Detection of CTX-M-15 harboring Escherichia coli isolated from wild birds in Tunisia

Yahia, Houssem Ben; Sallem, Rym Ben; Tayh, Ghassan; Klibi, Naouel; Amor, Insaf Ben; Gharsa, Haythem; Boudabbous, A.; Slama, Karim Ben

doi:10.1186/s12866-018-1163-2

Cited by 26 publications

(19 citation statements)

References 63 publications

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“…Isolation of CR-carrying bacteria from wild birds has been globally reported in E . coli [15–20] and less frequently in Klebsiella pneumoniae [21]. All these results confirm the dissemination success of ESBL bla SHV-12 and bla CTX-M variants in wild birds worldwide.…”

Section: Introductionsupporting

confidence: 58%

High prevalence and diversity of extended-spectrum β-lactamase and emergence of OXA-48 producing Enterobacterales in wildlife in Catalonia

et al. 2019

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Most of the studies focused on antimicrobial resistance (AMR) performed in wildlife describe Escherichia coli as the principal indicator of the selective pressure. In the present study, several species of Enterobacterales with a large panel of cephalosporin resistant (CR) genes have been isolated from wildlife in Catalonia. A total of 307 wild animals were examined to determine the prevalence of CR enterobacteria, AMR phenotypes and the presence of common carbapenem and CR genes. The overall prevalence of CR-phenotype was 13% (40/ 307): 17.3% in wild mammals (18/104) and 11.5% in wild birds (22/191) (p<0.01). Hedgehogs showed the highest prevalence (13.5% of 104) of the mammal specimens, and raptors the highest in bird specimen (7.3% of 191). Although CR E. coli was the most frequently isolated (45%), other CR-Enterobacterales like Klebsiella pneumoniae (20%), Citrobacter freundii (15%), Enterobacter cloacae (5%), Proteus mirabilis (5%), Providencia spp (5%) and Serratia marcescens (2.5%) were also isolated. A high diversity of CR genes was identified among the isolates, with 50% yielding blaCMY-2, 23% blaSHV-12, 20% blaCMY-1 and 18% blaCTX-M-15. Additionally, resistance to carbapenems associated to OXA-48 gene was found. Most of the CR isolates, principally K. pneumoniae and C. freundii, were multiresistant with co-resistance to fluoroquinolones, tetracycline, sulphonamides and aminoglycosides. This study reports high prevalence of Enterobacterales harbouring a variety of CR genes and OXA-48 mediated-carbapenem resistance, all of them frequently associated to nosocomial human infections, for the first time in wild mammals and wild birds. Implementation of control measures to reduce the impact of anthropogenic pressure in the environment is urgently needed.

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Section: Introductionsupporting

confidence: 58%

High prevalence and diversity of extended-spectrum β-lactamase and emergence of OXA-48 producing Enterobacterales in wildlife in Catalonia

et al. 2019

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“…However, most of the environmental-wildlife interface studies have been focused on wild birds, as principal AMR disseminators by their migratory routes, with a limited variety of AMR bacteria species described. Isolation of CR-carrying bacteria from wild birds has been globally reported in Escherichia coli [12-17] and less frequently in Klebsiella pneumoniae [18]. All these results confirm the dissemination success of ESBL bla SHV-12 and bla CTX-M variants in wild birds worldwide.…”

Section: Introductionsupporting

confidence: 57%

“…Studies performed in the Iberian Peninsula in wildlife, have reported bla CTX-M-1 as the main ESBL gene circulating [19,26]. Additionally, bla CTX-M-14a and bla SHV-12 have also been frequently detected in E. coli from wild animals [12,27-29] with bla CTX-M-15 described in a recent study carried out in wild birds in Tunisia [17]. Interestingly, in the present study, bla CTX-M-1 and bla CTX-M-14a were never detected, whereas bla CMY-2, bla SHV- 12 and bla CTX-M-15 were frequently isolated not only in E. coli but also in K. pneumoniae and C. freundii isolates.…”

Section: Discussionmentioning

confidence: 99%

High prevalence and diversity of Extended-Spectrum β-Lactamase and emergence of Carbapenemase producingEnterobacteriaceaespp in wildlife in Catalonia

Darwich

Vidal

Seminati

et al. 2019

Preprint

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19In wildlife, most of the studies focused on antimicrobial resistance (AMR) describe 20 Escherichia coli as the principal indicator of the selective pressure. In the present study, 21 new species of Enterobacteriaceae with a large panel of cephalosporin resistant (CR) 22 genes have been isolated from wildlife in Catalonia. A total of 307 wild animals were 23 examined to determine CR enterobacteria prevalence, AMR phenotypes and common 24 carbapenem and CR gene expression. The overall prevalence of CR-phenotype was 25 13% (40/307): 17.3% in wild mammals (18/104) and 11.5% in wild birds (22/191) 26 (p<0.01)). Hedgehogs presented the largest prevalence with 13.5% (14/104) of the 27 mammal specimens, followed by raptors with 7.3% (14/191) of the total bird specimens. 28Although CR E. coli was obtained most frequently (45%), other CR- 29Enterobacteriaceae spp like Klebsiella pneumoniae (20%), Citrobacter freundii (15%), 30 Enterobacter cloacae (5%), Proteus mirabilis (5%), Providencia spp (5%) and Serratia 31 marcescens (2.5%) were isolated. A high diversity of CR genes was identified among 32 the isolates, with 50% yielding blaCMY-2, 23% blaSHV-12, 20% blaCMY-1 and 18% 33 blaCTX-M-15. Additionally, new CR-gene variants and resistance to carbapenems 34 associated to OXA-48 were found. Most of the CR isolates, principally K. pneumoniae 35 and C. freundii, were multiresistant with co-resistance to fluoroquinolones, tetracycline, 36 sulphonamides and aminoglycosides. This study describes for the first time in wildlife a 37 high prevalence of Enterobacteriaceae spp harbouring a large variety of carbapenem 38 and CR genes frequently associated to nosocomial human infections. Implementation of 39 control measures to reduce the impact of anthropogenic pressure in the environment is 40 urgently needed. 41 In the last decades, the prevalence of opportunistic and antimicrobial resistant (AMR) 44 bacteria associated with nosocomial infections has suffered an important increase in 45 hospital settings. The overuse of antibiotics in human and veterinary medicine have led 46 to the spread of AMR pathogens, becoming a global health problem [1]. 47 Extended-spectrum β-lactamases (ESBLs) and plasmid-mediated AmpC-type β-48 lactamases (pAmpC) are the most common enzymes that confer resistance to broad-49 spectrum cephalosporins among members of the family Enterobacteriaceae. These β-50 lactamases have extensively diversified in response to the clinical use of new generation 51 drugs: cephalosporins, carbapenems and monobactams [2]. These enzymes are mostly 52 encoded by genes located in plasmids that can be horizontally transferred to different 53 bacteria genera [1]. Carbapenems are last-line beta-lactam antibiotics with the broadest 54 spectrum of activity. Unfortunately, carbapenems nowadays are commonly used in 55 hospital settings for the treatment of life-threatening infections caused by cephalosporin 56 resistant (CR) Enterobacteriaceae. However, the emergence of resistance to 57 carbapenems mediated by the produ...

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“…Several genetic mechanisms have been involved in the spread of ARGs, but conjugation is thought to have the greatest influence [3]. Furthermore, it has been reported that E. coli obtains antimicrobial resistance faster than other microorganisms [4]. It is especially relevant regarding the increase of β-lactam resistance in E. coli due…”

Section: Introductionmentioning

confidence: 99%

The Aquatic Ecosystem, a Good Environment for the Horizontal Transfer of Antimicrobial Resistance and Virulence-Associated Factors Among Extended Spectrum β-lactamases Producing E. coli

2020

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One of the main public health problems nowadays is the increase of antimicrobial resistance, both in the hospital environment and outside it (animal environment, food and aquatic ecosystems, among others). It is necessary to investigate the virulence-associated factors and the ability of horizontal gene transfer among bacteria for a better understanding of the pathogenicity and the mechanisms of dissemination of resistant bacteria. Therefore, the objective of this work was to detect several virulence factors genes (fimA, papC, papG III, cnf1, hlyA and aer) and to determine the conjugative capacity in a wide collection of extended-spectrum β-lactamases-producing E. coli isolated from different sources (human, food, farms, rivers, and wastewater treatment plants). Regarding virulence genes, fimA, papC, and aer were distributed throughout all the studied environments, papG III was mostly related to clinical strains and wastewater is a route of dissemination for cnf1 and hlyA. Strains isolated from aquatic environments showed an average conjugation frequencies of 1.15 × 10 −1 ± 5 × 10 −1 , being significantly higher than those observed in strains isolated from farms and food (p < 0.05), with frequencies of 1.53 × 10 −4 ± 2.85 × 10 −4 and 9.61 × 10 −4 ± 1.96 × 10 −3 , respectively. The reported data suggest the importance that the aquatic environment (especially WWTPs) acquires for the exchange of genes and the dispersion of resistance. Therefore, specific surveillance programs of AMR indicators in wastewaters from animal or human origin are needed, in order to apply sanitation measures to reduce the burden of resistant bacteria arriving to risky environments as WWTPs.

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Detection of CTX-M-15 harboring Escherichia coli isolated from wild birds in Tunisia

Cited by 26 publications

References 63 publications

High prevalence and diversity of extended-spectrum β-lactamase and emergence of OXA-48 producing Enterobacterales in wildlife in Catalonia

High prevalence and diversity of extended-spectrum β-lactamase and emergence of OXA-48 producing Enterobacterales in wildlife in Catalonia

High prevalence and diversity of Extended-Spectrum β-Lactamase and emergence of Carbapenemase producingEnterobacteriaceaespp in wildlife in Catalonia

The Aquatic Ecosystem, a Good Environment for the Horizontal Transfer of Antimicrobial Resistance and Virulence-Associated Factors Among Extended Spectrum β-lactamases Producing E. coli

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