Detection of ctx gene positive non-O1/non-O139 V. cholerae in shrimp aquaculture environments

Madhusudana, Rao B.; Surendran, P.K.

doi:10.1007/s13197-011-0374-4

Cited by 15 publications

(15 citation statements)

References 35 publications

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“…29 In this study, all the 370 V. cholerae isolates were absent for the toxic ctxAB and tcpA genes, consistent with our prior studies, 18,30,31 and some other literature. 22,[40][41][42][43] The ace, zot genes within the CTX element of pathogenic V. cholerae were also absent from all the isolates tested in this study. A similar observation was also reported based on non-O1/ O139 V. cholerae strains of environmental origins.…”

Section: Discussionmentioning

confidence: 99%

Virulence, Resistance, and Genomic Fingerprint Traits ofVibrio choleraeIsolated from 12 Species of Aquatic Products in Shanghai, China

Pan

Liang

et al. 2020

Microbial Drug Resistance

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Vibrio cholerae is a waterborne bacterium and can cause epidemic cholera disease worldwide. Continuous monitoring of V. cholerae contamination in aquatic products is imperative for assuring food safety. In this study, we determined virulence, antimicrobial susceptibility, heavy metal tolerance, and genomic fingerprints of 370 V. cholerae isolates recovered from 12 species of commonly consumed aquatic products collected from July to September of 2018 in Shanghai, China. Among the species, Leiocassis longirostris, Ictalurus punetaus, Ophiocephalus argus Cantor, and Pelteobagrus fulvidraco were for the first time detected for V. cholerae. Toxin genes ctxAB, tcpA, ace, and zot were absent from all the V. cholerae isolates. However, high occurrence of virulence-associated genes was detected, such as hapA (82.7%), hlyA (81.4%), rtxCABD (81.4%, 24.3%, 80.3%, and 80.8%, respectively), and tlh (80.5%). Approximately 62.2% of the 370 V. cholerae isolates exhibited resistance to streptomycin, followed by ampicillin (60.3%), rifampicin (53.8%), trimethoprim (38.4%), and sulfamethoxazole-trimethoprim (37.0%). Moreover, *57.6% of the isolates showed multidrug resistant phenotypes with 57 resistance profiles, which was significantly different among the 12 species (multiple antimicrobial resistance index, p < 0.001). Meanwhile, high incidence of tolerance to heavy metals Hg 2+ (69.5%), Ni 2+ (32.4%), and Cd 2+ (30.8%) was observed among the isolates. The enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR)-based fingerprinting profiles classified the 370 V. cholerae isolates into 239 different ERIC-genotypes, which demonstrated diverse genomic variation among the isolates. Overall, the results in this study meet the increasing need of food safety risk assessment of aquatic products.

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Section: Discussionmentioning

confidence: 99%

Virulence, Resistance, and Genomic Fingerprint Traits ofVibrio choleraeIsolated from 12 Species of Aquatic Products in Shanghai, China

Pan

Liang

et al. 2020

Microbial Drug Resistance

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“…Although these virulence genes are rarely detected from the non-O1/non-O139 V. cholerae isolates, ctxA , zot , and/or ace genes were detected in two of the non-O1/non-O139 isolates (VC12 and VC13) examined in this study. The occurrence of ctxA -positive non-O1/non-O139 isolates was found in India and Brazil [ 27 , 28 ].…”

Section: Discussionmentioning

confidence: 99%

“…This isolate may acquire the O1-specific virulence genes, ctxA and/or zot , by horizontal gene transfer. The presence of ctxA and/or zot genes among non-O1/non-O139 isolates demonstrated the potential of natural genetic exchange in this organism which may have occurred in aquatic ecosystems or inside the human host [ 27 , 44 ]. Interestingly, the repeat numbers of VC0436-7 locus in one of the non-O1/non-O139 isolates (VC14) were related to those of the O1 isolates.…”

Section: Discussionmentioning

confidence: 99%

Comparison of virulence gene profiles and genomic fingerprints of Vibrio cholerae O1 and non-O1/non-O139 isolates from diarrheal patients in southern Thailand

et al. 2018

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BackgroundVibrio cholerae is associated with severe watery diarrheal disease among people in many parts of the world, including the coastal provinces of Southern Thailand. There are relatively few studies focusing on the genetic characterization among V. cholerae isolates in this region. Therefore, this study aimed at exploring the presence of virulence genes and DNA fingerprints among V. cholerae O1 and non-O1/non-O139 isolates obtained from clinical samples in four southern coastal provinces during the period of 2001–2009 (n = 21).ResultsAll V. cholerae O1 isolates possessed ctxA, tcpA, zot, ace, hlyA, and vasH genes. However, only hlyA, vcsV2, and vasH genes were detected in the majority of the non-O1/non-O139 isolates. All O1 isolates showed indistinguishable PCR fingerprints by arbitrarily primed (AP)-PCR and enterobacterial repetitive intergenic consensus (ERIC)-PCR regardless of the geographical area and period of isolation. However, the multi-locus variable-number of tandem-repeat analysis (MLVA) could differentiate these O1 isolates (n = 11) into eight profiles. Isolates exhibiting an undistinguished MLVA profile also showed identical pulsed-field gel electrophoresis (PFGE). In addition, the O1 isolates were grouped into the same cluster by all methods used in this study.ConclusionsThis study demonstrated the presence of virulence genes and genetic diversity among different serogroups of V. cholerae isolates from clinical samples in southern Thailand. V. cholerae O1 isolated over a period of multiple years were genetically related, suggesting that they had a clonal origin, whereas non-O1/non-O139 isolates could have evolved independently.

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“…Our phylogenetic and genome comparison analyses between the toxigenic and non-toxigenic V. cholerae microorganisms support both of these contentions. Further work with additional variants, such as atypical El Tor [ 56 ], NRT36S [ 49 ], and CT-producing non-O1 strains [ 57 ], will be necessary to definitively gain a complete picture of the relationships between pathogenic versus non-pathogenic V. cholerae .…”

Section: Discussionmentioning

confidence: 99%

Comparative genome analysis of non-toxigenic non-O1 versus toxigenic O1 Vibrio cholerae

Mukherjee¹,

Kakarla²,

Kumar³

et al. 2014

Genomics Discov

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Pathogenic strains of Vibrio cholerae are responsible for endemic and pandemic outbreaks of the disease cholera. The complete toxigenic mechanisms underlying virulence in Vibrio strains are poorly understood. The hypothesis of this work was that virulent versus non-virulent strains of V. cholerae harbor distinctive genomic elements that encode virulence. The purpose of this study was to elucidate genomic differences between the O1 serotypes and non-O1 V. cholerae PS15, a non-toxigenic strain, in order to identify novel genes potentially responsible for virulence. In this study, we compared the whole genome of the non-O1 PS15 strain to the whole genomes of toxigenic serotypes at the phylogenetic level, and found that the PS15 genome was distantly related to those of toxigenic V. cholerae. Thus we focused on a detailed gene comparison between PS15 and the distantly related O1 V. cholerae N16961. Based on sequence alignment we tentatively assigned chromosome numbers 1 and 2 to elements within the genome of non-O1 V. cholerae PS15. Further, we found that PS15 and O1 V. cholerae N16961 shared 98% identity and 766 genes, but of the genes present in N16961 that were missing in the non-O1 V. cholerae PS15 genome, 56 were predicted to encode not only for virulence–related genes (colonization, antimicrobial resistance, and regulation of persister cells) but also genes involved in the metabolic biosynthesis of lipids, nucleosides and sulfur compounds. Additionally, we found 113 genes unique to PS15 that were predicted to encode other properties related to virulence, disease, defense, membrane transport, and DNA metabolism. Here, we identified distinctive and novel genomic elements between O1 and non-O1 V. cholerae genomes as potential virulence factors and, thus, targets for future therapeutics. Modulation of such novel targets may eventually enhance eradication efforts of endemic and pandemic disease cholera in afflicted nations.

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Detection of ctx gene positive non-O1/non-O139 V. cholerae in shrimp aquaculture environments

Cited by 15 publications

References 35 publications

Virulence, Resistance, and Genomic Fingerprint Traits ofVibrio choleraeIsolated from 12 Species of Aquatic Products in Shanghai, China

Virulence, Resistance, and Genomic Fingerprint Traits ofVibrio choleraeIsolated from 12 Species of Aquatic Products in Shanghai, China

Comparison of virulence gene profiles and genomic fingerprints of Vibrio cholerae O1 and non-O1/non-O139 isolates from diarrheal patients in southern Thailand

Comparative genome analysis of non-toxigenic non-O1 versus toxigenic O1 Vibrio cholerae

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