Detection of Culex flavivirus and Aedes flavivirus nucleotide sequences in mosquitoes from parks in the city of São Paulo, Brazil

Fernandes, Lícia Natal; Paula, Márcia Bicudo de; Araújo, Alessandra Bergamo; Gonçalves, Elisabeth Fernandes Bertoletti; Romano, Camila Malta; Natal, Délsio; Malafronte, Rosely dos Santos; Marrelli, Mauro Toledo; Levi, José Eduardo

doi:10.1016/j.actatropica.2016.01.026

Cited by 15 publications

(22 citation statements)

References 83 publications

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“…Therefore, despite the negative results, our study could not rule out the presence of Flavivirus species circulating among birds in these areas. These fi ndings supported those reported in another study, in which the presence of Flavivirus of medical importance was not detected in mosquitoes captured in several municipal parks in São Paulo (15) . Due to their potential for harboring Flavivirus species, it would be necessary to capture more birds for better Flavivirus surveillance.…”

supporting

confidence: 91%

A survey of flaviviruses in birds in green areas of São Paulo, Brazil

Orico

Fernandes

Melo

et al. 2016

Rev. Soc. Bras. Med. Trop.

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supporting

confidence: 91%

A survey of flaviviruses in birds in green areas of São Paulo, Brazil

Orico

Fernandes

Melo

et al. 2016

Rev. Soc. Bras. Med. Trop.

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“…Mosquito-borne, tick-borne and unknown vector flaviviruses are placed in a phylogenetic sister group to the classical ISVs within the Flavivirus genus, which includes CxFV (Fernandes et al, 2016). Studies have shown that prior infection by CxFV can suppress the replication of dual-flavivirus infections in mosquito cell lines (Kenney et al, 2014;Bolling et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…Culex flavivirus (CxFV) has been isolated from Culex pipiens Linnaeus 1758 complex in several countries (Hoshino et al, 2007;Moralles-Betoulle et al, 2008;Cook et al, 2009). In Brazil, this virus was previously identified in mosquitoes from the cities of São José do Rio Preto and São Paulo, both in the state of São Paulo (Machado et al, 2012;Fernandes et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Natural infection by Culex flavivirus in Culex quinquefasciatus mosquitoes captured in Cuiabá, Mato Grosso Mid‐Western Brazil

Moraes

Cardoso

Pacheco

et al. 2019

Medical Vet Entomology

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New species of insect‐specific viruses (ISV) have been reported worldwide. In the present study, the complete genome of Culex flavivirus (CxFV) and partial sequences of other ISVs in Culex quinquefasciatus Say 1823 females (n = 3425) sampled in 200 urban areas census tracts of Cuiaba, state of Mato Grosso, were identified via reverse transcriptase‐polymerase chain reaction for a NS5 region of flaviviruses, nucleotide and high‐throughput sequencing, and viral isolation in C6/36 cells. CxFV was detected in 16 of 403 mosquito pools; sequences found in the study presented a high similarity with isolates from São Paulo, Brazil and other countries in Latin American that belong to genotype II, supporting the geographical influence on CxFV evolution. The monthly maximum likelihood estimation for CxFV ranged from 1.81 to 9.94 per 1000 mosquitoes. In addition to the CxFV complete genome, one pool contained an ORF1 sequence (756 bp) that belongs to a novel Negevirus from the Sandewavirus supergroup most similar to the Santana virus (77.1%) and another pool presented an RNA‐dependent RNA polymerase sequence (1081 bp) of a novel Rhabdovirus most similar to Wuhan mosquito virus 9 (44%). After three passages in C6/36 cells, only CxFV was isolated from these co‐infected pools. The importance of ISVs relies on their possible ability to interfere with arbovirus replication in competent vectors.

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“…This species specific infection led to the putative agent being designated AeFV (Hoshino et al, 2009). Since this original identification, AeFV has been found in both Europe (Roiz et al, 2012) and the Americas (Fernandes et al, 2016;Haddow et al, 2013), and has demonstrated some evidence of vertical/TOT due to its presence in male mosquitoes.…”

Section: Aedes Flavivirusmentioning

confidence: 97%