Detection of Cytochemical and Morphological Anomalies in ‘Preleukemia’

Abstract

The present investigations confirm and extend previous reports on the occurrence of cytochemical anomalies among the hemopoietic cells in preleukemia. The cytochemical patterns of the hemopoietic cells obtained from 19 patients suffering from a preleukemic disorder have been studied. The diagnostic value of the cytochemical examination using a panel of 10 different tests is emphasized. In a prospective study in 17 out of 25 patients, preleukemia had been correctly diagnosed. In 4 patients leukemia developed within 2–4 months from the diagnosis (‘imminent leukemia’), in 13 patients leukemia or smouldering leukemia developed between 4 and 25 months after the diagnosis (‘true preleukemia’). The cytochemical profiles of the hemopoietic cells of the latter have been compared with those of 14 patients suffering from aplastic anemia. In preleukemia the blasts did not exceed 3% of the nucleated bone marrow cells. The clinical value of the diagnosis ‘preleukemia’ is discussed as well as the prognosis and the possible therapeutic approaches.

Studies in a Case of Bernard-Soulier Syndrome

“…preleukaemic myelodysplasia, intoxication by cytotoxic agents and chronic alcoholic myelodys plasia [34], All of these could be excluded in this case. We did not find such anoma lies in congenital benign neutropenia [33].…”

“…The re- peated cytochemical examinations of the haemopoietic cells of the sister showed distinct alterations of the distribution of peroxidase and neutral protease (naphthol-AS-D-chloroacetate esterase) activi ties, thus indicating an abnormal assembly of the primary granules. Similar maturational defects can be observed in a variety of primary and secondary myelodysplastic syndromes [33,34], i.e. preleukaemic myelodysplasia, intoxication by cytotoxic agents and chronic alcoholic myelodys plasia [34], All of these could be excluded in this case.…”

Esterase cytochemistry in primary myelodysplastic syndromes and megaloblastic anaemias: demonstration of abnormal staining patterns associated with dysmyelopoiesis

“…There are few previous studies of esterase cytochemistry in MDS. Rydell(l979) observed two cases of haemopoietic dysplasia in which apparent granulocytes unexpectedly showed signifcant NSE positivity whilst Schmalzl et al (1978) in a larger study reported no…”

Αιματολογικη Και Καρυοτυπικη Μελετη Εικοσι Περιπτωσεων Προλευχαιμιας

“…Τοϋτο ύποδηλοΐ τήν παρουσίαν δύο κλώνων κυττά ρων, ενός φυσιολογικού καί ενός λευχαιμικού (Lehrer et al 1972-Breton et al 1975. Ή αλκαλική φωσφατάση των λευκών παρατηρείται ήλαττωμένη εις τό 20% περίπου των προλευχαιμικών ασθενών, ή μέτρησίς της όμως στερείται ειδικής διαγνωστικής (Schmalzi et al 1978).…”