Detection of Cysticercus cellulosae Antigens in Cerebrospinal Fluid by Dot Enzyme-Linked Immunosorbent Assay (Dot-ELISA) and Standard ELISA

E, Tellez-Giron; Ramos, Martha C.; Dufour, L; Álvarez, Pilar; Montante, M

doi:10.4269/ajtmh.1987.37.169

Cited by 30 publications

(12 citation statements)

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“…The sensitivity of the present ELISA when used with the 1:800 threshold (89%) is similar to, or better than, those of the ELISA used to check children in the U.S.A. (Kossoff, 2003), children (Idris et al, 1984) and adults (Corona et al, 1986;Larralde et al, 1986;Proano-Narvaez et al, 2002) in Mexico, and adults in Chandigarh, India (Kaur et al, 1996). The sensitivity of the present dot-blot assay (also 89%) was similar to that of the dot-blot assay used, on sera, by Plancarte et al (1994) in Mexicoeven though the latter assay was based on a purified GP 24 antigenic fraction, not a crude antigenic extract -and much better than the value (59%) reported for the dotblot assay used, by Tellez-Giron et al (1987), on CSF samples in Mexico.…”

Section: Discussionsupporting

confidence: 71%

“…Dot-blots have been used for the serodiagnosis of several parasitic infections and can give sensitivities as high as those observed with ELISA, while being rapid and easy to read (Pappas et al, 1983;Brooks et al, 1985;Tellez-Giron et al, 1987;Mahajan et al, 1989). When Tellez-Giron et al (1987) compared the performances of dot-ELISA and standard ELISA, in the detection of Cysticercus cellulosae antigens in samples of cerebrospinal fluid (CSF) from patients in Mexico, they found both assays to be reasonably sensitive, 100% specific and cost-effective for the routine diagnosis of NCC.…”

mentioning

confidence: 99%

“…When Tellez-Giron et al (1987) compared the performances of dot-ELISA and standard ELISA, in the detection of Cysticercus cellulosae antigens in samples of cerebrospinal fluid (CSF) from patients in Mexico, they found both assays to be reasonably sensitive, 100% specific and cost-effective for the routine diagnosis of NCC.…”

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confidence: 99%

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Evaluation of ELISA and dot blots for the serodiagnosis of neurocysticercosis, in children found to have single or multiple enhancing lesions in computerized tomographic scans of the brain

Mandal

Singhi

Khandelwal

et al. 2006

Annals of Tropical Medicine & Parasitology

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Although human neurocysticercosis (NCC) is being increasingly recognized in children, diagnosis of the disease can be difficult, and the 'gold standard' criteria that indicate an unambiguous case have still to be established. In the present study, the performances of an ELISA and dot-blot assay, for the detection of antibodies against antigens from larval Taenia solium, were investigated and compared, using sera, from children aged 5-12 years, that were diluted to at least 1:400. Eighty of the subjects (20 aged 5-<8 years and 60 older children) each had the signs and symptoms of NCC, including one brain lesion (N=69) or multiple brain lesions (N=11) that were visible by computed tomography. Another 100 sera, from children who had tubercular meningitis (N=20) or a parasitic disease other than taeniasis/cysticercosis (N=20) or, apart from a minor respiratory-tract infection, appeared healthy (N=60), were also investigated. Most (86%) of the cases of NCC had presented with focal seizures. Analysis of antibody response indicated that the optimum threshold titres for seropositivity were 1:800 for the ELISA and 1:6400 for the dot-blot assays. When used with these thresholds, the ELISA gave a sensitivity, specificity, positive and negative predictive values and diagnostic efficacy of 89%, 81%, 79%, 90%, 85%, respectively. The corresponding values for the dot-blot assay were similar, at 89%, 73%, 72.5%, 89%, 82%, respectively. Both assays were more sensitive, in the detection of the specific antibody response, when used among the paediatric cases of NCC who had multiple brain lesions (100%) than when used among the single-lesion cases (87%). As the ELISA gave higher specificity and diagnostic efficacy than the dot-blot assay, it should be considered the better method for the serological confirmation of NCC in children.

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Section: Discussionsupporting

confidence: 71%

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confidence: 99%

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Evaluation of ELISA and dot blots for the serodiagnosis of neurocysticercosis, in children found to have single or multiple enhancing lesions in computerized tomographic scans of the brain

Mandal

Singhi

Khandelwal

et al. 2006

Annals of Tropical Medicine & Parasitology

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“…Their results were promising, particularly in terms of specificity (likely resulting from the use of CSF instead of serum, as detailed above). 129,130 As expected, circulating antigen cannot be demonstrated in the CSF of all NCC patients. Also, only a fraction of all antigens present in the cyst fluid can be detected in the patient's CSF.…”

Section: Antigen Detection Tests For Cysticercosissupporting

confidence: 62%

Immunological and molecular diagnosis of cysticercosis

Rodríguez¹,

Wilkins

Dorny

2012

Pathogens and Global Health

126

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Cysticercosis, the infection with the larval stage of Taenia solium, is a cause of neurological symptoms including seizures, affecting the quality of life of patients and their families. Diagnosis focuses on brain imaging and serological tests are mostly used as confirmatory tools. Most cases, however, occur in poor endemic areas, where both kinds of diagnostic tools are poorly available. Development of point of care diagnostic tests is one of the most important priorities for cysticercosis researches today. The ideal point of care test would require detection of viable cysticercosis and hopefully identify cases with severe or progressive forms of neurocysticercosis, leading to referral of the patient for specialized medical attention. This manuscript describes the evolution of the serological diagnosis of cysticercosis over time, and the characteristics of the most common currently available tools, their advantages and disadvantages, and their potential use in future diagnostic tests.

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“…Among the high molecular weight peptides, 131, 128 and 70 kDa peptides were found to be specific in their detection in CSF as recognized by the anti‐somatic antibody. High molecular peptides (66, 190, 230 and 150 kDa) in CSF were also reported to be diagnostic by some other studies . Estrada et al .…”

Section: Discussionmentioning

confidence: 72%

Comparative profile of circulating antigenic peptides in CSF, serum & urine from patients with neurocysticercosis diagnosed by immunoblotting

Sahu

Kumar³

et al. 2014

Parasite Immunology

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Traditionally serum and/or CSF specimens have been used for detection of either specific antibodies or antigens as a supportive diagnosis of NCC. However, in recent days, much interest has been shown employing noninvasive specimens such as urine. In our study, we identified and compared a profile of circulating antigenic peptides of parasite origin in three different body fluids (CSF, serum and urine) obtained from confirmed NCC cases and control subjects. The circulating antigenic peptides were resolved by SDS-PAGE and subjected to immunoblotting. For confirmation of their origin as parasite somatic or excretory secretory (ES) material, immunoreactivity was tested employing affinity purified polyclonal Taenia solium metacestode anti-somatic or ES antibodies, respectively. Only lower molecular weight antigenic peptides were found circulating in urine in contrast to serum and CSF specimens. Few somatic peptides were identified to be 100% specific for NCC (19·5 kDa in all three specimens; 131, 70 kDa in CSF and serum only; 128 kDa in CSF only). Similarly, the specific ES peptides detected were 32 kDa (in all three specimens), 16·5 kDa (in serum and CSF only), and 15 kDa (urine only). A test format detecting either one or more of these specific peptides would enhance the sensitivity in diagnosis of NCC.

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Detection of Cysticercus cellulosae Antigens in Cerebrospinal Fluid by Dot Enzyme-Linked Immunosorbent Assay (Dot-ELISA) and Standard ELISA

Cited by 30 publications

References 0 publications

Evaluation of ELISA and dot blots for the serodiagnosis of neurocysticercosis, in children found to have single or multiple enhancing lesions in computerized tomographic scans of the brain

Evaluation of ELISA and dot blots for the serodiagnosis of neurocysticercosis, in children found to have single or multiple enhancing lesions in computerized tomographic scans of the brain

Immunological and molecular diagnosis of cysticercosis

Comparative profile of circulating antigenic peptides in CSF, serum & urine from patients with neurocysticercosis diagnosed by immunoblotting

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