge on expression and regulation of CYP4B1 gene and protein, catalysis of CYP4B1, association of CYP4B1 with cancers, contradicting findings about human CYP4B1 activities as well as the employing CYP4B1 in suicide gene approach for cancer treatment were reviewed. To date, it appears that there is a wide spectrum of tissue distribution of CYP4B1 with lungs as the predominant sites. Several nuclear receptors are possibly responsible for regulating its gene expression. The involvement of CYP4B1 in cancer was considered via activation of procarcinogens and neovascularization. However, human CYP4B1 was found to be inactive due to a substitution of proline with serine at position 427. Suicide gene approach combining reengineered CYP4B1 and prodrug 4-ipomeanol (4-IPO) has shown a promising potential for targeted cancer therapy. Further studies should focus on the verification of human CYP4B1 catalytic activities. More compounds with similar structure as 4-IPO should be tested to identify more alternative agents for the suicide gene approach in cancer treatment.

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“…It was found that CYP4B1 mRNA was detected in 11 of 21 prostate cancer samples (60%) and 50% of the control samples. 15…”

Section: Prostate Cancermentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cytochrome P450 4B1 (CYP4B1) as a target in cancer treatment

et al. 2020

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show abstract

“…Moreover, gene expression analyses demonstrated the suppression of CYP4B1 in adrenocortical tumorigenesis [110]. CYP4B1 mRNA was also detected in human prostate samples [111,112]; 60% of the prostate cancer samples were positive for CYP4B1 mRNA while in non-cancerous prostate samples, only 50% were positive for CYP4B1 mRNA [112]. Similarly, CYP4B1 could also be detected in breast and tumor breast tissues, with 76.9% or 84.6%, respectively [113], thus suggesting an increased risk of developing prostate or breast cancer with increased CYP4B1 expression.…”

Section: Tissue Distribution Of Cyp4b1 Single Nucleotide Polymorphism...mentioning

confidence: 94%

Spotlight on CYP4B1

Röder

Hüsken

Hutter

et al. 2023

IJMS

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The mammalian cytochrome P450 monooxygenase CYP4B1 can bioactivate a wide range of xenobiotics, such as its defining/hallmark substrate 4-ipomeanol leading to tissue-specific toxicities. Similar to other members of the CYP4 family, CYP4B1 has the ability to hydroxylate fatty acids and fatty alcohols. Structural insights into the enigmatic role of CYP4B1 with functions in both, xenobiotic and endobiotic metabolism, as well as its unusual heme-binding characteristics are now possible by the recently solved crystal structures of native rabbit CYP4B1 and the p.E310A variant. Importantly, CYP4B1 does not play a major role in hepatic P450-catalyzed phase I drug metabolism due to its predominant extra-hepatic expression, mainly in the lung. In addition, no catalytic activity of human CYP4B1 has been observed owing to a unique substitution of an evolutionary strongly conserved proline 427 to serine. Nevertheless, association of CYP4B1 expression patterns with various cancers and potential roles in cancer development have been reported for the human enzyme. This review will summarize the current status of CYP4B1 research with a spotlight on its roles in the metabolism of endogenous and exogenous compounds, structural properties, and cancer association, as well as its potential application in suicide gene approaches for targeted cancer therapy.

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Recurrent CYP2A6 gene mutation in biphasic hyalinizing psammomatous renal cell carcinoma: Additional support of three cases

Ding

Zhang

et al. 2023

Pathology - Research and Practice

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Detection of Cytochrome P450-2A6, -3A5 and -4B1 with Real-Time Polymerase Chain Reaction in Prostate Tissue

Cited by 4 publications

References 15 publications

Cytochrome P450 4B1 (CYP4B1) as a target in cancer treatment

Cytochrome P450 4B1 (CYP4B1) as a target in cancer treatment

Spotlight on CYP4B1

Recurrent CYP2A6 gene mutation in biphasic hyalinizing psammomatous renal cell carcinoma: Additional support of three cases

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